1887

Abstract

The hepatitis B virus X-protein (HBx) has been expressed in both as an unfused protein and with an N-terminal hexaHis-containing fusion sequence. Both forms of HBx, after purification, displayed a potent AMP kinase activity, in which HBx phosphorylates AMP to ADP, using ATP as the exclusive phosphate donor. We also found that HBx has previously unreported GTPase and GTP-ADP nucleoside diphosphate kinase activities.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-2-173
1996-02-01
2023-02-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/2/JV0770020173.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-2-173&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. Journal of Molecular Biology 215:403–410
    [Google Scholar]
  2. Benn J., Schneider R. J. 1994; Hepatitis B virus HBx protein activates Ras-GTP complex formation and establishes a Ras, Raf, MAP kinase signaling cascade. Proceedings of the National Academy of Sciences, USA 91:10350–10354
    [Google Scholar]
  3. Chestnut J. D., Stephens J. H., Damus M. E. 1992; The interaction of RNA polymerase II with the adenovirus-2 major late promotor is precluded by phosphorylation of the C-terminal domain of subunit Ila. Journal of Biological Chemistry 267:10500–10506
    [Google Scholar]
  4. De-Medina T., Shaul Y. 1994; Functional and structural similarity between the X protein of hepatitis B virus and nucleoside diphosphate kinases. FEBS 351:423–426
    [Google Scholar]
  5. De-Medina T., Haviv I., Noiman S., Shaul Y. 1994; The X-protein of hepatitis B virus has a ribo/deoxy ATPase activity. Virology 202:401–407
    [Google Scholar]
  6. Diedrichs K., Schulz G. E. 1991; The refined structure of the complex between adenyl kinase from beef heart mitochondrial matrix and its substrate AMP. Journal of Molecular Biology 217:541–549
    [Google Scholar]
  7. Feitelson M. A., Zhu M., Duan L. X., London W. T. 1993; Hepatitis B x antigen and p53 are associated in vitro and in liver tissues from patients with primary hepatocellular carcinoma. Oncogene 8:1109–1117
    [Google Scholar]
  8. Kekule A. S., Lauer U., Luber B., Hofschneider P. H. 1993; Hepatitis B virus transactivator HBx uses a tumour promotor signalling pathway. Nature 361:742–745
    [Google Scholar]
  9. Kim C. M., Koike K., Saito L., Miyamura T., Jay G. 1991; HBx gene of hepatitis B virus induces liver cancer in transgenic mice. Nature 351:317–320
    [Google Scholar]
  10. Murakami S., Cheong J., Ohno S., Matsushima K., Kaneko S. 1994; Transactivation of human hepatitis B virus X protein, HBx, operates through a mechanism distinct from protein kinase C and okadaic acid activation pathways. Virology 199:243–246
    [Google Scholar]
  11. Pearson M. T., Lipmann D. J. 1988; Improved tools for biological sequence comparison. Proceedings of the National Academy of Sciences, USA 85:2444–2448
    [Google Scholar]
  12. Qadri I., Maguire H. F., Siddiqui A. 1995; Hepatitis B virus transactivator protein X interacts with the TATA-binding protein. Proceedings of the National Academy of Sciences, USA 92:1003–1007
    [Google Scholar]
  13. Rossner M. T. 1992; Hepatitis B virus X-gene product: a promiscuous transcriptional activator (review). Journal of Medical Virology 36:101–117
    [Google Scholar]
  14. Studier F. W., Rosenberg A. H., Dunn J. J., Dubendorff J. W. 1990; Use of T7 RNA polymerase to direct expression of cloned genes. Methods in Enzymology 185:60–89
    [Google Scholar]
  15. Szmunness W. 1978; Hepatocellular carcinoma and the hepatitis B virus: evidence for a causal association. Progress in Medical Virology 24:40–69
    [Google Scholar]
  16. Wang W., Carey M., Gralla J. D. 1992; Polymerase II promotor activation: closed complex formation and ATP-driven start site opening. Science 255:450–453
    [Google Scholar]
  17. Wang X. W., Forrester K., Yeh H., Feitelson M. A., Gu J.-R., Harris C. C. 1994; Hepatitis B virus X protein inhibits p53 sequence-specific DNA binding, transcriptional activity and association with transcription factor ERCC3. Proceedings of the National Academy of Sciences, USA 91:2230–2234
    [Google Scholar]
  18. Wu J. Y., Zhou Z. Y., Cartwright C. A., Robinson W. S. 1990; The hepatitis B virus encoded transcriptional transactivator HBx appears to be a novel protein serine-threonine kinase. Cell 63:687–695
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-2-173
Loading
/content/journal/jgv/10.1099/0022-1317-77-2-173
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error