1887

Abstract

The EITor typing phage D10 was characterized. The adsorption kinetics of the phage on MAK757 strain were biphasic in nature. Intracellular growth was characterized by an eclipse period, latent period and burst size which were 20 min, 25 min and 80 particles per cell respectively. The phage yield was dependent on the concentration and time of addition of DNA synthesis inhibitors such as nalidixic acid and novobiocin, and RNA synthesis inhibitors such as rifampicin. The 32 ± 0.2 kb linear double-stranded DNA molecule has unique termini. A restriction map of the phage DNA was constructed with the enzymes HI, dIII and I.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-11-2881
1996-11-01
2022-08-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/11/JV0770112881.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-11-2881&mimeType=html&fmt=ahah

References

  1. Ackermann H. W., Furniss A. L., Kasatiya S. S., Lee J. V., Mbiguino A., Newman F. S., Takeya K., Vieu J. F. 1983; Morphology of Vibrio choleras typing phages. Annales de I’Institut Pasteur/ Virologie 134:387–404
    [Google Scholar]
  2. Alonso J. C., Sarachu A. N., Grau O. 1981; DNA gyrase inhibitors block development of Bacillus subtilis bacteriophage SPOI. ]ournal of Virology 39:855–860
    [Google Scholar]
  3. Ansari M. Q., Pal S. C. 1990; Phage typing of Vibrio cholerae 01 biotype ElTor strains. Indian journal of Medical Research 91:263–265
    [Google Scholar]
  4. Basu S., Mukherjee S. 1968; Bacteriophage typing of Vibrio ElTor. Experientia 24:299–300
    [Google Scholar]
  5. Chakrabarti B. K., Chattopadhyay D. J., Ghosh A. N. 1993; Vibriophage D10 contains non-permuted DNA with cohesive ends. Journal of General Virology 74:2749–2752
    [Google Scholar]
  6. Chattopadhyay D. J., Sarker B. L., Ansari M. Q., Chakrabarti B. K., Roy M. K., Ghosh A. N., Pal S. C. 1993; New phage typing scheme for Vibrio cholerae 01 biotype ElTor strains. Journal of Clinical Microbiology 31:1579–1585
    [Google Scholar]
  7. Mukherjee S. 1978; Principles and practice of typing Vibrio cholerae . Methods in Microbiology 12:51–115
    [Google Scholar]
  8. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-11-2881
Loading
/content/journal/jgv/10.1099/0022-1317-77-11-2881
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error