RT Journal Article SR Electronic(1) A1 Britton, Paul A1 Green, Philip A1 Kottier, Sanneke A1 Mawditt, Karen L. A1 Penzes, Zoltan A1 Cavanagh, David A1 Skinner, Michael A.YR 1996 T1 Expression of bacteriophage T7 RNA polymerase in avian and mammalian cells by a recombinant fowlpox virus JF Journal of General Virology, VO 77 IS 5 SP 963 OP 967 DO https://doi.org/10.1099/0022-1317-77-5-963 PB Microbiology Society, SN 1465-2099, AB The bacteriophage T7 RNA polymerase gene was integrated into the fowlpox virus genome under the control of the vaccinia virus early/late promoter, P7.5. The recombinant fowlpox virus, fpEFLT7pol, stably expressed T7 RNA polymerase in avian and mammalian cells, allowing transient expression of transfected genes under the control of the T7 promoter. The recombinant fowlpox virus expressing T7 RNA polymerase offers an alternative to the widely used vaccinia virus vTF7-3, or the recently developed modified vaccinia virus Ankara (MVA) T7 RNA polymerase recombinant, a highly attenuated strain with restricted host-range. Recombinant fowlpox viruses have the advantage that as no infectious virus are produced from mammalian cells they do not have to be used under stringent microbiological safety conditions., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-77-5-963