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Volume 77, Issue 5

Immune cell infiltration in corneas of mice with recurrent herpes simplex virus disease Free

Abstract

Reactivation of latent herpes simplex virus type 1 (HSV-1) infection was induced by UV irradiation of the corneas of latently infected mice. On days 1–4 after stimulation, infectious virus was sought in nervous and ocular tissue. On days 4, 7 and 10, eyes with either recurrent epithelial or stromal disease and appropriate controls were stained to identify immune cells and HSV-1 antigens. The maximum incidence of infectious virus was on day 2 when 5/10 ophthalmic parts of the trigeminal ganglion yielded HSV. Thus in this mouse model, as in humans, reactivation of virus in the trigeminal ganglion is the likely source of virus producing recurrent disease and shedding in the tear film. On day 4, when virus antigens were still present, granulocytes were the predominant infiltrating cell in corneas with either type of disease. Small numbers of T cells, dendritic cells and cells expressing MHC class II were also present. In stromal disease, the granulocyte infiltrate persisted and T cells remained sparse. In contrast, in epithelial disease, granulocyte numbers rapidly declined and both CD4 and CD8 T cells (present at a ratio of 1:1) increased significantly. The secondary immune response to virus antigen is more rapid and vigorous than that during primary corneal infection. Granulocytes may play a role in the initial clearance of virus, however, the other types of cells present early on provide the potential for a local secondary immune response. The high proportion of CD8 cells in epithelial disease compared with stromal disease suggests that they may be acting as suppressors.

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© Journal of General Virology 1996
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1996-05-01
2024-04-24
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References

  1. Cleator G. M., Klapper P. E., Dennett C., Sullivan A. L., Bonshek R. E., Marcyniuk B., Tullo A. B. 1994; Corneal donor infection by herpes simplex virus: herpes simplex virus DNA in donor corneas. Cornea 13:294–304
     [Google Scholar]
  2. Cook S. D., Hill J. M., Lynas C., Maitland N. J. 1991; Latency-associated transcripts in corneas and ganglia of HSV-1 infected rabbits. British Journal of Ophthalmology 33:644–648
     [Google Scholar]
  3. Crowle A. J. 1975; Delayed hypersensitivity in the mouse. Advances in Immunology 20:197–264
     [Google Scholar]
  4. Doymaz M. Z., Rouse B. T. 1992; Immunopathology of herpes simplex virus infections. Current Topics in Microbiology and Immunology 179:21–136
     [Google Scholar]
  5. Easty D. L., Shimeld C., Claoue C. M. P., Menage M. 1987; Herpes simplex virus isolation in chronic stromal keratitis: human and laboratory studies. Current Eye Research 6:69–74
     [Google Scholar]
  6. Ghiasi H., Wechsler S. L., Kaiwar R., Nesburn A. B., Hoeman F. M. 1995; Local expression of tumour necrosis factor alpha and interleukin-2 correlates with protection against corneal scarring after ocular challenge of vaccinated mice with herpes simplex virus type 1. Journal of Virology 69:334–340
     [Google Scholar]
  7. Girolomoni G., Tigelaar R. E. 1990; Capsaicin-sensitive primary sensory neurons are potent modulators of murine delayed-type hypersensitivity reactions. Journal of Immunology 145:1105–1112
     [Google Scholar]
  8. Harbour D. A., Hill T. J., Blyth W. A. 1983; Recurrent herpes simplex in the mouse: inflammation in the skin and activation of virus in the ganglia following peripheral stimulation. Journal of General Virology 64:1491–1498
     [Google Scholar]
  9. Heiligenhaus A., Berra A., Foster C. S. 1994; CD4 + V/18+ T cells mediate herpes stromal keratitis. Current Eye Research 13:711–716
     [Google Scholar]
  10. Kaye S. B., Lynas C., Patterson A., Risk A., McCarthy K., Hart C. A. 1991; Evidence for herpes simplex viral latency in the human cornea. British Journal of Ophthalmology 75:195–200
     [Google Scholar]
  11. Kerhl J. H., Wakefield L. M., Roberts A. B., Jakawlew S., Alvarez-Mon M., Derynck R., Sporn M. B., Fauci A. S. 1986; Production of TGF-/J by human T-lymphocytes and its potential role in the regulation of T-cell growth. Journal of Experimental Medicine 163:1037–1050
     [Google Scholar]
  12. Laycock K. A., Lee S. F., Brady R. H., Pepose J. S. 1991; Characterization of a murine model of recurrent herpes simplex viral keratitis induced by ultraviolet B radiation. Investigative Ophthalmology and Visual Science 32:2741–2746
     [Google Scholar]
  13. Mackay C. R., Marston W. L., Dudler L. 1990; Naive and memory T cells show distinct pathways of lymphocyte recirculation. Journal of Experimental Medicine 171:801–817
     [Google Scholar]
  14. Miller J. K., Laycock K. A., Nash M. M., Pepose J. S. 1993; Corneal Langerhans cell dynamics after herpes simplex virus reactivation. Investigative Ophthalmology and Visual Science 34:2282–2290
     [Google Scholar]
  15. Nicholls S. M., Shimeld C., Easty D. L., Hill T. J. 1996; Recurrent herpes simplex after corneal transplantation. Investigative Ophthalmology and Visual Science 37: in press
    [Google Scholar]
  16. O’Brien W. J., Taylor J. L. 1989; The isolation of herpes simplex virus from rabbit corneas during latency. Investigative Ophthalmology and Visual Science 30:357–364
     [Google Scholar]
  17. Opremcak E. M., Rice B. A., Wells P. A., Foster C. S. 1990; Histology and immunohistology of Igh-1 restricted herpes simplex keratitis in BALB/c congenic mice. Investigative Ophthalmology and Visual Science 31:305–312
     [Google Scholar]
  18. Russel R. G., Naisse M. P., Larsen H. S., Rouse B. T. 1984; Role of T-lymphocytes in the pathogenesis of herpetic stromal keratitis. Investigative Ophthalmology and Visual Science 25:938–944
     [Google Scholar]
  19. Shimeld C., Hill T. J., Blyth B., Easty D. L. 1989; An improved model of recurrent herpetic eye disease in mice. Current Eye Research 8:1193–1205
     [Google Scholar]
  20. Shimeld C., Hill T. J., Blyth W. A., Easty D. L. 1990a; Reactivation of latent infection and induction of recurrent eye disease in mice. Journal of General Virology 71:397–404
     [Google Scholar]
  21. Shimeld C., Hill T. J., Blyth W. A., Easty D. L. 1990b; Passive immunization protects the mouse eye from damage after herpes simplex virus infection by limiting spread of virus in the nervous system. Journal of General Virology 71:681–687
     [Google Scholar]
  22. Shimeld C., Whiteland J. L., Nicholls S. M., Grinfeld E., Easty D. L., Gao H., Hill T. J. 1995; Immune cell infiltration and persistence in the mouse trigeminal ganglion after infection of the cornea with herpes simplex type 1. Journal of Neuroimmunology 61:7–16
     [Google Scholar]
  23. Snedecar G. W., Cochran W. G. 1967; Statistical Methods, 6th edn. Ames, Iowa: Iowa State University Press;
     [Google Scholar]
  24. Springer T. 1990; Adhesion receptors of the immune system. Nature 346:425–434
     [Google Scholar]
  25. Tullo A. B., Shimeld C., Blyth W. A., Hill T. J., Easty D. L. 1982; Spread of virus and distribution of latent infection following ocular herpes simplex in the non-immune and immune mouse. Journal of General Virology 63:95–101
     [Google Scholar]
  26. Tullo A. B., Shimeld C., Blyth W. A., Hill T. J., Easty D. L. 1983; Ocular infection with herpes simplex virus in nonimmune and immune mice. Archives of Ophthalmology 101:961–964
     [Google Scholar]
  27. Wang H., Sheu M., Stulting R. D., Kaplan H. J. 1988; Immunohistochemical evaluation of murine HSV-1 keratouveitis. Current Eye Research 8:37–46
     [Google Scholar]
  28. Whiteland J. L., Nicholls S. M., Shimeld C., Easty D. L., Williams N. A., Hill T. I. 1995; Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. Journal of Histochemistry and Cytochemistry 43:313–320
     [Google Scholar]
  29. Williams L. E., Nesburn A. B., Kaufman H. E. 1965; Experimental induction of disciform keratitis. Archives of Ophthalmology 73:112–114
     [Google Scholar]
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Immune cell infiltration in corneas of mice with recurrent herpes simplex virus disease
J. Gen. Virol. 77, 977 (1996); https://doi.org/10.1099/0022-1317-77-5-977
/content/journal/jgv/10.1099/0022-1317-77-5-977
/content/journal/jgv/10.1099/0022-1317-77-5-977
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