1887

Abstract

Sequencing of the S1 genes of nine Australian strains of infectious bronchitis virus (IBV) identified two genotypically distinct groups of strains. The strains Vic S, V5/90, N1/62, N3/62, N9/74 and N2/75 comprised group I, sharing 80.7–98.3% identity in their deduced amino acid sequences. All group I strains were able to replicate in the trachea and kidney but only four strains, Vic S, N1/62, N9/74 and N2/75, were nephropathogenic, the latter three causing mortalities ranging from 32 to 96%. Group II contained strains N1/88, Q3/88 and V18/91 which only replicated in the trachea, inducing no mortalities. These viruses showed 72.3–92.8% amino acid identity to each other and only 53.8–61.7% identity to viruses of the first group. They were also distinct from the Massachusetts 41 and D1466 strains (47.5–55.7% amino acid identity). Thus N1/88, Q3/88 and V18/91 form a new group of viruses which are genotypically distinct from all previously characterized IBV strains. No definite correlations were established between the S1 amino acid sequences and the nephropathogenicity of strains.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-3-413
1996-03-01
2022-05-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/3/JV0770030413.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-3-413&mimeType=html&fmt=ahah

References

  1. Boursnell M. E. G., Brown T. D. K., Foulds I. J., Green P. F., Tomley F. M., Binns M. M. 1987; Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. Journal of General Virology 68:57–77
    [Google Scholar]
  2. Cavanagh D., Davis P. J., Darbyshire J. H., Peters R. W. 1986; Coronavirus IBV:virus retaining spike glycopolypeptide S2 but not SI is unable to induce virus-neutralizing or haemagglutination-inhibiting antibody, or induce chicken tracheal protection. Journal of General Virology 67:1435–1442
    [Google Scholar]
  3. Cavanagh D., Davis P. J., Mockett A. P. A. 1988; Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes. Virus Research 11:141–150
    [Google Scholar]
  4. Cavanagh D., Davis P. J., Cook J. K. A., Li D., Kant A., Koch G. 1992; Location of the amino acid differences in the SI spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus. Avian Pathology 21:33–43
    [Google Scholar]
  5. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  6. Higgins D. G., Sharp P. M. 1988; CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237–244
    [Google Scholar]
  7. Ignjatovic J., McWaters P. G. 1991; Monoclonal antibodies to three structural proteins of avian infectious bronchitis virus: characterization of epitopes and antigenic differentiation of Australian strains. Journal of General Virology 72:2915–2922
    [Google Scholar]
  8. Ignjatovic J., Galli L. 1994; The SI glycoprotein but not N or M proteins of avian infectious bronchitis virus induces protection in vaccinated chickens. Archives of Virology 138:117–134
    [Google Scholar]
  9. Ignjatovic J., Galli L. 1995; Immune responses to structural proteins of avian infectious bronchitis virus. Avian Pathology 24:313–332
    [Google Scholar]
  10. Kusters J. G., Niesters H. G. M., Lenstra J. A., Horzinek M. C., van der Zeijst B. A. M. 1989a; Phylogeny of antigenic variants of avian coronavirus IBV. Virology 169:217–221
    [Google Scholar]
  11. Kusters J. G., Jager E. J., Lenstra J. A., Koch G., Posthumus W. P. A., Meloen R. H., van der Zeijst B. A. M. 1989b; Analysis of an immunodominant region of infectious bronchitis virus. Journal of Immunology 143:2692–2698
    [Google Scholar]
  12. Kusters J. G., Jager E. J., Niesters H. G. M., van der Zeijst B. A. M. 1990; Sequence evidence for RNA recombination in field isolates of avian coronavirus infectious bronchitis virus. Vaccine 8:605–608
    [Google Scholar]
  13. Kwon H. M., Jackwood M. W. 1995; Molecular cloning and sequence comparison of the S1 glycoprotein of the Gray and JMK strains of avian infectious bronchitis virus. Virus Genes 9:219–229
    [Google Scholar]
  14. Niesters H. G. M., Lenstra J. A., Spaan W. J. M., Zuderveld A. J., Bleumink-Pluym N. M. C., Hong F., van Scharrenburg G. J. M., Horzinek M. C., van der Zeijst B. A. M. 1986; The peplomer protein sequence of the M41 strain of coronavirus IBV and its comparison with Beaudette strains. Virus Research 5:253–263
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406–425
    [Google Scholar]
  16. Siller W. G., Cumming R. B. 1974; The histopathology of an interstitial nephritis in the fowl produced experimentally with infectious bronchitis virus. Journal of Pathology 114:163–173
    [Google Scholar]
  17. Taguchi F., Kubo H., Takahashi H., Suzuki H. 1995; Localization of neuro virulence determinant for rats on the SI subunit of murine coronavirus JHMV. Virology 208:67–74
    [Google Scholar]
  18. Wadey C. N., Faragher J. T. 1981; Australian infectious bronchitis viruses: identification of nine subtypes by a neutralization test. Research in Veterinary Science 30:70–74
    [Google Scholar]
  19. Wang L., Junker D., Collisson E. W. 1993; Evidence of natural recombination within the SI gene of infectious bronchitis virus. Virology 192:710–716
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-3-413
Loading
/content/journal/jgv/10.1099/0022-1317-77-3-413
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error