1887

Abstract

Methylamine, a lysosomotropic alkalinizing agent, blocked mouse mammary tumour virus (MMTV) infection in normal mouse mammary epithelium, suggesting that internalization and acidification are necessary for cell penetration. This mechanism was further supported by the fact that intact MMTV induced the translocation of its cellular binding protein from the plasmalemma to the microsomes; however, isolated gp52, the MMTV envelope protein that binds this receptor, did not redistribute the binding protein. These data suggest that either another viral component, in addition to gp52, is needed for cell entry or that internalization requires receptor aggregation, which only the multivalent viral envelope can induce.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-4-793
1996-04-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/4/JV0770040793.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-4-793&mimeType=html&fmt=ahah

References

  1. Andersen K. B. 1994; A domain of murine retrovirus surface protein gp70 mediates cell fusion, as shown in a novel SC-1 cell fusion system. Journal of Virology 68:3175–3182
    [Google Scholar]
  2. Bolander F. F. 1993a; Partial purification and characterization of a cellular binding protein for the mouse mammary tumor virus. Endocrine 1:19–23
    [Google Scholar]
  3. Bolander F. F. 1993b; Effect of hormones on the infectivity of the mouse mammary tumor virus. Experimental and Clinical Endocrinology 12:53–56
    [Google Scholar]
  4. Bolander F. F. 1994; Regulation of the mouse mammary tumor virus receptor by phosphorylation and internalization in mammary epithelial cells. Journal of Cellular Physiology 161:124–128
    [Google Scholar]
  5. Bolander F. F., Blackstone M. E. 1990; Developmental and hormonal regulation of a mouse mammary tumour virus glycoprotein in normal mouse mammary epithelium. Journal of Molecular Endocrinology 4:101–106
    [Google Scholar]
  6. Bolander F. F., Blackstone M. E. 1991; Tissue distribution of the cellular binding protein for the mouse mammary tumour virus. Journal of Molecular Endocrinology 7:169–174
    [Google Scholar]
  7. Brunette D. M., Till J. E. 1971; A rapid method for the isolation of Lcell surface membranes using an aqueous two-phase polymer system. Journal of Membrane Biology 5:215–224
    [Google Scholar]
  8. Compton T., Nowlin D., Cooper N. R. 1993; Initiation of human cytomegalovirus infection requires initial interaction with cell surface heparin sulfate. Virology 193:834–841
    [Google Scholar]
  9. Dickson C., Peters G. 1983; Proteins encoded by mouse mammary tumour virus. Current Topics in Microbiology and Immunology 106:1–34
    [Google Scholar]
  10. Edery M., Pang K., Larson L., Nandi S. 1989; Turnover of epidermal growth factor binding sites in mouse mammary epithelial cells. Biomedicine and Pharmacotherapy 43:361–368
    [Google Scholar]
  11. Fitzgerald D. K., Colvin B., Maw al R., Ebner K. E. 1970; Enzymatic assay for galactosyl transferase activity of lactose synthetase and a-lactalbumin in purified and crude systems. Analytical Biochemistry 36:43–61
    [Google Scholar]
  12. Juergens W. G., Stockdale F. E., Topper Y. J., Elias J. J. 1965; Hormonal-dependent differentiation of mouse mammary gland in vitro. Proceedings of the National Academy of Sciences, USA 54:629–634
    [Google Scholar]
  13. Houdebine L. M. 1980; Effect of various lysosomotropic agents and microtubule disrupting drugs on the lactogenic and the mammogenic action of prolactin. European Journal of Cell Biology 22:755–760
    [Google Scholar]
  14. Karger A., Mettenleiter T. C. 1993; Glycoproteins gill and gp50 play dominant roles in the biphasic attachment of pseudorabies virus. Virology 194:654–664
    [Google Scholar]
  15. McClain D., Fuller A. O. 1994; Cell-specific kinetics and efficiency of herpes simplex virus type I entry are determined by two distinct phases of attachment. Virology 198:690–702
    [Google Scholar]
  16. Marsh M., Pelchen-Matthews A. 1993; Entry of animal viruses into cells. Reviews in Medical Virology 3:173–185
    [Google Scholar]
  17. Mohammadi M., Honegger A., Sorokin A., Ullrich A., Schlessinger J., Hurwitz D. R. 1993; Aggregation-induced activation of the epidermal growth factor receptor protein tyrosine kinase. Biochemistry 32:8742–8748
    [Google Scholar]
  18. Munoz B., Bolander F. F. 1989; Prolactin regulation of mouse mammary tumor virus (MMTV) expression in normal mouse mammary epithelium. Molecular and Cellular Endocrinology 62:23–29
    [Google Scholar]
  19. Ono M., Oka T. 1980; The differential actions of cortisol on the accumulation of α-lactalbumin and casein in midpregnant mouse mammary gland in culture. Cell 19:473–480
    [Google Scholar]
  20. Redmond S., Peters G., Dickson C. 1984; Mouse mammary tumor virus can mediate cell fusion at reduced pH. Virology 133:393–402
    [Google Scholar]
  21. Rut H., Lebrun J. J., Kirken R. A., Kelly P. A., Farrar W. L. 1994; JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization. Endocrinology 135:1299–1306
    [Google Scholar]
  22. Scatchard G. 1949; The attractions of proteins for small molecules and ions. Annals of the New York Academy of Sciences 51:660–672
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-4-793
Loading
/content/journal/jgv/10.1099/0022-1317-77-4-793
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error