1887

Abstract

The bovine papillomavirus type 1 (BPV-1) long control region (LCR) contains at least three consensus binding sites for the transcription factor Sp1 at nucleotides (nt) 7800, 7833 and 7854. A high basal-level P expression vector consisting of an origin-deleted LCR fused to the chloramphenicol acetyltransferase (CAT) gene was utilized to determine the role of these Sp1 sites in the regulation of transcription from the BPV-1 P promoter. The three Sp1 sites were capable of binding Sp1 . Mutation of these sites in the background of the origin-deleted LCR-CAT or a wild-type LCR-CAT construct resulted in decreased basal expression from P. In addition, mutation of the Sp1 sites in the wild-type background caused a reduction in E2-transactivation potential. These data illustrate the importance of these Sp1 sites in regulating both basal and E2-transactivated P expression.

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1996-02-01
2024-04-16
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References

  1. Ahola H., Stenlund A., Moreno-Lopez J., Pettersson U. 1987; Promoters and processing sites within the transforming region of bovine papillomavirus type 1. Journal of Virology 61:2240–2244
    [Google Scholar]
  2. Androphy E. J., Lowy D. R., Schiller J. T. 1987; A peptide encoded by the bovine papillomavirus E2 trans-acting gene binds to specific sites in papillomavirus DNA. Nature 325:70–73
    [Google Scholar]
  3. Baker C. C. 1990; Bovine papillomavirus transcription. In Papillomaviruses and Human Cancer pp 91–112 Edited by Pfister H. Boca Raton, Fla.: CRC Press;
    [Google Scholar]
  4. Blitz I. L., Laimins L. 1991; The 68-kilodalton E1 protein of bovine papillomavirus is a DNA binding phosphoprotein which associates with the E2 transcriptional activator in vitro. Journal of Virology 65:649–656
    [Google Scholar]
  5. Chan W. K., Chong T., Bernard U., Klock G. 1990; Transcription of the transforming genes of the oncogenic human papillomavirus-16 is stimulated by tumor promoters through API binding sites. Nucleic Acids Research 18:763–769
    [Google Scholar]
  6. Chong T., Chan W. K., Bernard H. U. 1990; Transcriptional activation of human papillomavirus 16 by nuclear factor 1, API, steroid receptors and a possibly novel transcription factor, PVF: a model for the composition of genital papillomavirus enhancers. Nucleic Acids Research 18:465–470
    [Google Scholar]
  7. Dignam J. D., Lebovitz R. M., Roeder R. G. 1983; Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Research 11:1475–1489
    [Google Scholar]
  8. Dostatni N., Thierry F., Yaniv M. 1988; A dimer of BPV-1 E2 protein containing a protease resistant core interacts with its DNA target. EMBO Journal 7:3807–3816
    [Google Scholar]
  9. Dostatni N., Lambert P. F., Sousa R., Ham J., Howley P. M., Yaniv M. 1991; The functional BPV-1 E2 trans-activating protein can act as a repressor by preventing formation of the initiation complex. Genes & Development 5:1657–1671
    [Google Scholar]
  10. Gloss B., Bernard H. U. 1990; The E6/E7 promoter of human papillomavirus type 16 is activated in the absence of E2 proteins by a sequence-aberrant Spl distal element. Journal of Virology 64:5577–5584
    [Google Scholar]
  11. Gloss B., Yeo-Gloss M., Meisterernst M., Rogge L., Winnacker E. L., Bernard H. U. 1989; Clusters of nuclear factor I binding sites identify enhancers of several papillomaviruses but alone are not sufficient for enhancer function. Nucleic Acids Research 17:3519–3533
    [Google Scholar]
  12. Graham F. L., van der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–461
    [Google Scholar]
  13. Ham J., Dostatni N., Arnos F., Yaniv M. 1991; Several different upstream promoter elements can potentiate transactivation by the BPV-1 E2 protein. EMBO Journal 10:2931–2940
    [Google Scholar]
  14. Hoppe-Seyler F., Butz K. 1992; Activation of human papillomavirus type 18 E6-E7 oncogene expression by transcription factor Spl. Nucleic Acids Research 20:6701–6706
    [Google Scholar]
  15. Lambert P. F., Howley P. M. 1988; Bovine papillomavirus type 1 E1 replication-defective mutants are altered in their transcriptional regulation. Journal of Virology 62:4009–4015
    [Google Scholar]
  16. Li R., Knight J., Bream G., Stenlund A., Botchan M. 1989; Specific recognition nucleotides and their DNA context determine the affinity of E2 protein for 17 binding sites in the BPV-1 genome. Genes & Development 3:510–526
    [Google Scholar]
  17. Li R., Knight J. D., Jackson S. P., Tjian R., Botchan M. R. 1991; Direct interaction between Spl and the BPV enhancer E2 protein mediates synergistic activation of transcription. Cell 65:493–505
    [Google Scholar]
  18. McBride A. A., Byrne J. C., Howley P. M. 1989; E2 polypeptides encoded by bovine papillomavirus 1 form dimers through the carboxy-terminal DNA-binding domain: transactivation is mediated through the amino-terminal domain. Proceedings of the National Academy of Sciences, USA 86:510–514
    [Google Scholar]
  19. Mohr I. J., Clark R., Sun S., Androphy E. J., MacPherson P., Botchan M. 1990; Targeting the E1 replication protein to the papillomavirus origin of replication by complex formation with the E2 transactivator. Science 250:1694–1699
    [Google Scholar]
  20. Neary K., DiMaio D. 1989; Open reading frames E6 and E7 of bovine papillomavirus type 1 are both required for full transformation of mouse C127 cells. Journal of Virology 63:259–266
    [Google Scholar]
  21. Romanczuk H., Howley P. M. 1992; Disruption of either the E1 or E2 regulatory gene of human papillomavirus type 16 increases viral immortalization capacity. Proceedings of the National Academy of Sciences, USA 89:3159–3163
    [Google Scholar]
  22. Sandler A. B., Vande Pol S. B., Spalholz B. A. 1993; Repression of bovine papillomavirus type 1 transcription by the E1 replication protein. Journal of Virology 67:5079–5087
    [Google Scholar]
  23. Schiller J. T., Kleiner E., Androphy E. J., Lowy D. R., Pfister H. 1989; Identification of bovine papillomavirus E1 mutants with increased transforming and transcriptional activity. Journal of Virology 63:1775–1782
    [Google Scholar]
  24. Schiller J. T., Vass W. C., Lowy D. R. 1984; Identification of a second transforming region in bovine papillomavirus DNA. Proceedings of the National Academy of Sciences, USA 81:7880–7884
    [Google Scholar]
  25. Seo Y. S., Muller F., Lusky M., Gibbs E., Kim H. Y., Phillips B., Hurwitz J. 1993; Bovine papillomavirus (BPV)-encoded E2 protein enhances binding of E1 protein to the BPV replication origin. Proceedings of the National Academy of Sciences, USA 90:2865–2869
    [Google Scholar]
  26. Spalholz B. A., Yang Y. C., Howley P. M. 1985; Transactivation of a bovine papillomavirus transcriptional regulatory element by the E2 gene product. Cell 42:183–191
    [Google Scholar]
  27. Spalholz B. A., Lambert P. F., Yee C. L., Howley P. M. 1987; Bovine papillomavirus transcriptional regulation: localization of the E2-responsive elements of the long control region. Journal of Virology 61:2128–2137
    [Google Scholar]
  28. Spalholz B. A., Vande Pol S. B., Howley P. M. 1991; Characterization of the cis elements involved in basal and E2-transactivated expression of the bovine papillomavirus P2443 promoter. Journal of Virology 65:743–753
    [Google Scholar]
  29. Spalholz B. A., McBride A. A., Sarafi T., Quintero J. 1993; Binding of bovine papillomavirus E1 to the origin is not sufficient for DNA replication. Virology 193:201–212
    [Google Scholar]
  30. Stenlund A., Zabielski J., Ahola H., Moreno-Lopez J., Pettersson U. 1985; Messenger RNAs from the transforming region of bovine papillomavirus type 1. Journal of Molecular Biology 182:541–554
    [Google Scholar]
  31. Szymanski P., Stenlund A. 1991; Regulation of early gene expression from the bovine papillomavirus genome in transiently transfected C127 cells. Journal of Virology 65:5710–5720
    [Google Scholar]
  32. Tomic M., Sunjevaric I., Savtchenko E. S., Blumenberg M. 1990; A rapid and simple method for introducing specific mutations into any position of DNA leaving all other positions unaltered. Nucleic Acids Research 18:1656
    [Google Scholar]
  33. Ustav M., Ustav E., Szymanski P., Stenlund A. 1991; Identification of the origin of replication of bovine papillomavirus and characterization of the viral origin recognition factor E1. EMBO Journal 10:4321–4329
    [Google Scholar]
  34. Ustav E., Ustav M., Szymanski P., Stenlund A. 1993; The bovine papillomavirus origin of replication requires a binding site for the E2 transcriptional activator. Proceedings of the National Academy of Sciences, USA 90:898–902
    [Google Scholar]
  35. Vande Pol S. B., Howley P. M. 1990; A bovine papillomavirus constitutive enhancer is negatively regulated by the E2 repressor through competitive binding for a cellular factor. Journal of Virology 64:5420–5429
    [Google Scholar]
  36. Vande Pol S. B., Howley P. M. 1992; The bovine papillomavirus constitutive enhancer is essential for viral transformation, DNA replication, and the maintenance of latency. Journal of Virology 66:2346–2358
    [Google Scholar]
  37. Wilson V., Ludes-Meyers J. 1991; A bovine papillomavirus E1-related protein binds specifically to bovine papillomavirus DNA. Journal of Virology 65:5314–5322
    [Google Scholar]
  38. Winokur P., McBride A. A. 1992; Separation of the transcriptional activation and replication functions of the bovine papillomavirus-1 E2 protein. EMBO Journal 11:4111–4118
    [Google Scholar]
  39. Yang Y. C., Okayama H., Howley P. M. 1985; Bovine papillomavirus contains multiple transforming genes. Proceedings of the National Academy of Sciences, USA 821030–1034
    [Google Scholar]
  40. Yang L., Li R., Mohr I. J., Clark R., Botchan M. 1991; Activation of BPV-1 replication in vitro by the transcription factor E2. Nature 353:628–632
    [Google Scholar]
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