1887

Abstract

A short non-coding region (SNR) commonly exists between the E5 and L2 open reading frames of human papillomaviruses (HPVs). Except for the poly(A) signal for early gene transcripts, no biological functions have been discovered for the SNR. To test a possible promoter-like activity of the SNR, we carried out CAT reporter assays using constructs containing the SNRs from HPV-16, -18 and -33 linked to a promoterless CAT gene. We reproducibly observed enhanced expression of CAT gene by the SNRs. Co-expression of a transcriptional activator (LAP/NF-IL6) or deletion of the poly(A) signal augmented the promoter-like activity of the SNRs. RNase protection assays revealed a LAP-inducible CAT mRNA properly initiated from the HPV-16 SNR. These results may suggest that the SNR has a promoter activity that is regulated by keratinocyte differentiation.

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1996-03-01
2021-10-19
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References

  1. Akira S., Kishimoto T. 1992; IL-6 and NF-IL6 in acute-phase response and viral infection. Immunological Review 127:25–50
    [Google Scholar]
  2. Akira S., Isshiki H., Sugita T., Tanabe O., Kinoshita S., Nishio Y., Nakajima T., Hirano T., Kishimoto T. 1990; A nuclear factor for IL-6 expression (NF-IL6) is a member of a C/EBP family. EMBO Journal 9:1897–1906
    [Google Scholar]
  3. Baker C. C., Howley P. M. 1987; Differential promoter utilization by the bovine papillomavirus in transformed cells and productively infected wart tissues. EMBO Journal 6:1027–1035
    [Google Scholar]
  4. Chow L. T., Nasseri M., Wolinsky S. M., Broker T. R. 1987a; Human papillomavirus types 6 and 11 mRNAs from genital condylomata acuminata. Journal of Virology 61:2581–2588
    [Google Scholar]
  5. Chow L. T., Reilly S. S., Broker T. R., Taichman L B. 1987b; Identification and mapping of human papillomavirus type 1 RNA transcripts recovered from plantar warts and infected epithelial cell cultures. Journal of Virology 61:1913–1918
    [Google Scholar]
  6. Cole S. T., Streeck R. E. 1986; Genome organization and nucleotide sequence of human papillomavirus type 33, which is associated with cervical cancer. Journal of Virology 58:991–995
    [Google Scholar]
  7. Cole S. T., Danos O. 1987; Nucleotide sequence and comparative analysis of the human papillomavirus type 18 genome. Phylogeny of papillomaviruses and repeated structure of the E6 and E7 gene products. Journal of Molecular Biology 193:599–608
    [Google Scholar]
  8. Descombes P., Schibler U. 1991; A liver-enriched transcriptional activator protein, LAP, and a trranscriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569–579
    [Google Scholar]
  9. Descombes P., Chojkier M., Lichtsteiner S., Falvey E., Schibler U. 1990; LAP, a novel member of the C/EBP gene family, encodes a liver-enriched transcriptional activator protein. Genes and Development 4:1541–1551
    [Google Scholar]
  10. Fujikawa K., Furuse M., Uwabe K.-I., Maki H., Yoshie O. 1994; Nuclear localization and transforming activity of human papillomavirus type 16 E7-β-kgalactosidase fusion protein: characterization of the nuclear localization sequence. Virology 204:789–793
    [Google Scholar]
  11. Galloway D. A., McDougall J. K. 1989; Human papillomaviruses and carcinomas. Advances in Virus Research 37:125–171
    [Google Scholar]
  12. Ghosh D. 1990; A relational database of transcription factors. Nucleic Acids Research 18:1749–1756
    [Google Scholar]
  13. Gorman C. M., Moffat L. F., Howard B. H. 1982; Recombinant genomes which express chloramphenicol acetyl transferase in mammalian cells. Molecular and Cellular Biology 2:1044–1051
    [Google Scholar]
  14. Imagawa M., Osada S., Koyama Y., Suzuki T., Hirom P. C., Diccianni M. B., Morimura S., Muramatsu M. 1991; SF-B that binds to a negative element in glutathione transferase P gene is similar or identical to trans-activator LAP/1L6-DBP. Biochemical and Biophysical Research Communications 19:293–300
    [Google Scholar]
  15. Kyo S., Inoue M., Nishio Y., Nakanishi K., Akira S., Inoue H., Yutsudo M., Tanizawa O., Hakura A. 1993; NF-IL6 represses early gene expression of human papillomavirus type 16 through binding to the noncoding region. Journal of Virology Y67:1058–1066
    [Google Scholar]
  16. McCaffery R. E., Jackson M. E. 1994; An element binding a C/EBP-related transcription factor contributes to negative regulation of the bovine papillomavirus type 4 long control region. Journal of General Virology 75:3047–3056
    [Google Scholar]
  17. McKnight S. L., Kingsbury R. 1982; Transcriptional control signals of a eukaryotic protein-coding gene. Science 217:316–324
    [Google Scholar]
  18. Maki H., Saito S., Ibaraki T., Ichijo M., Yoshie O. 1991; Use of universal and type-specific primers in the polymerase chain reaction for the detection and typing of genital human papillomaviruses. Japanese Journal of Cancer Research 82:411–419
    [Google Scholar]
  19. Ptister H. 1987; Human papillomaviruses and genital cancer. Advances in Cancer Research 48:113–147
    [Google Scholar]
  20. Proudfoot N. 1991; Poly(A) signals. Cell 64:671–674
    [Google Scholar]
  21. Rohlfs M., Winkenbach S., Meyer S., Rupp T., Durst M. 1991; Viral transcription in human eratinocyte cell lines immortalized by human papillomavirus type-16. Virology 183:331–342
    [Google Scholar]
  22. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Seedorf K., Krammer G., Durst M., Suhai S., Rowekamp W. G. 1985; Human papillomavirus type 16 DNA sequence. Virology 145:181–185
    [Google Scholar]
  24. Zinn K., Dimaio D., Maniatis T. 1983; Identification of two distinct regulatory regions adjacent to the human β- interferon gene. Cell 34:865–879
    [Google Scholar]
  25. Zur Hausen H. 1989; Papillomaviruses as carcinomaviruses. Advances in Viral Oncology 8:1–26
    [Google Scholar]
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