@article{mbs:/content/journal/jgv/10.1099/0022-1317-77-3-541, author = "Shimizu, Takumi and Toriyama, Shigemitsu and Takahashi, Mami and Akutsu, Katsumi and Yoneyama, Katsuyoshi", title = "Non-viral sequences at the 5′ termini of mRNAs derived from virus-sense and virus-complementary sequences of the ambisense RNA segments of rice stripe tenuivirus", journal= "Journal of General Virology", year = "1996", volume = "77", number = "3", pages = "541-546", doi = "https://doi.org/10.1099/0022-1317-77-3-541", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-77-3-541", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The three small segments of the four RNAs of the rice stripe tenuivirus (RSV) genome have an ambisense coding strategy. The mRNA transcripts corresponding to open reading frames for the non-structural protein (NS4) and nucleocapsid protein (N), which are encoded on virus-sense (v) RNA 4 and virus-complementary sense (vc) RNA 3, respectively, were recovered from polysomes of RSV-infected wheat leaves, and their 5′ termini were analysed. The mRNAs derived from both v and vc sequences contained from 10 to 23 non-viral bases at their 5′ termini. Results of nucleotide sequence similarity analyses indicated that these non-viral heterogenous sequences may be derived from host cellular mRNAs. Taken together, these results suggest that the viral mRNA transcription of either v or vc sequences of ambisense segments of RSV is primed by non-viral oligonucleotides in vivo.", }