1887

Abstract

Jaagsiekte retrovirus (JSRV) is an exogenous type D-related retrovirus specifically associated with a contagious lung cancer of sheep (sheep pulmonary adenomatosis; SPA). Recently, epithelial tumour cells in the lungs of SPA-affected sheep were identified as major sites of JSRV replication by immunological techniques and RT-PCR amplification of part of JSRV . JSRV was not detected outside the lungs and their draining lymph nodes. However, low levels of JSRV expression in non-respiratory tissues could have been masked by co-amplification of endogenous JSRV-related sequences, which were differentiated from JSRV by the lack of a al restriction site in the PCR product. To further investigate the pathogenesis of SPA, an exogenous virus-specific hemi-nested PCR was developed utilizing primers in the U3 region of JSRV LTR, where major differences between endogenous and exogenous sequences exist. This technique was shown to be ⩾ 10-fold more sensitive than the previous PCR/al digestion method. Using this new assay the tissue distribution of JSRV in sheep with natural and experimentally induced SPA was analysed. Proviral DNA and JSRV transcripts were found in all tumours and lung secretions of SPA-affected sheep ( = 22) and in several lymphoid tissues. The mediastinal lymph nodes draining the lungs were consistently demonstrated to be infected by JSRV (10/10). JSRV transcripts were also detected in spleen (7/9), thymus (2/4), bone marrow (4/8) and peripheral blood mononuclear cells (3/7). Proviral DNA was also detected in these tissues although in a much lower proportion of cases. JSRV was not detected in 27 samples from unaffected control animals ( = 15).

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1996-12-01
2021-10-16
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References

  1. Bai J., Zhu R. J., Stedman K., Cousens C., Carlson J., Sharp J. M., DeMartini J. C. 1996; Unique long terminal repeat U3 sequences distinguish exogenous jaagsiekte sheep retrovirus associated with ovine pulmonary carcinoma from endogenous loci in the sheep genome. Journal of Virology 70:3159–3168
    [Google Scholar]
  2. Barsky S. H., Cameron R., Ossan K. E., Tomita D., Holmes E. C. 1994; Rising incidence of bronchioalveolar lung-carcinoma and unique clinicopathological features. Cancer 73:1163–1170
    [Google Scholar]
  3. Bentvelzen P., Brinkhof J. 1977; Organ distribution of exogenous murine mammary tumour virus as determined by bioassay. European Journal of Cancer 13:241–245
    [Google Scholar]
  4. Bramblett D., Hsu C. L. L., Lozano M., Earnest K., Fabritius C., Dudley J. 1995; A redundant nuclear binding protein binding site contributes to negative regulation of mouse mammary tumor virus long terminal repeat. Journal of Virology 69:7868–7876
    [Google Scholar]
  5. Bryant M. L., Marx P. A., Shiigi S. M., Wilson B. J., McNulty W. P., Gardner M. B. 1986; Distribution of type D retrovirus sequences in tissues of macaques with simian acquired immune deficiency and retroperitoneal fibromatosis. Virology 150:149–160
    [Google Scholar]
  6. Chomczynski P., Sacchi N. 1987; Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  7. DeMartini J. C., Rosadio R. H., Sharp J. M., Russel H. I., Lairmore M. D. 1987; Experimental coinduction of type D retrovirus-associated pulmonary carcinoma and lentivirus-associated lymphoid interstitial pneumonia in lambs. Journal of the National Cancer Institute 79:167–177
    [Google Scholar]
  8. Fan H. 1994; Retroviruses and their role in cancer. In The Retroviridae vol 3 pp 313–362 Edited by Levy J. A. New York: Plenum Press;
    [Google Scholar]
  9. Gardner M. B., Luciw P., Lerche N., Marx P. 1988; Non-human primate retrovirus isolates and AIDS. In Immunodeficiency Disorders and Retroviruses pp 171–226 Edited by Perk K. New York: Academic Press;
    [Google Scholar]
  10. Gazdar A. F., Linnoila R. I. 1988; The pathology of lung cancerchanging concepts and newer diagnostic techniques. Seminars in Oncology 1S:215–225
    [Google Scholar]
  11. Golovkina T. V., Chervonsky A., Dudley J. P., Ross S. R. 1992; Transgenic mouse mammary tumour virus superantigen expression prevents viral infection. Cell 69:637–645
    [Google Scholar]
  12. Gross L. 1983; Mouse mammary carcinoma. In Oncogenic Viruses pp 305–458 Edited by Gross L. New York: Pergamon Press;
    [Google Scholar]
  13. Held W., Acha-Orbea H., MacDonald H. R., Waanders G. A. 1994; Superantigens and retroviral infection: insights from mouse mammary tumour virus. Immunology Today 15:184–190
    [Google Scholar]
  14. Henrard D., Ross S. R. 1988; Endogenous mouse mammary tumor virus is expressed in several organs in addition to the lactating mammary gland. Journal of Virology 62:3046–3049
    [Google Scholar]
  15. Herring A. J., Sharp J. M., Scott F. M. M., Angus K. W. 1983; Further evidence for a retrovirus as the aetiological agent of sheep pulmonary adenomatosis (Jaagsiekte). Veterinary Microbiology 8:237–249
    [Google Scholar]
  16. Hsu C. L., Fabritius L. C., Dudley J. 1988; Mouse mammary tumor virus proviruses in T cell lymphomas lack a negative regulatory element in the long terminal repeat. Journal of Virology 62:4644–4652
    [Google Scholar]
  17. Kozma S., Osterrieth P. M., Francois C., Calberg-Bacq C. M. 1980; Distribution of mouse mammary tumour virus antigens in RIII mice as detected by immunofluorescence on tissue sections and by immunoassays in sera and organ extracts. Journal of General Virology 51:327–339
    [Google Scholar]
  18. Kwang J., Keen J., Rosati S., Tolari F. 1995; Development and application of an antibody ELISA for the marker protein of ovine pulmonary carcinoma. Veterinary Immunology and Immunopathology 47:323–331
    [Google Scholar]
  19. Lackner A. A., Rodriguez M. H., Bush C. E., Munn R. J., Kwang H. S., Moore P. F., Osborn K. G., Marx P. A., Gardner M. B., Lowenstine L. J. 1988; Distribution of a macaque immunosuppressive type D retrovirus in neural, lymphoid and salivary tissues. Journal of Virology 62:2134–2142
    [Google Scholar]
  20. Lefebre P., Berard D. S., Cordingley M. G., Hager G. L. 1991; Two regions of the mouse mammary tumor virus long terminal repeat regulate the activity of its promoter in mammary cell lines. Molecular and Cellular Biology 11:2529–2537
    [Google Scholar]
  21. LeGrand E. K., Donovan R. M., Marx P. A., Moulton J. E., Cheung A. T. W., Lewis A. E., Gardner M. B. 1985; Monocyte function in rhesus monkeys with simian acquired immune deficiency syndrome. Veterinary Immunology and Immunopathology 10:131–146
    [Google Scholar]
  22. Martin W. B., Scott F. M., Sharp J. M., Angus K. W., Norval M. 1976; Experimental production of sheep pulmonary adenomatosis. Nature 264:183–184
    [Google Scholar]
  23. Matsuzawa A., Nakano H., Yoshimoto T., Sayama K. 1995; Biology of mouse mammary tumour virus (MMTV). Cancer Letters 90:3–11
    [Google Scholar]
  24. Maul D. H., Zaiss C. P., MacKenzie M. R., Shiigi S. M., Marx P. A., Gardner M. B. 1988; Simian retrovirus D serogroup 1 has a broad cellular tropism for lymphoid and nonlymphoid cells. Journal of Virology 62:1768–1773
    [Google Scholar]
  25. Mink S., Hartig E., Jennewein P., Doppler W., Cato A. C. B. 1992; A mammary cell-specific enhancer in mouse mammary tumor virus DNA is composed of multiple regulatory elements including binding sites for CTF/NF-1 and a novel transcription factor. Molecular and Cellular Biology 12:4906–4918
    [Google Scholar]
  26. Mok E., Golovkina T. V., Ross S. R. 1992; A mouse mammary tumor virus mammary gland enhancer confers tissue-specific but not lactation-dependent expression in transgenic mice. Journal of Virology 66:7529–7532
    [Google Scholar]
  27. Palmarini M., Dewar P., De las Heras M., Inglis N. F., Dalziel R. G., Sharp J. M. 1995; Epithelial tumour cells in the lungs of sheep with pulmonary adenomatosis are major sites of replication for Jaagsiekte retrovirus. Journal of General Virology 76:2731–2737
    [Google Scholar]
  28. Palmarini M., Cousens C., Dalziel R. G., Bai J., Stedman K., DeMartini J. C., Sharp J. M. 1996; The exogenous form of Jaagsiekte retrovirus is specifically associated with a contagious lung cancer of sheep. Journal of Virology 70:1618–1623
    [Google Scholar]
  29. Perk K., Hod I. 1982; Sheep lung carcinoma: an epidemic analogue of a human neoplasm. Journal of the National Cancer Institute 69:747–750
    [Google Scholar]
  30. Perk K., Michalides R., Spiegelman S., Sholn J. 1974; Biochemical and morphological evidence for the presence of an RNA tumour virus in pulmonary carcinoma of sheep Qaagsiekte). Journal of the National Cancer Institute 46:525–537
    [Google Scholar]
  31. Rosadio R., Sharp J. M. 1992; Leukocyte frequency alterations in sheep with naturally and experimentally induced lung cancer. Medicina Veterinaria 9:49–51
    [Google Scholar]
  32. Rosadio R. H., Lairmore M. D., Russel H. I., DeMartini J. C. 1988; Retrovirus-associated ovine pulmonary carcinoma (sheep pulmonary adenomatosis) and lymphoid interstitial pneumonia. I. Lesion development and age susceptibility. Veterinary Pathology 25:475–478
    [Google Scholar]
  33. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  34. Sharp J. M. 1987; Sheep pulmonary adenomatosis: a contagious tumour and its causes. Cancer Survey 6:73–83
    [Google Scholar]
  35. Sharp J. M., Herring A. J. 1983; Sheep pulmonary adenomatosis: demonstration of a protein which cross-reacts with the major core proteins of Mason-Pfizer monkey virus and mouse mammary tumour virus. Journal of General Virology 64:2323–2327
    [Google Scholar]
  36. Sharp J. M., Martin W. B. 1983; Chronic respiratory virus infections. In Diseases of Sheep pp 12–17 Edited by Martin W. B. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  37. Sharp J. M., Angus K. W., Gray E. W., Scott F. M. M. 1983; Rapid transmission of sheep pulmonary adenomatosis (Jaagsiekte) in young lambs. Archives of Virology 78:89–95
    [Google Scholar]
  38. Silverberg E., Boring C. C., Squires T. S. 1990; Cancer statistics, 1990. CA: a Cancer Journal for Clinicians 40:9–26
    [Google Scholar]
  39. Tokunaga K., Nakamura Y., Gakata K., Fujimori K., Ohkubo M., Gawada K., Sakiyama S. 1987; Enhanced expression of a glyceraldehyde-3-phosphate dehydrogenase gene in human lung cancers. Cancer Research 47:5616–5619
    [Google Scholar]
  40. Tsubura A., Inaba M., Imai S., Murakami M., Oyaizu N., Yasumizu R., Ohnishi Y., Tanaka H., Morii S., Ikehara S. 1988; Intervention of T-cells in transportation of mouse mammary tumour virus (milk factor) to mammary cells in vivo . Cancer Research 48:6555–6559
    [Google Scholar]
  41. Varmus H. E., Quintrell N., Medeiros E., Bishop J. M., Nowinski R. C., Sarkar N. H. 1973; Transcription of mouse mammary tumor virus genes in tissues from high and low tumor incidence mouse strains. Journal of Molecular Biology 79:663–679
    [Google Scholar]
  42. Verwoerd D. W. 1990; Jaagsiekte (ovine pulmonary adenomatosis) virus. In Virus Infections of Ruminants pp 453–463 Edited by Dinter Z., Morein B. New York: Elsevier Science Publishers;
    [Google Scholar]
  43. Verwoerd D. W., Williamson A. N., de Villiers E. M. 1980; Aetiology of Jaagsiekte: transmission by means of subcellular fractions and evidence for the involvement of a retrovirus. Ondersterpoort Journal of Veterinary Research 47:275–280
    [Google Scholar]
  44. Verwoerd D. W., Tustin R. C., Payne A. L. 1985; Jaagsiekte: an infectious pulmonary adenomatosis of sheep, pp. 53–76. In Comparative Pathobiology of Viral Diseases Edited by Olsen R. G. Krakowka S., Blouksla J. R. Boca Raton: CRC Press;
    [Google Scholar]
  45. Weiss R., Teich N., Varmus H., Coffin J. 1985 RNA Tumour Viruses New York: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  46. York D. F., Vigne R., Verwoerd D. W., Querat G. 1992; Nucleotide sequence of the Jaagsiekte retrovirus, an exogenous and endogenous type D and B retrovirus of sheep and goats. Journal of Virology 66:4930–4939
    [Google Scholar]
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