%0 Journal Article %A Briggs, Carlton J. %A Tözsér, József %A Oroszlan, Stephen %T Effect of cyclosporin A on the replication cycle of human immunodeficiency virus type 1 derived from H9 and Molt-4 producer cells %D 1996 %J Journal of General Virology, %V 77 %N 12 %P 2963-2967 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-77-12-2963 %I Microbiology Society, %X The effect of cyclosporin A (CsA) on the replication of human immunodeficiency virus type 1 (HIV-1) was studied. CsA treatment inhibited virus production in chronically infected H9 and Molt-4 cells. CsA treatment of HeLaCD4-LTR/β-gal cells or extracellular viruses also inhibited infection (IC50 1 µg/ml). The intracellular CsA-binding molecule cyclophilin A was detected in HIV-1 derived from chronically infected H9 cells, but it was present at a substantially lower level in HIV-1 derived from chronically infected Molt-4 cells. The low level of cyclophilin A in viral particles derived from Molt-4 cells correlated well with their substantially lower infectivity as assayed on HeLaCD4-LTR/β-gal cells. CsA treatment of infected cells showed a dose-dependent reduction of cyclophilin A incorporation into virions; the amount of cyclophilin A incorporation was found to be dependent on the producer cell type. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-77-12-2963