1887

Abstract

Vaccinia virus recombinants expressing either wild-type or mutant forms of human respiratory syncytial (RS) virus (Long strain) fusion (F) glycoprotein were obtained. Proteolytic processing of the precursor, F, and cell surface transport of the F glycoprotein were unaffected in the recombinants, except in those that contained the replacement Phe → Ser at position 237 of the F subunit. In recombinants containing this mutation, either alone or in combination with others, the traffic of the F molecule was arrested at some intermediate step of its transport to the cell surface and, consequently, the endoproteolytic cleavage of the F precursor was inhibited. Immunofluorescence staining of infected cells and endoglycosidase H (Endo-H) sensitivity assays indicated that the arrest occurred before the mid-Golgi compartment. Dimerization and folding of the F protein were also affected by the Phe → Ser substitution. Other amino acid replacements at positions 236 or 237 of the F subunit had various effects upon F maturation. These results are discussed in terms of the maturation requirements for the RS virus F molecule.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-4-649
1996-04-01
2022-08-15
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/4/JV0770040649.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-4-649&mimeType=html&fmt=ahah

References

  1. Anderson K., Stott E. J., Wertz G. W. 1992; Intracellular processing of the human respiratory syncytial virus fusion glycoprotein: amino acid substitutions affecting folding, transport and cleavage. Journal of General Virology 73:1177–1188
    [Google Scholar]
  2. Arbiza J., Taylor G., Lopez J. A., Furze J., Wyld S., Whyte P., Stott E. J., Wertz G. W., Sullender W., Trudel M., Melero J. A. 1992; Characterization of two antigenic sites recognized by neutralizing monoclonal antibodies directed against the F glycoprotein of human respiratory syncytial virus. Journal of General Virology 73:2225–2234
    [Google Scholar]
  3. Arumugham R. G., Hildreth S. W., Paradiso P. R. 1989a; Fatty acid acylation of the fusion glycoprotein of human respiratory syncytial virus. Journal of Biological Chemistry 264:10339–10342
    [Google Scholar]
  4. Arumugham R. G., Hildreth S. W., Paradiso P. R. 1989b; Interprotein disulfide bonding between F and G glycoproteins of human respiratory syncytial virus. Archives of Virology 105:65–79
    [Google Scholar]
  5. Bordo D., Argos P. 1991; Suggestions for ‘safe’ residue substitutions in site-directed mutagenesis. Journal of Molecular Biology 217:721–729
    [Google Scholar]
  6. Bourgeois C., Covaisier C., Bour J. B., Kohli E., Pothier P. 1991; Use of synthetic peptides to locate neutralizing antigenic domains on the fusion protein of respiratory syncytial virus. Journal of General Virology 72:1051–1058
    [Google Scholar]
  7. Cash P., Wunner W. H., Pringle C. R. 1977; A comparison of the polypeptides of human and bovine respiratory syncytial virus and murine pneumonia virus. Virology 82:369–379
    [Google Scholar]
  8. Chakrabarti S., Brechling K., Moss B. 1985; Vaccinia virus expression vector: coexpression of β-galactosidase provides visual screening of recombinant virus plaques. Molecular and Cellular Biology 5:3403–3409
    [Google Scholar]
  9. Chambers P., Pringle C. R., Easton A. J. 1992; Sequence analysis of the gene encoding the fusion glycoprotein of pneumonia virus of mice suggests possible conserved secondary structure elements in paramyxovirus fusion glycoproteins. Journal of General 73:1717–1724
    [Google Scholar]
  10. Collins P. L. 1991; The molecular biology of human respiratory syncytial virus (RSV) of the genus Pneumovirus. In The Paramyxoviruses pp 103–162 Edited by Kingsbury D. W. New York: Plenum Press;
    [Google Scholar]
  11. Collins P. L., Mottet G. 1991; Post-translational processing and oligomerization of the fusion glycoprotein of human respiratory syncytial virus. Journal of General Virology 72:3095–3101
    [Google Scholar]
  12. Cristina J., López J. A., Albo C., García-Barreno B., García J., Melero J. A., Portela A. 1990; Analysis of genetic variability in human respiratory syncytial virus by the RNase A mismatch cleavage method: subtype divergence and heterogeneity. Virology 174:126–134
    [Google Scholar]
  13. Einfeld D., Hunter E. 1991; Transport of membrane proteins to the cell surface. Current Topics in Microbiology and Immunology 170:107–139
    [Google Scholar]
  14. Fernie B. F., Dapolito G., Cote P. J. Jr, Gerin J. L. 1985; Kinetics of synthesis of respiratory syncytial virus glycoproteins. Journal of General Virology 66:1983–1990
    [Google Scholar]
  15. Fuerst T. R., Niles E. G., Studier F. W., Moss B. 1986; Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proceedings of the National Academy of Sciences, USA 83:8122–8126
    [Google Scholar]
  16. García-Barreno B., Jorcano J. L., Aukenbauer C., López-Galindez C., Melero J. A. 1988; Participation of cytoskeletal intermediate filaments in the infectious cycle of human respiratory syncytial virus (RSV). Virus Research 9:307–322
    [Google Scholar]
  17. García-Barreno B., Palomo C., Penas C., Delgado T., Perez-Brena P., Melero J. A. 1989; Marked differences in the antigenic structure of human respiratory syncytial virus F and G glycoproteins. Journal of Virology 63:925–932
    [Google Scholar]
  18. Garten W., Will C., Buckard K., Kuroda K., Ortmann D., Munk , Scholtissek C., Schnittler H., Drenckhahn D., Klenk H.-D. 1991; Structure and assembly of hemagglutinin mutants of fowl plague virus with impaired surface transport. Journal of Virology 66:1495–1505
    [Google Scholar]
  19. Gething M.-J., Sambrook J. 1992; Protein folding in the cell. Nature 355:33–45
    [Google Scholar]
  20. Gething M.-J., McCammon K., Sambrook J. 1989; Expression of wild-type and mutant forms of influenza hemagglutinin: the role of folding in intracellular transport. Cell 46:939–950
    [Google Scholar]
  21. Gruber C., Levine S. 1985a; Respiratory syncytial virus polypeptides. I V. The oligosaccharides of the glycoproteins. Journal of General Virology 66:825–832
    [Google Scholar]
  22. Gruber C., Levine S. 1985b; Respiratory syncytial virus polypeptides. V. Kinetics of glycoprotein synthesis. Journal of General Virology 66:1241–1247
    [Google Scholar]
  23. Higuchi R., Krummel B., Saiki R. K. 1988; A general method for ‘in vitro’ preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Research 16:7351–7367
    [Google Scholar]
  24. Johnson P. R., Collins P. L. 1988; The fusion glycoproteins of human respiratory syncytial virus of subgroups A and B: sequence conservation provides a structural basis for antigenic relatedness. Journal of General Virology 69:2623–2628
    [Google Scholar]
  25. Lerch R. A., Anderson K., Wertz G. W. 1991; Nucleotide sequence analysis of the bovine respiratory syncytial virus fusion protein mRNA and expression from a recombinant vaccinia virus. Virology 181:118–131
    [Google Scholar]
  26. López J. A., Villanueva N., Melero J. A., Portela A. 1988; Nucleotide sequence of the fusion and phosphoprotein genes of human respiratory syncytial (RS) virus Long strain: evidence for subtype genetic heterogeneity. Virus Research 10:249–262
    [Google Scholar]
  27. López J. A., Peñas C., García-Barreno B., Melero J. A., Portela A. 1990; Location of a highly conserved neutralizing epitope in the F glycoprotein of human respiratory syncytial virus. Journal of Virology 64:927–930
    [Google Scholar]
  28. López J. A., Andrew D., Carreno C., Whyte P., Taylor G., Melero J. A. 1993; Conformational constraints of conserved neutralizing epitopes from a major antigenic area of human respiratory syncytial virus fusion (F) glycoprotein. Journal of General Virology 74:2567–2577
    [Google Scholar]
  29. Maddon P. J., Dalgleish A. G., McDougal J. S., Clapham P. R., Weiss R. A., Axel R. 1986; The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and brain. Cell 47:333–342
    [Google Scholar]
  30. Martín-Gallardo A., Fien K. A., Hu B. T., Farley J. F., Seid R., Collins P. L., Hildreth S. W., Paradiso P. R. 1991; Expression of the F glycoprotein gene from human respiratory syncytial virus in Escherichia coli: mapping of a fusion inhibiting epitope. Virology 184:428–432
    [Google Scholar]
  31. Mathelse J. P., Walravens K., Collard A., Coppe P., Letesson J. J. 1995; Antigenic analysis of the F protein of the bovine respiratory syncytial virus: identification of two distinct antigenic sites involved in fusion inhibition. Archives of Virology 140:993–1005
    [Google Scholar]
  32. Morrison T., Portner A. 1991; Structure, function and intracellular processing of the glycoproteins of Paramyxoviridae. In The Paramyxoviruses pp 347–382 Edited by Kingsbury D. W. New York: Plenum Press;
    [Google Scholar]
  33. Olmsted R. A., Elango N., Prince G. A., Murphy B. R., Johnson P. R., Moss B., Chanock R. M., Collins P. L. 1986; Expression of the F glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the F and G glycoproteins to host immunity. Proceedings of the National Academy of Sciences, USA 83:7462–7466
    [Google Scholar]
  34. Parks G. D., Lamb R. A. 1990; Defective assembly and intracellular transport of mutant paramyxovirus hemagglutinin-neuraminidase proteins containing altered cytoplasmic domains. Journal of Virology 64:3605–3616
    [Google Scholar]
  35. Pastey M. K., Samal S. K. 1993; Structure and sequence comparison of bovine respiratory syncytial virus fusion protein. Virus Research 29:195–202
    [Google Scholar]
  36. Portela A., Rodriguez J. R., Rodriguez D., Rodriguez F., García-Barreno B., Melero J. A., Esteban M. 1989; Expression of the fusion (F) protein of human respiratory syncytial virus using an attenuated strain of vaccinia virus. In Genetics and Pathogenicity of Negative Strand Viruses pp 74–79 Edited by Kolakofsky D., Mahy B. W. J. Amsterdam: Elsevier/North-Holland Publishing;
    [Google Scholar]
  37. Qadri I., Gimeno C., Navarro D., Pereira L. 1991; Mutations in conformation-dependent domains of herpes simplex virus 1 glycoprotein B affect the antigenic properties, dimerization, and transport of the molecule. Virology 180:136–152
    [Google Scholar]
  38. Rueda P., García-Barreno B., Melero J. A. 1994; Loss of conserved cysteine residues in the attachment (G) glycoprotein of two human respiratory syncytial virus escape mutants that contain multiple A-G substitutions (hypermutations). Virology 198:653–662
    [Google Scholar]
  39. Satake M., Coligan J. E., Elango N., Norrby E., Venkatesan S. 1985; Respiratory syncytial virus envelope glycoprotein (G) has a novel structure. Nucleic Acids Research 13:7795–7812
    [Google Scholar]
  40. Stott E. J., Taylor G., Ball L. A., Anderson K., Young K. K.-Y., King A. M. Q., Wertz G. W. 1987; Immune and histopathological responses in animals vaccinated with recombinant vaccinia viruses that express individual genes of human respiratory syncytial virus. Journal of Virology 61:3855–3861
    [Google Scholar]
  41. Taylor G., Stott E. J., Bew M., Fernie B. F., Cote P. J., Collins A. P., Hughes M., Jebbett J. 1984; Monoclonal antibodies protect against respiratory syncytial virus infection in mice. Immunology 52:137–142
    [Google Scholar]
  42. Taylor G., Stott E. J., Furze J., Ford J., Sopp P. 1992; Protective epitopes on the fusion protein of respiratory syncytial virus recognized by murine and bovine monoclonal antibodies. Journal of General Virology 73:2217–2223
    [Google Scholar]
  43. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, USA 76:4350–4354
    [Google Scholar]
  44. Trudel M., Nadon F., Seguin C., Dionne G., Lacroix M. 1987; Identification of a synthetic peptide as part of a major neutralizing epitope of respiratory syncytial virus. Journal of General Virology 68:2273–2280
    [Google Scholar]
  45. Walravens K., Kettmann R., Collard A., Coppe P., Burny A. 1990; Sequence comparison between the fusion protein of human and bovine respiratory syncytial viruses. Journal of General Virology 71:3009–3014
    [Google Scholar]
  46. Walsh E. E., Hruska J. 1983; Monoclonal antibodies to respiratory syncytial virus protein: identification of the fusion protein. Journal of Virology 47:171–177
    [Google Scholar]
  47. Walsh E. E., Hall C. B., Briselli M., Brandriss M. W., Schlesinger J. L. 1987; Immunization with glycoprotein subunits of respiratory syncytial virus to protect cotton rats against viral infection. Journal of Infectious Diseases 155:1198–1204
    [Google Scholar]
  48. Wertz G. W., Collins P. L., Huang Y., Gruber C., Levine S., Ball L. A. 1985; Nucleotide sequence of the G protein of human respiratory syncytial virus reveals an unusual type of viral membrane protein. Proceedings of the National Academy of Sciences, USA 82:4075–4079
    [Google Scholar]
  49. Wertz G. W., Stott E. J., Young K. K.-Y., Anderson K., Ball L. A. 1987; Expression of the fusion protein of human respiratory syncytial virus from recombinant vaccinia virus vectors and protection of vaccinated mice. Journal of Virology 61:293–301
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-4-649
Loading
/content/journal/jgv/10.1099/0022-1317-77-4-649
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error