@article{mbs:/content/journal/jgv/10.1099/0022-1317-77-5-953, author = "Haig, D. McK. and Hutchison, G. and Thomson, J. and Yirrell, D. and Reid, H. W.", title = "Cytolytic activity and associated serine protease expression by skin and afferent lymph CD8+ T cells during orf virus reinfection", journal= "Journal of General Virology", year = "1996", volume = "77", number = "5", pages = "953-961", doi = "https://doi.org/10.1099/0022-1317-77-5-953", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-77-5-953", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Following orf virus reinfection in sheep, CD8+ T cells were recruited to the lesion site in the skin during the period of viral replication and appeared in increased numbers in afferent lymph draining the infection site. A proportion of the CD8+ T cells were activated, particularly in the skin, as determined by their expression of the cytolytic cell-associated serine protease, BLT-esterase. This was a useful marker for activated cytolytic cells as there was a good correlation between afferent lymph CD8+ T cell-mediated lysis of autologous orf virus-infected skin cells and their expression of BLT-esterase in vitro. The majority of the cells that expressed BLT-esterase in the skin and lymph were CD8+ T cells and cytolysis in vitro was predominately by the MHC class I antigen presentation pathway. The conclusion is that orf virus reinfection is characterized by limited viral replication and stimulation of an activated CTL response in the skin.", }