1887

Abstract

The capacity of several coat protein (CP) mutants of a German isolate of barley yellow mosaic bymovirus (BaYMV) to bind to nucleic acids was studied . Recombinant CP, produced by overexpression in , was purified from inclusion bodies and subsequently renatured. Binding to single-stranded (ss) RNA and ssDNA oligonucleotides was found to be cooperative and sequence non-specific. By deletion mutagenesis, several truncated CP derivatives were created and their nucleic acid-binding capacity was investigated in order to define a protein domain responsible for RNA- and DNA-binding. The nucleic acid-binding domain consists of a core which was located to an internal 23 amino acid peptide (aa 125–147) and an adjacent domain (aa 148–184) which stimulates binding.

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1996-04-01
2021-10-24
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References

  1. Baer M. L., Houser F., Loesch-Fries L. S., Gehrke L. 1994; Specific RNA binding by amino-terminal peptides of alfalfa mosaic virus CP. EMBO Journal 13:727–735
    [Google Scholar]
  2. Citovsky V., Knorr D., Schuster G., Zambryski P. 1990; The P30 movement protein of tobacco mosaic virus is a single-strand nucleic acid binding protein. Cell 60:637–647
    [Google Scholar]
  3. Citovsky V., Knorr D., Zambryski P. 1991; Gene I, a potential cell-to-cell movement protein of the cauliflower mosaic virus, encodes an RNA binding protein. Proceedings of the National Academy of Sciences, USA 88:2476–2480
    [Google Scholar]
  4. Citovsky V., Wong M. L., Shaw A. L. Prasad B. V. V., Zambryski P. 1992; Visualization and characterization of tobacco mosaic virus movement protein binding to single-stranded nucleic acids. Plant Cell 4:397–411
    [Google Scholar]
  5. Davidson A. D., Prols M., Schell J., Steinbiss H.-H. 1991; The nucleotide sequence of RNA 2 of barley yellow mosaic virus. Journal of General Virology 72:989–993
    [Google Scholar]
  6. Duggal R., Hall T. C. 1993; Identification of domains in brome mosaic virus RNA-1 and CP necessary for specific interaction and encapsidation. Journal of Virology 67:6406–6412
    [Google Scholar]
  7. Huth W., Lesemann D. 1978; Eine für die Bundesrepublik nene Virose an Wintergerste. Nachrichtenblatt des Deutschen Pflanzen-schutzdienstes 30:184–185
    [Google Scholar]
  8. Huth W., Lesemann D.-E., Paul H.-L. 1984; Barley yellow mosaic virus: purification, electron microscopy, serology, and other properties of two types of the virus. Journal of Phytopathology 111:37–54
    [Google Scholar]
  9. Kashiwazaki S., Hayano Y., Minobe Y., Omura T., Hibino H., Tsuchzaki T. 1989; Nucleotide sequence of the capsid protein gene of barley yellow mosaic virus. Journal of General Virology 70:3015–3023
    [Google Scholar]
  10. Kashiwazaki S., Minobe Y., Omura T., Hibino H. 1990; Nucleotide sequence of barley yellow mosaic virus RNA 1: a close evolutionary relationship with potyviruses. Journal of General Virology 71:2781–2790
    [Google Scholar]
  11. Matthews R. E. F. 1991 Plant Virology 3rd edn New York: Academic Press;
    [Google Scholar]
  12. Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. (editors) 1995 Virus taxonomy. Sixth report of the International Committee on Taxonomy of Viruses Vienna & New York: SpringerVerlag;
    [Google Scholar]
  13. Osman T. A. M., Hayes R. J., Buck K. W. 1992; Cooperative binding of the red clover necrotic mosaic virus movement protein to single-stranded nucleic acids. Journal of General Virology 73:223–227
    [Google Scholar]
  14. Peerenboom E., Frols M., Schell J., Steinbiss H.-H., Davidson A. D. 1992; The complete nucleotide sequence of RNA 1 of the German isolate of barley yellow mosaic virus and its comparison with a Japanese isolate. Journal of General Virology 73:1303–1308
    [Google Scholar]
  15. Schenk P. M., Steinbiss H.-H., Müller B., Schmitz K. 1993; Association of two barley yellow mosaic virus (RNA 2) encoded proteins with cytoplasmic inclusion bodies revealed by immunogold localisation. Protoplasma 173:113–122
    [Google Scholar]
  16. Schmidt J., John M., Wieneke U., Krussmann H. D., Schell J. 1986; Expression of the nodulation gene nodA in Rhizobium meliloti and localization of the gene product in the cytosol. Proceedings of the National Academy of Sciences, USA 83:9581–9585
    [Google Scholar]
  17. Schoumacher F., Erny C., Berna A., Godefroy-Colburn T., Stussi-Garaud C. 1992; Nucleic-acid-binding properties of the alfalfa mosaic virus movement protein produced in yeast. Virology 188:896–899
    [Google Scholar]
  18. Skuzeski J. M., Morris T. J. 1995; Quantitative analysis of the binding of turnip crinkle virus coat protein to RNA fails to demonstrate binding specificity but reveals a highly cooperative assembly interaction. Virology 210:82–90
    [Google Scholar]
  19. Thomas C. L., Maule A. J. 1995; Identification of the cauliflower mosaic virus movement protein RNA-binding domain. Virology 206:1145–1149
    [Google Scholar]
  20. Wei N., Morris T. J. 1991; Interactions between viral CP and a specific binding region on turnip crinkle virus RNA. Journal of Molecular Biology 222:437–443
    [Google Scholar]
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