1887

Abstract

Virus-like-particles (VLPs) of various papillomavirus (PV) types have been produced by expressing recombinant L1 proteins in eukaryotic cells. Although VLPs have the same ultrastructural appearance as native virions and their immunogenicity appears to be similar, their antigenicity has not been carefully evaluated. For this reason, the antigenicity of intact bovine PV type 1 (BPV-1) virions was compared with that of BPV-1 recombinant L1 VLPs by ELISA using a well-characterized panel of polyclonal and monoclonal antibodies generated against intact and denatured BPV-1 particles. The structural integrity of the authentic virions and recombinant VLPs was verified by electron microscopy. The specificity of antibodies raised against intact BPV-1 virions and their reactivity with VLPs revealed that the immunodominant, type-specific, conformational epitopes of intact virions were reproduced on VLPs. However, many monoclonal antibodies that define cross-reactive, non-conformational (linear) epitopes cryptic to the authentic BPV-1 virion tested positively when reacted with intact VLPs. One monoclonal antibody, which recognizes a BPV-1 and deer PV surface conformational epitope, did not react with VLPs. Therefore, although VLPs can be used to immunize animals against infection, the external exposure of broadly cross-reactive epitopes of intact L1 VLPs suggests that the use of L1 VLPs in antigenicity studies such as serological screening should be done with caution.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-2-183
1996-02-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/2/JV0770020183.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-2-183&mimeType=html&fmt=ahah

References

  1. Baker C. C. 1987; Sequence analysis of papillomavirus genomes. In The Papovaviridae vol 2 pp 321–385 Edited by Salzman N. P., Howley P. M. New York: Plenum Press;
    [Google Scholar]
  2. Baker T. S., Newcomb W. W., Olson H. H., Cowsert L. M., Olson C., Brown J. C. 1991; Structure of bovine and human papillomaviruses. Analysis by cryoelectron microscopy and three dimensional image reconstruction. Biophysical Journal 60:1445–1460
    [Google Scholar]
  3. Breitburd F., Kirnbauer R., Hubbert N. L., Nonnenmacher B., Trin-Dinh-Desmarquet C., Orth G., Schiller J. T., Lowy D. R. 1995; Immunization with virus-like particles from cottontail rabbit papillomavirus (CRPV) can protect against experimental CRPV infection. Journal of Virology 69:3959–3963
    [Google Scholar]
  4. Christensen N.D., Kreider J.W. 1990; Antibody-mediated neutralization in vivo of infectious papillomaviruses. Journal of Virology 64:3151–3156
    [Google Scholar]
  5. Christensen N. D., Kreider J. W., Cladel N. M., Patrick S. D., Welsh P. A. 1990; Monoclonal antibody-mediated neutralization of infectious human papillomavirus type 11. Journal of Virology 64:5678–5681
    [Google Scholar]
  6. Christensen N. D., Höpel R., Diangelo S.L., Cladel N.M., Patrick S.D., Welsh P.A., Budgeon L.R., Reed C.A., Kreider J.W. 1994; Assembled baculovirus-expressed human papillomavirus type 11 L1 capsid protein virus-like particles are recognized by neutralizing monoclonal antibodies and induce high titres of neutralizing antibodies. Journal of General Virology 75:2271–2276
    [Google Scholar]
  7. Cowsert L. M., Lake P., Jenson A. B. 1987; Topographical and conformational epitopes of bovine papillomavirus type 1 defined by monoclonal antibodies. Journal of the National Cancer Institute 79:1053–1057
    [Google Scholar]
  8. Craweord L. V., Crawford E. M. 1963; A comparative study of polyoma and papilloma viruses. Virology 21:258–263
    [Google Scholar]
  9. de Villiers E.-M. 1989; Heterogeneity of the human papillomavirus group. Journal of Virology 63:4898–4903
    [Google Scholar]
  10. Finch J. I., Klug A. 1965; The structure of viruses of the papilloma-polyoma type. III. Structure of rabbit papilloma virus. Journal of Molecular Biology 13:1–12
    [Google Scholar]
  11. Ghim S., Jenson A. B. 1993; Identification of conformational epitopes of the BPV-1 capsid recognized by competitive inhibition of sera from infected or immunized animals. Pathobiology 61:138–144
    [Google Scholar]
  12. Ghim S., Christensen N. D., Kreider J. W., Jenson A. B. 1991; Comparison of neutralization of BPV1 infection of C127 cells and bovine fetal skin xenografts. International Journal of Cancer 49:285–289
    [Google Scholar]
  13. Ghim S., Schlegel R., Hines J. F., Christensen N. D., Kreider J., Jenson A. B. 1994; Comparison of PV conformational epitopes expressed by LI proteins in mammalian (cos) and insect (SO) cells. In Immunology of Human Papillomaviruses pp 147–153 Edited by Stanley M. A. New York: Plenum Press;
    [Google Scholar]
  14. Gorra J.B., Lancaster W.D., Kurman R.J., Jenson A. B. 1985; Bovine papillomavirus type 1 monoclonal antibodies. Journal of the National Cancer Institute 75:121–125
    [Google Scholar]
  15. Hagensee M.E., Yaegashi N., Galloway D.A. 1993; Selfassembly of human papillomavirus type l capsid by expression of L 1 protein alone or by coexpression of the L1 and L2 capsid proteins. Journal of Virology 67:315–322
    [Google Scholar]
  16. Hagensee M. E., Olson N. H., Baker T. S., Galloway N.A. 1994; Three-dimensional structure of vaccinia virus produced human papillomavirus type 1 capsids. Journal of Virology 68:4503–4505
    [Google Scholar]
  17. Jarrett W. F., O’Neill B. W., Gaukroger J. M., Smith K. T., Laird H. M., Campo M. S. 1990; Studies on vaccination against papillomaviruses: the immunity after infection and vaccination with bovine papillomaviruses of different types. Veterinary Record 126:473–475
    [Google Scholar]
  18. Jin X. W., Cowsert L. M., Pilacinski W. P., Jenson A. B. 1989; Identification of L2 open reading frame gene products of bovine papillomavirus type-1 by monoclonal antibodies. Journal of General Virology 10:1133–1140
    [Google Scholar]
  19. Kirnbauer R., Booy F., Cheng N., Lowy D. R., Schiller J. T. 1992; Papillomavirus L1 major capsid protein self-assembles into virus-like particles that are highly immunogenic. Proceedings of the National Academy of Sciences, USA 89:12180–12184
    [Google Scholar]
  20. Lambert P. F., Howley P. M. 1988; Bovine papillomavirus type 1 E1 replication-defective mutants are altered in their transcriptional regulation. Journal of Virology 62:4009–4015
    [Google Scholar]
  21. Lancaster W. D., Olson C. 1978; Demonstration of two distinct classes of bovine papillomavirus. Virology 89:372–379
    [Google Scholar]
  22. Lim P. S., Jenson A. B., Cowsert L., Nakai Y., Lim L. Y., Jin X. W., Sundberg J. P. 1990; Distribution and specific identification of papillomavirus major capsid protein epitopes by immunocytochemistry and epitope scanning of synthetic peptides. Journal of Infectious Diseases 162:1263–1269
    [Google Scholar]
  23. Nakai Y., Lancaster W. D., Lim L. Y., Jenson A. B. 1986; Monoclonal antibodies to genus- and type-specific papillomavirus structural antigens. Intervirology 25:30–37
    [Google Scholar]
  24. Pfeister H. 1984; Biology and biochemistry of papillomaviruses. Reviews of Physiology, Biochemistry and Pharmacology 99:111–181
    [Google Scholar]
  25. Pfeister H. 1987; Papillomaviruses: general descriptions, taxonomy and classification. In The Papovaviridae vol 2 pp 1–38 Edited by Salzman N. P., Howley P. M. New York: Plenum Press;
    [Google Scholar]
  26. Rose R. C., Reichman R. C., Bonnez W. 1994; Human papillomavirus (HPV) type 11 recombinant virus-like particles induce the formation of neutralizing antibodies and detect HPV specific antibodies in human sera. Journal of General Virology 75:2075–2079
    [Google Scholar]
  27. Yaegashi N., Jenison S. A., Valentine J. M., Dunn M., Taichman L. B., Baker D. A., Galloway D. A. 1991; Characterization of murine polyclonal antisera and monoclonal antibodies generated against intact and denatured human papillomavirus type 1 virions. Journal of Virology 65:1578–1583
    [Google Scholar]
  28. Viscidi R. P., Shah K. V. 1992; Immune response to genital infections. In Sexually Transmitted Diseases pp 239–260 Edited by Quinn T. C. New York: Raven Press;
    [Google Scholar]
  29. Zhou J., Stenzel D. J., Sun X. Y., Frazer I. H. 1993; Synthesis and assembly of infectious bovine papillomavirus particles in vitro. Virology 74:763–768
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-2-183
Loading
/content/journal/jgv/10.1099/0022-1317-77-2-183
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error