1887

Abstract

Regions of the genome of the multinucleocapsid nuclear polyhedrosis virus (OpMNPV) containing the DNA polymerase and helicase genes were sequenced. The DNA polymerase and helicase genes encode predicted proteins of 985 (112.6 kDa) and 1223 (140.5 kDa) amino acids and exhibited 63% and 59% amino acid identity, respectively, with their homologues in the MNPV (AcMNPV). The influence of sequence variation between the OpMNPV and AcMNPV DNA polymerase and helicase was investigated by employing gene substitution experiments in transient replication assays in and cells. The DNA polymerase gene appeared to be interchangeable in this assay; both the AcMNPV and OpMNPV DNA polymerase supported high levels of replication of an origin-containing reporter plasmid when substituted for their homologue and cotransfected with a set of heterologous essential and stimulatory replication genes into uninfected insect cells. However, the OpMNPV helicase failed to support replication when it replaced the AcMNPV helicase from the set of AcMNPV replication genes cotransfected into cells. In contrast, the AcMNPV helicase gene supported about 50% of the level of replication when substituted for its homologue in the OpMNPV set of replication genes.

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1996-05-01
2022-01-25
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References

  1. Ahrens C. H., Rohrmann G. F. 1995a; Identification of essential trans-acting regions required for DNA replication of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus: lef-1 is an essential replication gene. Virology 207:417–428
    [Google Scholar]
  2. Ahrens C. H., Rohrmann G. F. 1995b; Replication of Orgyia pseudotsugata baculovirus DNA: ie-1 and lef-2 are essential and ie- 2, p34 and Op-iap are stimulatory genes. Virology 212:650–662
    [Google Scholar]
  3. Ahrens C. H., Carlson C., Rohrmann G. F. 1995a; Identification, sequence and transcriptional analysis of lef-3, a gene essential for Orgyia pseudotsugata baculovirus DNA replication. Virology 210:372–382
    [Google Scholar]
  4. Ahrens C. H., Pearson M. N., Rohrmann G. F. 1995b; Identification and characterization of a second putative origin of DNA replication in a baculovirus of Orgyia pseudotsugata . Virology 207:572–576
    [Google Scholar]
  5. Argos P. 1988; A sequence motif in many polymerases. Nucleic Acids Research 16:9909–9916
    [Google Scholar]
  6. Ayres M. D., Howard S. C., Kuzio J., Lopez-Ferber M., Possee R. D. 1994; The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202:586–605
    [Google Scholar]
  7. Birnbaum M. J., Clem R. J., Miller L. K. 1994; An apoptosis-inhibiting gene from a nuclear polyhedrosis virus encoding a polypeptide with Cys/His sequence motifs. Journal of Virology 68:2521–2528
    [Google Scholar]
  8. Bjornson R. M., Glocker B., Rohrmann G. F. 1992; Characterization of the nucleotide sequence of the Lymantria dispar nuclear polyhedrosis virus DNA polymerase gene region. Journal of General Virology 73:3177–3183
    [Google Scholar]
  9. Blissard G. W., Rohrmann G. F. 1990; Baculovirus diversity and molecular biology. Annual Review of Entomology 35:127–155
    [Google Scholar]
  10. Chaeychomsri S., Ikeda M., Kobayashi M. 1995; Nucleotide sequence and transcriptional analysis of the DNA polymerase gene of Bombyx mori nuclear polyhedrosis virus. Virology 206:435–447
    [Google Scholar]
  11. Clem R. J., Fechheimer M., Miller L. K. 1991; Prevention of apoptosis by a baculovirus gene during infection of insect cells. Science 254:1388–1390
    [Google Scholar]
  12. Cowan P., Bulach D., Goodge K., Robertson A., Tribe D. E. 1994; Nucleotide sequence of the polyhedrin gene region of Helicoverpa zea single nucleocapsid nuclear polyhedrosis virus: placement of the virus in lepidopteran nuclear polyhedrosis virus group II. Journal of General Virology 75:3211–3218
    [Google Scholar]
  13. Croizier G., Croizier L., Argaud O., Poudevigne D. 1994; Extension of Autographa californica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the pl 43 helicase gene. Proceedings of the National Academy of Sciences, USA 91:48–52
    [Google Scholar]
  14. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  15. Felsenstein J. 1989; PHYLIP – phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  16. Gombart A. F., Blissard G. W., Rohrmann G. F. 1989; Characterization of the genetic organization of the Hin dIII-M region of the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata reveals major differences among baculoviruses. Journal of General Virology 70:1815–1828
    [Google Scholar]
  17. Gorbalenya A. E., Koonin E. V., Donchenko A. P., Blinov V. M. 1989a; One more conserved sequence motif in helicases. Nucleic Acids Research 16:7734
    [Google Scholar]
  18. Gorbalenya A. E., Koonin E. V., Donchenko A. P., Blinov V. M. 1989b; Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. Nucleic Acids Research 17:4713–4730
    [Google Scholar]
  19. Gross C. H., Wolgamot G. M., Russell R. L. Q., Pearson M. N., Rohrmann G. F. 1993; A 37 kDa glycoprotein from a baculovirus of Orgyia pseudotsugata is localized to cytoplasmic inclusion bodies. Journal of Virology 67:469–475
    [Google Scholar]
  20. Henikoff S. 1987; Unidirectional digestion with exonuclease III in DNA sequence analysis. Methods in Enzymology 155:156–165
    [Google Scholar]
  21. Henikoff S., Henikoff J. G. 1991; Automated assembly of protein blocks for database searching. Nucleic Acids Research 19:6565–6572
    [Google Scholar]
  22. Hodgman T. C. 1988; A new superfamily of replicative proteins. Nature 333:22–23 (Author’s correction: Nature 333, 578.)
    [Google Scholar]
  23. Hwang C. B. C., Ruffner K. L., Coen D. M. 1992; A point mutation within a distinct conserved region of the herpes simplex virus DNA polymerase gene confers drug resistance. Journal of Virology 66:1774–1776
    [Google Scholar]
  24. Jehle J. A., Backhaus H. 1994; Genome organization of the DNA-binding protein gene region of Cryptophlebia leucotreta granulosis virus is closely related to that of nuclear polyhedrosis viruses. Journal of General Virology 75:1815–1820
    [Google Scholar]
  25. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;
    [Google Scholar]
  26. Kool M., Voeten J. T. M., Goldbach R. W., Tramper J., Vlak J. M. 1993; Identification of seven putative origins of Autographa californica MNPV DNA replication. Journal of General Virology 74:2661–2668
    [Google Scholar]
  27. Kool M., Ahrens C., Goldbach R. W., Rohrmann G. F., Vlak J. M. 1994; Identification of genes involved in DNA replication of the Autographa californica baculovirus. Proceedings of the National Academy of Sciences, USA 91:11212–11216
    [Google Scholar]
  28. Leisy D. J., Rohrmann G. F. 1993; Characterization of the replication of plasmids containing hr sequences in baculovirus-infected Spodoptera frugiperda cells. Virology 196:722–730
    [Google Scholar]
  29. Leisy D. J., Rohrmann G. F., Beaudreau G. S. 1984; Conservation of genome organization in two multicapsid nuclear polyhedrosis viruses. Journal of Virology 52:699–702
    [Google Scholar]
  30. Linder P., Lasko P. F., Ashburner M., Leroy P., Nielsen P. J., Nishi K., Schnier J., Slonimski P. P. 1989; Birth of the D-E-A-D box. Nature 337:121–122
    [Google Scholar]
  31. Lipman D. J., Altschul F. A., Kececioglu J. D. 1989; A tool for multiple sequence alignment. Proceedings of the National Academy of Sciences, USA 86:4412–4415
    [Google Scholar]
  32. LIu J. J., Carstens E. B. 1995; Identification, localization, transcription, and sequence analysis of the Choristoneura fumiferana nuclear polyhedrosis virus DNA polymerase gene. Virology 209:538–549
    [Google Scholar]
  33. Lu A., Carstens E. B. 1991; Nucleotide sequence of a gene essential for viral DNA replication in the baculovirus Autographa californica nuclear polyhedrosis virus. Virology 181:336–347
    [Google Scholar]
  34. Lu A., Miller L. K. 1995; The roles of eighteen baculovirus late expression factor genes in transcription and DNA replication. Journal of Virology 69:975–982
    [Google Scholar]
  35. Maeda S., Kamita S. G., Kondo A. 1993; Host range expansion of Autographa californica nuclear polyhedrosis virus (NPV) following recombination of a 0.6-kilobase-pair DNA fragment originating from Bombyx mori NPV. Journal of Virology 67:6234–6238
    [Google Scholar]
  36. Matson S., Kaiser-Rogers A. 1990; DNA helicases. Annual Review of Biochemistry 59:289–329
    [Google Scholar]
  37. Morrison A., Bell J. B., Kunkel T. A., Sugino A. 1991; Eukaryotic DNA polymerase amino acid sequence required for 3′→5′ exonuclease activity. Proceedings of the National Academy of Sciences, USA 88:9473–9477
    [Google Scholar]
  38. Ohresser M., Morin N., Cerutti M., Delsert C. 1994; Temporal regulation of a complex and unconventional promoter by viral products. Journal of Virology 68:2589–2597
    [Google Scholar]
  39. Olsen S. 1990; Phylogeny reconstruction. In Molecular Systematics Chapter 11 Edited by Hillis D. M., Moritz C. Sunderland, Mass.: Sinauer Associates;
    [Google Scholar]
  40. Pearson M. N., Rohrmann G. F. 1995; Lymantria dispar nuclear polyhedrosis virus homologous regions: characterization of their ability to function as replication origins. Journal of Virology 69:213–221
    [Google Scholar]
  41. Pearson M. N., Bjornson R. M., Pearson G. D., Rohrmann G. F. 1992; The Autographa californica baculovirus genome: evidence for multiple replication origins. Science 257:1382–1384
    [Google Scholar]
  42. Pearson M. N., Bjornson R. M., Ahrens C., Rohrmann G. F. 1993; Identification and characterization of a putative origin of DNA replication in the genome of a baculovirus pathogenic for Orgyia pseudotsugata . Virology 197:715–725
    [Google Scholar]
  43. Russell R. L. Q., Rohrmann G. F. 1993; A 25 kilodalton protein is associated with the envelopes of occluded baculovirus virions. Virology 195:532–540
    [Google Scholar]
  44. Saraste M., Sibbald P. R., Wittinghofer A. 1990; The P-loop – a common motif in ATP- and GTP-binding proteins. Trends in Biological Sciences 15:430–434
    [Google Scholar]
  45. Summers M. D., Smith G. E. 1987 A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures Texas Agricultural Experiment Station Bulletin no. 1555
    [Google Scholar]
  46. Theilmann D. A., Stewart S. 1991; Identification and characterization of the IE-1 gene of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 180:492–508
    [Google Scholar]
  47. Tomalski M. D., Wu J., Miller L. K. 1988; The location, sequence, transcription, and regulation of a baculovirus DNA polymerase gene. Virology 167:591–600
    [Google Scholar]
  48. Walker J. E., Saraste M., Rushwick M. J., Gay N. J. 1982; Distantly related sequences in the a-and b-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO Journal 1:945–951
    [Google Scholar]
  49. Wang T. S.-F. 1991; Eukaryotic DNA polymerases. Annual Review of Biochemistry 60:513–552
    [Google Scholar]
  50. Zhu L., Weller S. K. 1992; The six conserved helicase motifs of the UL5 gene product, a component of the herpes simplex virus type 1 helicase-primase, are essential for its function. Journal of Virology 66:469–479
    [Google Scholar]
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