1887

Abstract

We characterized the antigenic sites on the nucleocapsid protein (NP) of Hantaan virus (HTN) using 10 monoclonal antibodies (MAbs). At least seven antigenic sites were revealed by a competitive binding assay and divided into three partially overlapping antigenic regions (I, II and III). Regions I [amino acids (aa) 1–103], II (aa 104–204) and III (aa 205–402) were mapped on NP by examining the reactivity of truncated gene products. Those that corresponded to region I reacted with immune mouse serum, indicating that the region contained major linear epitopes as reported with Four corners virus (FCV) and Puumala virus (PUU) NP. At least one MAb to each region inhibited viral growth when they were introduced into cells by scrape-loading. In addition, they conferred protection from a lethal HTN challenge to newborn mice. A PEPSCAN assay localized the epitope of MAb E5/G6 between aa 166–175. Since E5/G6, which had the highest inhibitory effect both in cells and in mice, showed no virus neutralization activity by ordinary neutralization test, this region is suggested to be important for the virus growth after entry into the cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-4-695
1996-04-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/4/JV0770040695.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-4-695&mimeType=html&fmt=ahah

References

  1. Arikawa J., Takashima I., Hashimoto N. 1985; Cell fusion by haemorrhagic fever with renal syndrome (HFRS) viruses and its application for titration of virus infectivity and neutralizing antibody. Archives of Virology 86:303–313
    [Google Scholar]
  2. Arikawa J., Schmaljohn A. L., Dalrymple J. M., Schmaljohn C. S. 1989; Characterization of Hantaan virus envelope glycoprotein antigenic determinants defined by monoclonal antibodies. Journal of General Virology 70:615–624
    [Google Scholar]
  3. Arikawa J., Lapenotiere H. F., Iacono C.-L., Wang M. L., Schmaljohn C. S. 1990; Coding properties of the S and the M genome segments of Sapporo rat virus: comparison to other causative agents of hemorrhagic fever with renal syndrome. Virology 176:114–125
    [Google Scholar]
  4. Brummer-Korvenkontio M., Henittonen H., Vaheri A. 1982; Hemorrhagic fever with renal syndrome in Finland. Scandinavian Journal of Infectious Diseases 38:88–91
    [Google Scholar]
  5. Carrol A. R., Wagner R. R. 1978; Inhibition of transcription by immunogloblins directed against the ribonucleoprotein of homotypic and heterotypic vesicular stomatitis viruses. Journal of Virology 25:675–684
    [Google Scholar]
  6. Gott P., Stohwasser R., Schnitzler P., Darai G., Bautz E. K. 1993; RNA binding of recombinant nucleocapsid proteins of hantaviruses. Virology 194:332–337
    [Google Scholar]
  7. Harmon S. A., Summers D. F. 1982; Characterization of monospecific antisera against all five vesicular stomatitis virus-specific proteins: anti-L and anti-NS inhibit transcription in vitro. Virology 120:194–204
    [Google Scholar]
  8. Horiuchi T., Marumoto Y., Saeki Y., Sato Y., Furusawa M., Kondo A., Maeda S. 1987; High-level expression of the human-alpha-interferon gene through the use of an improved baculovirus vector in the silkworm, Bombyx mori . Agricultural and Biological Chemistry 51:1573–1580
    [Google Scholar]
  9. Isegawa Y., Tanishita O., Ueda S., Yamanishi K. 1994; Association of serine in position 1124 of Hantaan virus glycoprotein with virulence in mice. Journal of General Virology 75:3273–3278
    [Google Scholar]
  10. Jenison S., Yamada T., Morris C., Anderson B., Torrez M.-N., Keller N., Hjelle B. 1994; Characterization of human antibody responses to four corners hantavirus infections among patients with hantavirus pulmonary syndrome. Journal of Virology 68:3000–3006
    [Google Scholar]
  11. Kitamura T., Morita C., Komatsu T., Sugiyama K., Arikawa J., Shiga S., Takeda H., Akao Y., Imaizumi K., Oya A., Hashimoto N., Urasawa S. 1983; Isolation of virus causing hemorrhagic fever with renal syndrome (HFRS) through a cell culture system. Japanese Journal of Medical Science and Biology 36:17–25
    [Google Scholar]
  12. Lafon M., Lafage M. 1987; Antiviral activity of monoclonal antibodies specific for the internal proteins N and NS of rabies virus. Journal of General Virology 68:3113–3123
    [Google Scholar]
  13. Lee H. W., Lee P. W., Johnson K. M. 1978; Isolation of the etiologic agent of Korean hemorrhagic fever. Journal of Infectious Diseases 137:298–302
    [Google Scholar]
  14. Lee P. W., Amyx H. L., Yanagihara R., Gajdusek D. C., Goldgaber D., Gibbs C. J. J. 1982; Partial characterization of Prospect Hill virus isolated from meadow voles in the United States. Journal of Infectious Diseases 152:826–829
    [Google Scholar]
  15. Levine B., Hardwick J. M., Trapp B. D., Crawford T. O., Bollinger R. C., Griffin D. E. 1991; Antibody-mediated clearance of alphavirus infection from neurons. Science 254:856–860
    [Google Scholar]
  16. Lundkvist A., Fatouros A., Niklasson B. 1991; Antigenic variation of European haemorrhagic fever with renal syndrome virus strains characterized using bank vole monoclonal antibodies. Journal of General Virology 72:2097–2103
    [Google Scholar]
  17. Lundkvist A., Bjorsten S., Niklasson B., Ahlborg N. 1995; Mapping of the B-cell determinants in the nucleocapsid protein of Puumala virus: definition of epitopes specific for acute immunoglobulin G recognition in humans. Clinical and Diagnostic Laboratory Immunology 2:82–86
    [Google Scholar]
  18. Maeda S., Kawai T., Obinata M., Fujiwara H., Horiuchi T., Saeki Y., Sato Y., Furusawa M. 1985; Production of human alpha-interferon in silkworm using a baculovirus vector. Nature 315:592–594
    [Google Scholar]
  19. Parrington M. A., Kang C. Y. 1990; Nucleotide sequence analysis of the S genomic segment of Prospect Hill virus: comparison with the prototype Hantavirus. Virology 175:167–175
    [Google Scholar]
  20. Plyusnin A., Vapalahti O., Lankinen H., Lehvaslaiho H., Apekina N., Myasnikov Y., Kalliokokko H., Henttonen H., Lundkvist A., Brummer-Korvenkontio M., Gavrilovskaya I., Vaheri A. 1994; Tula virus: a newly detected hantavirus carried by European common voles. Journal of Virology 8:7833–7783
    [Google Scholar]
  21. Rossi C. A., Schmaljohn C. S., Meegan J. M., Leduc J. W. 1990; Diagnostic potential of a baculovirus-expressed nucleocapsid protein for hantavirus. Archives of Virology Supplementum 119–28
    [Google Scholar]
  22. Ruo S. L., Sanchez A., Elliott L. H., Brammer L. S., Mccormick J. B., Fisher H.-S. 1991; Monoclonal antibodies to three strains of hantaviruses: Hantaan, R22, and Puumala. Archives of Virology 119:1–11
    [Google Scholar]
  23. Schmaljohn C. S., Jennings G. B., Hay J., Dalrymple J. M. 1986; Coding strategy of the S genome segment of Hantaan virus. Virology 155:633–643
    [Google Scholar]
  24. Schmaljohn C. S., Chu Y. K., Schmaljohn A. L., Dalrymple J. M. 1990; Antigenic subunits of Hantaan virus expressed by baculovirus and vaccinia virus recombinants. Journal of Virology 64:3162–3170
    [Google Scholar]
  25. Spiropoulou C. F., Morzunov S., Feldmann H., Sanchez A., Peters C. J., Nichol S. T. 1994; Genome structure and variability of a virus causing hantavirus pulmonary syndrome. Virology 200:715–723
    [Google Scholar]
  26. Takita S.-Y., Fujii H., Mifune K., Ito Y., Hiraga M., Nishizono A., Mannen K., Minamoto N. 1993; Resistance of mice vaccinated with rabies virus internal structural proteins to lethal infection. Archives of Virology 132:51–65
    [Google Scholar]
  27. Tamura M., Asada H., Kondo K., Tanishita O., Kurata T., Yamanishi K. 1989; Pathogenesis of Hantaan virus in mice. Journal of General Virology 70:2897–2906
    [Google Scholar]
  28. Vapalahti O., Kallio H.-M., Salonen E. M., Brummer K.-M., Vaheri A. 1992; Cloning and sequencing of Puumala virus Sotkamo strain S and M RNA segments: evidence for strain variation in hantaviruses and expression of the nucleocapsid protein. Journal of General Virology 73:829–838
    [Google Scholar]
  29. Wang M., Rossi C., Schmaljohn C. S. 1993; Expression of nonconserved regions of the S genome segments of three hantaviruses: evaluation of the expressed polypeptides for diagnosis of haemorrhagic fever with renal syndrome. Journal of General Virology 74:1115–1124
    [Google Scholar]
  30. Yamada T., Hjelle B., Lanzi R., Morris C., Anderson B., Jenison S. 1995; Antibody responses to Four Corners hantavirus infections in the deer mouse (Peromyscus maniculatus): identification of an immunodominant region of the viral nucleocapsid protein. Journal of Virology 9:1939–1194
    [Google Scholar]
  31. Yoshimatsu K., Arikawa J., Kariwa H. 1993a; Application of a recombinant baculovirus expressing hantavirus nucleocapsid protein as a diagnostic antigen in IFA test: cross reactivities among 3 serotypes of hantavirus which causes hemorrhagic fever with renal syndrome (HFRS). Journal of Veterinary Medical Science 55:1047–1050
    [Google Scholar]
  32. Yoshimatsu K., Yoo Y. C., Yoshida R., Ishihara C., Azuma I., Arikawa J. 1993b; Protective immunity of Hantaan virus nucleocapsid and envelope protein studied using baculovirus-expressed proteins. Archives of Virology 130:365–376
    [Google Scholar]
  33. Yoshimatsu K., Arikawa J., Yoshida R., Li H., Yoo Y. C., Kariwa H., Hashimoto N., Kakinuma M., Nobunaga T., Azuma I. 1995; Production and application of recombinant hantavirus nucleocapsid protein expressed in silkworm larvae as a diagnostic antigen for laboratory rat. Laboratory Animal Science 45:647–651
    [Google Scholar]
  34. Zöller L., Yang S., Gott P., Bautz E. K., Darai G. 1993; Use of recombinant nucleocapsid proteins of the Hantaan and nephropathia epidemica serotypes of Hantaviruses as immunodiagnostic antigens. Journal of Medical Virology 39:200–207
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-4-695
Loading
/content/journal/jgv/10.1099/0022-1317-77-4-695
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error