1887

Abstract

The full-length spike (S) gene of porcine respiratory coronavirus (PRCV) was inserted into the genome of human adenovirus type 5 downstream of the early transcription region 3 promoter. The recombinant virus replicated in cultures of the swine testicle ST cell line and directed the synthesis of S antigen with a maximum yield of approximately 26 µg per 10 cells. The antigen was cell-associated except in the late phase of the infection, when a small amount (3.5 µg per 10 cells) was released. The cell-associated antigen consisted of polypeptides of molecular mass 160 kDa and 175 kDa, comigrating with the authentic precursor S′ and the mature S protein of PRCV, respectively. The extracellular recombinant antigen corresponded to the 175 kDa mature protein. Some recombinant S protein was exposed on the cell surface and was recognized by neutralization-mediating anti-S monoclonal antibodies. Piglets, inoculated oronasally with the recombinant adenovirus vector developed PRCV-neutralizing serum antibodies and were partially protected against PRCV challenge, demonstrating the potential of live adenovirus as vaccine vector.

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1996-02-01
2024-03-19
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References

  1. Andrew M. E., Boyle D. B., Whitefeld P. L., Lockett L. J., Anthony I. D., Bellamy A. R., Both G. W. 1990; The immunogenicity of VP7, a rotavirus antigen resident in the endoplasmic reticulum is enhanced by cell-surface expression. Journal of Virology 64:4776–4783
    [Google Scholar]
  2. Callebaut P., Debouck P., Pensaert M. 1982; Enzyme-linked immunosorbent assay for the detection of the coronavirus–like agent and its antibodies in pigs with porcine epidemic diarrhea. Veterinary Microbiology 7:295–306
    [Google Scholar]
  3. Callebaut P., Correa I., Pensaert M., Jiménez G., Enjuanes L. 1988; Antigenic differentiation between transmissible gastroenteritis virus of swine and a related porcine respiratory coronavirus. Journal of General Virology 69:1725–1730
    [Google Scholar]
  4. Callebaut P., Pensaert M., Enjuanes L. 1994; Construction of a recombinant adenovirus for the expression of the glycoprotein S antigen of porcine respiratory coronavirus. Advances in Experimental Medicine and Biology 342:469–470
    [Google Scholar]
  5. Charlton K. M., Artois M., Prevec L., Campbell J. B., Casay G. A., Wandeler A. I., Armstrong J. 1992; Oral rabies vaccination of skunks and foxes with a recombinant human adenovirus vaccine. Archives of Virology 123:169–179
    [Google Scholar]
  6. Correa I., Jiménez G., Sune C., Bullido M. J., Enjuanes L. 1988; Antigenic structure of the E2 glycoprotein from transmissible gastroenteritis coronavirus. Virus Research 10:77–94
    [Google Scholar]
  7. Delmas B., Gelfi J., Laude H. 1986; Antigenic structure of transmissible gastroenteritis virus. II. Domains in the peplomer glycoprotein. Journal of General Virology 67:1405–1418
    [Google Scholar]
  8. Godet M., Raschaert D., Laude H. 1991; Processing and antigenicity of entire and anchor-free spike glycoprotein S of coronavirus TGEV expressed by recombinant baculovirus. Virology 185:732–740
    [Google Scholar]
  9. Graham F. L., Prevec L. 1991; Manipulation of adenovirus vectors. Methods in Molecular Biology 7:109–128
    [Google Scholar]
  10. Pensaert M., Callebaut P., Vergote J. 1986; Isolation of a porcine respiratory, non–enteric coronavirus related to transmissible gastroenteritis. Veterinary Quarterly 8:257–261
    [Google Scholar]
  11. Pulford D. J., Britton P. 1991; Intracellular processing of the porcine coronavirus transmissible gastroenteritis virus spike protein expressed by recombinant vaccinia virus. Virology 182:765–773
    [Google Scholar]
  12. Rasschaert D., Duarte M., Laude H. 1990; Porcine respiratory coronavirus differs from transmissible gastroenteritis virus by a few genomic deletions. Journal of General Virology 71:2599–2607
    [Google Scholar]
  13. Sanchez C. M., Jimenez G., Laviada M. D., Correa L., Suné C., Bullido M. J., Gebauer F., Smerdou C., Callebaut P., Escribano J. M., Enjuanes L. 1990; Antigenic homology among coronaviruses related to transmissible gastroenteritis virus. Virology 174:410–417
    [Google Scholar]
  14. Sanchez C. M., Gebauer F., Suné C., Mendez A., Dopazo J., Enjuanes L. 1992; Genetic evolution and tropism of transmissible gastroenteritis coronaviruses. Virology 190:92–105
    [Google Scholar]
  15. To L. T., Bernard S., Bottreau E. 1992; Transmissible gastroenteritis coronavirus: surface antigens induced by virulent and attenuated strains. Research in Virology 143:241–248
    [Google Scholar]
  16. Tuboly T., Nagy E., Derbyshire J. B. 1993; Potential viral vectors for the stimulation of mucosal antibody responses against enteric antigens in pigs. Research in Veterinary Science 54:345–350
    [Google Scholar]
  17. Van Reeth K., Pensaert M. 1994; Porcine respiratory coronavirus-mediated interference against influenza virus replication in the respiratory tract of feeder pigs. American Journal of Veterinary Research 55:1275–1281
    [Google Scholar]
  18. Wesseling J. G., Godeke G., Schijns V. E. C. J., Prevec L., Graham F. L., Horzinek M. C., Rottier P. J. M. 1993; Mouse hepatitis virus spike and nucleocapsid proteins expressed by adenovirus vectors protect mice against a lethal infection. Journal of General Virology 74:2061–2069
    [Google Scholar]
  19. Wold W. S. M., Gooding L. R. 1991; Region E3 of adenovirus: a cassette of genes involved in host immunosurveillance and virus–cell interactions. Virology 184:1–8
    [Google Scholar]
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