RT Journal Article SR Electronic(1) A1 Kiguchi, Tadahiko A1 Saito, Minako A1 Tamada, TetsuoYR 1996 T1 Nucleotide sequence analysis of RNA-5 of five isolates of beet necrotic yellow vein virus and the identity of a deletion mutant JF Journal of General Virology, VO 77 IS 4 SP 575 OP 580 DO https://doi.org/10.1099/0022-1317-77-4-575 PB Microbiology Society, SN 1465-2099, AB The nucleotide sequences of RNA-5 from two laboratory isolates (D-5 and D-6) and three field isolates (SH1, S43 and R83) of beet necrotic yellow vein virus (BNYVV) were determined. Isolates D-5 and D-6, derived from a D field culture during mechanical inoculation, contained RNA-5 of molecular size 1.4 kb and 1.0 kb, respectively. The sequences of D-5, SH1, S43 and R83 were found to be at least 98% identical and from 1342 to 1347 nucleotides in length, excluding the poly(A) tail. Each contained a single open reading frame (ORF) encoding a 228 amino acid protein with a molecular mass of 26189 Da (P26). The coding sequence was bordered by a long leader of 443 to 448 nucleotides and a 3′-terminal non-coding region of 215 nucleotides. In isolate D-6, containing the smaller ∼ 1.0 kb RNA species referred to as RNA-5a, the ORF had undergone an internal deletion of 303 nucleotides. No sequence identity was found between RNA-5 and either RNA-3 or RNA-4, except for the 5′-terminal nine residues and for approximately the 3′-terminal 200 residues. Thus, the genome organization of BNYVV RNA-5 is very similar to that of RNA-3 and RNA-4, both of which are essential for survival of BNYVV in nature. Although RNA-5 is not essential, it may be associated with symptom expression of BNYVV., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-77-4-575