1887

Abstract

Partial survival of lyophilized scrapie agent has been reported previously following exposure to dry heat at 360 °C for 1 h, and led to speculation that scrapie-like agents might not be completely inactivated by incineration. However, it is known that dried infectivity is more difficult to inactivate by heat than that in hydrated samples. In this present study it was shown that the infectivity in macerates of mouse-brain infected with the ME7 strain of scrapie agent was not completely inactivated by exposure to dry heat at temperatures up to 180 °C for 1 h but the titre of surviving infectivity reduced progressively as the temperature was increased. No infectivity was recovered after a 1 h exposure at 200 °C. These data suggest that scrapie-like agents are unlikely to survive incineration.

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1996-12-01
2021-10-28
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References

  1. Asher D. C., Pomeroy K. L., Murphy L., Rohwer R. G., Gibbs C. J., Gajdusek D. C. 1986; Practical inactivation of scrapie agent on surfaces. Abstracts of the IXth International Congress of Infectious and Parasitic Diseases Munich 1986
    [Google Scholar]
  2. Brown P., Rohwer R. G., Gajdusek D. C. 1986; Newer data on the inactivation of scrapie virus or Creutzfeldt–jakob disease virus in brain tissue. Journal of Infectious Diseases 153:1145–1148
    [Google Scholar]
  3. Brown P., Liberski P. P., Wolff A., Gajdusek D. C. 1990; Resistance of scrapie infectivity to steam autoclaving after formaldehyde fixation and limited survival after ashing at 360 °C: practical and theoretical implications. Journal of Infectious Diseases 161:467–472
    [Google Scholar]
  4. Department of the Environment 1995; Environmental Protection Act 1990 Part 1: Secretary of State’s Guidance -clinical remains incinerator processes under 1 ton an hour. PG5/3 (1995). August 1995. DD 301228 8/95 CB G 3396 10170. London: HMSO;
    [Google Scholar]
  5. Dickinson A. G., Fraser H. 1969; Modification of the pathogenesis of scrapie by treatment of the agent. Nature 222:892–893
    [Google Scholar]
  6. Dickinson A. G., Meikle V. M. H., Fraser H. 1968; Identification of a gene which controls the incubation period of some strains of scrapie in mice. Journal of Comparative Pathology 78:293–299
    [Google Scholar]
  7. Ernst D. R., Race R. E. 1993; Comparative analysis of scrapie inactivation. Journal of Virological Methods 41:193–202
    [Google Scholar]
  8. Fraser H., Dickinson A. G. 1968; The sequential development of the brain lesions of scrapie in three strains of mice. Journal of Comparative Pathology 78:301–311
    [Google Scholar]
  9. Karber G. 1931; Beitrag zur kollectiven Behandlung pharmako-logische Reihen versuche. Archives of Experimental Pathology and Pharmacology 162:480–483
    [Google Scholar]
  10. Kimberlin R. H., Walker C. A., Millson G. C., Taylor D. M., Robertson P., Tomlinson A. H., Dickinson A. G. 1983; Disinfection studies with two strains of mouse-passaged scrapie agent. Journal of the Neurological Sciences 59:355–369
    [Google Scholar]
  11. Microbiology Advisory Committee 1993; Sterilization, disinfection and cleaning of medical equipment. Guidance on Decontamination to the Department of Health Medical Devices Directorate London: Medical Devices Directorate Publication ISBN 1;85839–1199
    [Google Scholar]
  12. Taylor D. M., Femie K. 1996; Exposure to autoclaving or sodium hydroxide extends the dose-response curve of the 263K strain of scrapie agent in hamsters. Journal of General Virology 77:811–813
    [Google Scholar]
  13. Taylor D. M., Fraser H., McConnell I., Brown D. A., Brown K. L., Lamza K. A., Smith G. R. A. 1994; Decontamination studies with the agents of bovine spongiform encephalopathy and scrapie. Archives of Virology 139:313–326
    [Google Scholar]
  14. Taylor D. M., Woodgate S. L., Atkinson M. J. 1995; Inactivation of the bovine spongiform encephalopathy agent by rendering procedures. Veterinary Record 137:605–610
    [Google Scholar]
  15. Taylor D. M., Woodgate S. L., Fleetwood A. J. 1996; Scrapie agent survives rendering procedures. Abstracts of the Jubilee Meeting of the Association of Veterinary Teachers and Research Workers. pp. 33 Scarborough:1–4 April
    [Google Scholar]
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