1887

Abstract

Virus-specific, CD8 cytotoxic T lymphocytes (CTLs) were generated in two strains of mice (BALB/c, CBA/Ca) against bluetongue virus serotype 10 (BTV-10). Recombinant vaccinia viruses (VV) expressing the individual structural and non-structural proteins of BTV were used to infect syngeneic target cells. We found that in both BALB/c () and CBA/Ca () mice, polyclonal CTL populations recognized target cells expressing the non-structural proteins better than those expressing the structural proteins. CTLs generated against other BTV serotypes also predominantly recognized the non-structural proteins. However, the extent of cross-reactivity was dependent on the background of the animals immunized. No CTLs cross-reactive to the BTV-10 heterotype were demonstrated with the panel of molecularly cloned recombinants in the haplotype. The outer capsid proteins VP2 and VP5 which vary considerably between serotypes were not recognized by heterotypic CTLs. Using this murine model we have determined which BTV proteins are the major targets of the CTL response. The implications for the design and development of subunit vaccines are discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-5-997
1996-05-01
2022-05-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/5/JV0770050997.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-5-997&mimeType=html&fmt=ahah

References

  1. Ahmed R., Byrne J. A., Oldstone M. B. A. 1984; Virus specificity of cytotoxic T lymphocytes generated during acute lymphocytic choriomeningitis virus infection: role of the H-2 region in determining cross-reactivity for different lymphocytic choriomeningitis virus strains. Journal of Virology 51:34–41
    [Google Scholar]
  2. Aitkin M., Anderson D., Francis B., Hinde J. 1989; Statistical modelling in GLIM. Oxford: Clarendon Press;
    [Google Scholar]
  3. Bachmann M. E., Kundig T. M., Freer G., Kang C. Y., Bishop D. H. L., Hengartner H., Zinkernagel R. M. 1994; Induction of protective cytotoxic T cells with viral proteins. European Journal of Immunology 24:2128–2236
    [Google Scholar]
  4. Banks T. A., Allen E. M., Dasgupta S., Sandri-Goldin R., Rouse B. T. 1991; Herpes simplex virus type 1-specific cytotoxic T lymphocytes recognize immediate-early protein ICP27. Journal of Virology 65:3185–3191
    [Google Scholar]
  5. Del Val M., Schlicht H. J., Volkmer H., Messerie M., Reddehase M. J., Koszinowski U. H. 1991; Protection against lethal cytomegalovirus infection by a recombinant vaccine containing a single nonomeric T-cell epitope. Journal of Virology 65:3641–3646
    [Google Scholar]
  6. French T. J., Marshall J. J. A., Roy P. 1990; Assembly of double-shelled, virus-like particles of bluetongue virus by the simultaneous expression of four structural proteins. Journal of Virology 64:5695–5700
    [Google Scholar]
  7. Gard G. P., Melville L. F. 1992; Results of a decade’s monitoring for orbiviruses in sentinal cattle pastured in an area of regular arbovirus activity in Northern Australia. In Bluetongue, African Horsesickness, and Related Orbiviruses, Proceedings of the 2nd International Symposium pp 85–89 Edited by Walton T. E., Osburn B. I. Florida: CRC Press;
    [Google Scholar]
  8. Ghalib H. W., Cherrington J. M., Adkison M. A., Osburn B.I. 1985a; Humoral and cellular immune response of sheep to bluetongue virus. In Bluetongue and Related Orbiviruses pp 489–496 Edited by Barber T. L., Jochim M. M. New York: Alan R. liss;
    [Google Scholar]
  9. Ghalib H. W., Schore C. E., Osburn B. I. 1985b; Immune response of sheep to bluetongue virus: in vitro induced lymphocyte blastogenesis. Veterinary Immunology and Immunopathology 10:177–188
    [Google Scholar]
  10. Gibbs E. P. J. 1993; Control of viral diseases of sheep and goats: conventional and novel vaccines. In Veterinary Vaccines pp 182–199 Edited by Pandey R., Höglund S., Prasad G. New York: Springer Verlag;
    [Google Scholar]
  11. Hill A. B., Müllbacher A., Parrish C., Coia G., Westaway E. G., Blanden R. V. 1992; Broad cross-reactivity with marked fine specificity in the cytotoxic T cell response to flaviviruses. Journal of General Virology 13:1115–1123
    [Google Scholar]
  12. Jeggo M. H., Wardley R. C. 1982; Generation of cross-reactive cytotoxic T lymphocytes following immunization of mice with various bluetongue virus types. Immunology 45:629–635
    [Google Scholar]
  13. Jeggo M. H., Wardley R. C., Taylor W. P. 1984; Role of neutralizing antibody in passive immunity to bluetongue infection. Research in Veterinary Science 36:81–85
    [Google Scholar]
  14. Jeggo M. H., Wardley R. C., Brownlie J. 1985; Importance of ovine cytotoxic T cells in protection against bluetongue virus infection. In Bluetongue and Related Orbiviruses pp 447–487 Edited by Barber T. L., Jochim M. M. New York: Alan R. Liss;
    [Google Scholar]
  15. Lobigs M., Arthur C. E., Müllbacher A., Blanden R. V. 1994; The flavivirus nonstructural protein NS3 is a dominant source of cytotoxic T cell peptide determinants. Virology 202:195–201
    [Google Scholar]
  16. Martin S., Courtney R. J., Fowler G., Rouse B. 1988; Herpes simplex virus type 1-specific cytotoxic T lymphocytes recognize virus nonstructural proteins. Journal of Virology 62:2265–2273
    [Google Scholar]
  17. Niewiesk S., Brinckmann U., Bankamp B., Sirak S., Liebert U. G., Ter Meulen V. 1993; Susceptibility to measles virus-induced encephalitis in mice correlates with impaired antigen presentation to cytotoxic T lymphocytes. Journal of Virology 67:75–81
    [Google Scholar]
  18. Obdeyn M. 1987; Bluetongue. A review of the disease. In Scientific and Technical Monograph Series vol 16 pp 1–60 Rio de Janeiro: PAHO/WHO;
    [Google Scholar]
  19. Offit P. A., Boyle D. B., Both G. W., Hill N. L., Svoboda Y. M., Cunningham S. L., Jenkins R. J., McCrae M. A. 1991; Outer capsid glycoprotein VP7 is recognized by cross-reactive rotavirusspecific, cytotoxic T lymphocytes. Virology 184:563–568
    [Google Scholar]
  20. Offit P. A., Coupar B. E. H., Svoboda Y. M., Jenkins R. J., McCrae M. A., Abraham A., Hill N. L., Boyle D. B., Andrews M. E., Both G. W. 1994; Induction of rotavirusspecific cytotoxic T lymphocytes by vaccinia virus recombinants expressing individual rotavirus genes. Virology 198:10–16
    [Google Scholar]
  21. Parrish C. R., Coia G., Hill A., Mullbacher A., Westaway E. G., Blanden R. V. 1991; Preliminary analysis of murine cytotoxic T cell responses to the proteins of the flavivirus Kunjin using vaccinia virus expression. Journal of General Virology 72:1645–1653
    [Google Scholar]
  22. Parsonson I. M. 1992; Overview of bluetongue virus infection of sheep. In Bluetongue, African Horsesickness, and Related Orbiviruses, Proceedings of the 2nd International Symposium pp 713–724 Edited by Walton T. E., Osburn B. I. Florida: CRC Press;
    [Google Scholar]
  23. Rothman A. L., Kurane I., Lai C. J., Bray M., Falgout B., Men R., Ennis F. A. 1993; Dengue virus protein recognition by virusspecific murine CD8+ cytotoxic T lymphocytes. Journal of Virology 67:801–806
    [Google Scholar]
  24. Ritter D. G., Roy P. 1988; Genetic relationships of bluetongue virus serotypes isolated from different parts of the world. Virus Research 11:33–47
    [Google Scholar]
  25. Roy P. 1989; Bluetongue virus genetics and genome structure. Virus Research 13:179–206
    [Google Scholar]
  26. Roy P., Bishop D. H. L., Leblois H., Erasmus B. J. 1994a; Long-lasting protection of sheep against bluetongue challenge after vaccination with virus-like particles: evidence for homologous and partial heterologous protection. Vaccine 12:805–811
    [Google Scholar]
  27. Roy P., Callis J., Erasmus B. J. 1994b; Protection of sheep against bluetongue disease after vaccination with core-like and viruslike particles: evidence for homologous and partial heterologous protection. In Proceedings of the 27th Annual Meeting, USA Health Association pp 88–97 23–29th October 1993 Las Vegas, Nevada
    [Google Scholar]
  28. Sambrook J., Maniatis T., Fritsch E. F. 1989 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  29. Shiu S. Y. W., Reid H. W., Gould E. A. 1992; Louping ill virus envelope protein expressed by recombinant baculovirus and vaccinia virus fails to stimulate protective immunity. Virus Research 26:213–229
    [Google Scholar]
  30. Stott J. L., Osburn B. L., Barber T. L., Sawyer M. 1979; Immunological response of sheep to an experimental inactivated BTV vaccine. Journal of the American Veterinary and Medical Association 175:611–615
    [Google Scholar]
  31. Stott J. L., Barber T. L., Osburn B. I. 1985a; Immunologic response of sheep to inactivated and virulent bluetongue virus. American Journal of Veterinary Research 46:1043–1049
    [Google Scholar]
  32. Stott J. L., Osburn B. L., Bushnell R., Loomis E. C., Squire K. R. E. 1985b; Epizootiological study of bluetongue virus infection in California livestock: an overview. Progress in Clinical and Biological Research 178:571–582
    [Google Scholar]
  33. Takamatsu H., Jeggo M. H. 1989; Cultivation of bluetongue virus-specific ovine T cells and their cross-reactivity with different serotype viruses. Immunology 66:258–263
    [Google Scholar]
  34. Tewari D., Whalley J. M., Love D. N., Field H. J. 1994; Characterization of immune responses to baculovirus-expressed equine herpesvirus type 1 glycoproteins D and H in a murine model. Journal of General Virology 75:1735–1741
    [Google Scholar]
  35. Townsend A., Bodmer H. 1989; Antigen recognition by class I-restricted T lymphocytes. Annual Review of Immunology 7:601–624
    [Google Scholar]
  36. Whitton J. L., Southern P. J., Oldstone M. B. A. 1988; Analyses of the cytotoxic T lymphocyte responses to glycoprotein and nucleoprotein components of lymphocytic choriomeningitis virus. Virology 162:321–327
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-5-997
Loading
/content/journal/jgv/10.1099/0022-1317-77-5-997
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error