1887

Abstract

Molluscum contagiosum virus (MCV) and vaccinia virus (VV) are serologically unrelated poxviruses with a disparate genome composition (MCV, 66% G + C; VV, 33% G + C). Molecular studies of MCV have been hindered by the inability to propagate the virus in cells cultured . We sequenced 7765 bp of MCV DNA cloned from four widely spaced regions throughout the MCV genome and identified a total of 11 potential open reading frames (ORF), designated CX1-11. These include MCV homologues of the VV genes encoding protein kinase 2, structural protein VP8, RNA polymerase 35 kDa subunit and 3β-hydroxysteroid dehydrogenase. The position and orientation of the MCV ORFs was collinear to the VV genome, with the exception of the region around ORF CX11 which is inverted in the MCV genome.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-12-3113
1996-12-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/12/JV0770123113.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-12-3113&mimeType=html&fmt=ahah

References

  1. Amegadzie B. Y., Ahn B.-Y., Moss B. 1991; Identification, sequence, and expression of the gene encoding a Mr 35, 000 subunit of the vaccinia virus DNA-dependent RNA polymerase. Journal of Biological Chemistry 76:13712–13718
    [Google Scholar]
  2. Blake N. W., Porter C. D., Archard L. C. 1991; Characterization of a Molluscum contagiosum virus homolog of the vaccinia virus p37K major envelope antigen. Journal of Virology 65:3583–3589
    [Google Scholar]
  3. Binns M. M., Boursnell M. E. G., Skinner M. A. 1992; Gene translocation in poxviruses: the fowlpox virus thymidine kinase gene is flanked by 15 bp direct repeats and occupies the locus which in vaccinia virus is occupied by the ribonucleotide reductase large subunit gene. Virus Research 24:161–172
    [Google Scholar]
  4. Bugert J., Rosen-Wolff A., Darai G. 1989; Genomic characterization of Molluscum contagiosum virus type I: identification of the repetitive DNA sequences in the viral genome. Virus Genes 3:159–173
    [Google Scholar]
  5. Bugert J. J., Raab K., Rosen-Wolff A., Janssen W., Darai G. 1993; Determination of the position of the boundaries of the terminal repetitive sequences within the genome of Molluscum contagiosum virus type 1 by DNA nucleotide sequence analysis. Virology 192:391–396
    [Google Scholar]
  6. Buller R. M. L., Burnett J., Chen W., Kreider J. 1995; Replication of Molluscum contagiosum virus. Virology 213:655–659
    [Google Scholar]
  7. Cotton D. W. K., Cooper C., Barrett D. F., Leppard B. J. L. 1987; Severe atypical Molluscum contagiosum infection in an immunocompromised host. British Journal of Dermatology 116:871–876
    [Google Scholar]
  8. Davison A. J., Moss B. 1989a; Structure of vaccinia virus early promoters. Journal of Molecular Biology 210:749–769
    [Google Scholar]
  9. Davison A. J., Moss B. 1989b; The structure of vaccinia virus late promoters. Journal of Molecular Biology 210:771–784
    [Google Scholar]
  10. Drillien R., Spehner D., Villeval D., Lecocq J.-P. 1987; Similar genetic organization between a region of fowlpox virus DNA and the vaccinia virus HindIII J fragment despite divergent location of the thymidine kinase gene. Virology 160:203–209
    [Google Scholar]
  11. Gershon P. D., Ahn B.-Y., Garfield M., Moss B. 1991; Poly(A) polymerase and a dissociable polyadenylation stimulatory factor encoded by vaccinia virus. Cell 66:1269–1278
    [Google Scholar]
  12. Goebel S. J., Johnson G. P., Perkus M. E., Davis S. W., Winslow J. P., Paoletti E. 1990; The complete DNA sequence of vaccinia virus. Virology 179:247–266
    [Google Scholar]
  13. Hadasch R. P., Bugert J. J., Janssen W., Darai G. 1993; Characterization of the genome of Molluscum contagiosum virus type 1 between the genome coordinates 0.045 and 0.075 by DNA nucleotide sequence analysis of a. 6-kb Hind III/Mlu I DNA fragment. Intervirology 36:32–43
    [Google Scholar]
  14. Hruby D. E., Ball L. A. 1982; Mapping and identification of the vaccinia virus thymidine kinase gene. Journal of Virology 43:403–409
    [Google Scholar]
  15. Johnson G. P., Goebel S. J., Paoletti E. 1993; An update on the vaccinia virus genome. Virology 196:381–401
    [Google Scholar]
  16. Kay R., McPherson J. 1987; Hybrid pUC vectors for the addition of new restriction enzyme sites to the ends of DNA fragments. Nucleic Acid Research 15:2778
    [Google Scholar]
  17. Lin S., Broyles S. S. 1994; Vaccinia protein kinase 2: a second essential serine/threonine protein kinase encoded by vaccinia virus. Proceedings of the National Academy of Sciences, USA 91:7653–7657
    [Google Scholar]
  18. Mitchell J. C. 1953; Observations on the virus of Molluscum contagiosum. British Journal of Experimental Pathology 34:44–49
    [Google Scholar]
  19. Moore J. B., Smith G. L. 1992; Steroid hormone synthesis by a vaccinia enzyme: a new type of virus virulence factor. EMBO Journal 11:1973–1980
    [Google Scholar]
  20. Porter C. D., Archard L. C. 1987; Characterization and physical mapping of Molluscum contagiosum virus DNA and location of a sequence capable of encoding a conserved domain of epidermal growth factor. Journal of General Virology 68:673–682
    [Google Scholar]
  21. Porter C. D., Archard L. C. 1992; Characterization by restriction mapping of three subtypes of Molluscum contagiosum virus. Journal of Medical Virology 38:1–6
    [Google Scholar]
  22. Postlethwaite R. 1970; Molluscum contagiosum: a review. Archives of Environmental Health 21:432–452
    [Google Scholar]
  23. Skinner M. A., Moore J. B., Binns M. M., Smith G. L., Boursnell M. E. G. 1994; Deletion of fowlpox virus homologues of vaccinia virus genes between the 3β–hydroxysteroid dehydrogenase (A44L) and DNA ligase (A50R) genes. Journal of General Virology 75:2495–2498
    [Google Scholar]
  24. Sonntag K.–C., Clauer U., Bugert J. J., Schnitzler P., Darai G. 1995; Identification and properties of the genes encoding the poly(A) polymerase and a small (22 kDa) and the largest subunit (146 kDa) of the DNA–dependent RNA polymerase of Molluscum contagiosum virus. Virology 210:471–478
    [Google Scholar]
  25. Yang W.-P., Kao S.-Y., Bauer W. R. 1988; Biosynthesis and posttranslational cleavage of vaccinia virus structural protein VP8. Virology 167:585–590
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-12-3113
Loading
/content/journal/jgv/10.1099/0022-1317-77-12-3113
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error