1887

Abstract

The ecdysteroid UDP-glucosyltransferase () gene of multinucleocapsid nucleopolyhedrovirus (MbMNPV) has been cloned and characterized. MbMNPV potentially encodes a protein of 528 amino acids. Analysis of the substrate specificity of the MbMNPV EGT protein showed that it mirrors that of MNPV (AcMNPV) EGT. MbMNPV EGT also appears to be secreted from infected cells. Confirmation that the cloned gene encodes an active EGT was obtained by transient expression assays. Phylogenetic trees of NPVs were generated based on the alignment of baculovirus EGT sequences. These phylogenies support the classification of MbMNPV as a group II NPV that is most closely related to MNPV. Comparison of the EGT-based phylogenies with polyhedrin/granulin-based phylogenies shows that the position of AcMNPV is different in the two trees, possibly indicating that AcMNPV acquired its polyhedrin gene by recombination with another virus.

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1996-11-01
2024-11-14
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References

  1. Ayres M. D., Howard S. C., Kuzio J., Lopez-Ferber M., Possee R. D. 1994; The complete DNA sequence of Autographa Californiai nuclear polyhedrosis virus. Virology 202:586–605
    [Google Scholar]
  2. Barrett J. W., Krell P. J., Arif B. M. 1995; Characterization, sequencing and phylogeny of the ecdysteroid UDP-glucosyltransferase gene from two distinct nuclear polyhedrosis viruses isolated from Choristoneura fumiferana . Journal of General Virology 76:2447–2456
    [Google Scholar]
  3. Cameron I. R., Possee R. D. 1989; Conservation of polyhedrin gene promoter function between Autographa californica nuclear polyhedrosis virus and Mamestra brassicae nuclear polyhedrosis virus. Virus Research 12:183–200
    [Google Scholar]
  4. Coffman B. L., Tephly T. R., Irshaid Y. M., Green M. D., Smith C., Jackson M. R., Wooster R., Burchell B. 1990; Characterisation and primary sequence of a human hepatic-microsomal estriol UDP-glucosyltransferase. Archives of Biochemistry and Biophysics 281:170–175
    [Google Scholar]
  5. Croizier G., Croizier L., Quiot J. M., Leredus D. 1988; Recombination of Autographa californica and Rachiplusia ou nuclear polyhedrosis viruses in Galleria mellonella . Journal of General Virology 69:177–186
    [Google Scholar]
  6. Crook N. E., Clem R. J., Miller L. K. 1993; An apoptosis-inhibiting baculovirus gene with a zinc finger-like motif. Journal of Virology 67:2168–2174
    [Google Scholar]
  7. Doyle C. J., Hirst M. L., Cory J. S., Entwistle P. F. 1990; Risk assessment studies – detailed host range testing of wild-type cabbage moth, Mamestra brassicae (Lepidoptera: Noctuidae), nuclear polyhedrosis virus. Applied and Environmental Microbiology 56:2704–2710
    [Google Scholar]
  8. Entwistle P. F., Evans H. F. 1987; Trials on the control of Panolis flammea with a nuclear polyhedrosis virus. In Population Biology and Control of the Pine Beauty Moth (Panolis flammea) Edited by Leather S. R., Stoakley J. T., Evans H. F. Forestry Commission Bulletin 67: pp 61–68
    [Google Scholar]
  9. Faktor O., Toister-Achituv M., Kamensky B. 1995; Identification and nucleotide sequence of an ecdysteroid UDP-glucosyltransferase gene of Spodoptera littoralis multicapsid nuclear polyhedrosis virus. Virus Genes 11:47–52
    [Google Scholar]
  10. Felsenstein J. 1989; PHYLIP - phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  11. Iatrou K., Ito K., Witkiewicz H. 1985; Polyhedrin gene of Bombyx mori nuclear polyhedrosis vims. Journal of Virology 54:436–445
    [Google Scholar]
  12. Kondo A., Maeda S. 1991; Host range expansion by recombination of the baculoviruses Bombyx mori nuclear polyhedrosis vims and Autographa californica nuclear polyhedrosis vims. Journal of Virology 65:3625–3632
    [Google Scholar]
  13. Leisy D., Nesson M., Pearson M., Rohrmann G., Beaudreau G. 1986; Location and nucleotide sequence of the Orgyia pseudotsugata single nucleocapsid nuclear polyhedrosis vims polyhedrin gene. Journal of General Virology 67:1073–1079
    [Google Scholar]
  14. O’Reilly D. R. 1995; Baculovims-encoded ecdysteroid UDP-glucosyl-transferases. Insect Biochemistry and Molecular Biology 25:475–485
    [Google Scholar]
  15. O’Reilly D. R., Miller L. K. 1990; Regulation of expression of a baculovims ecdysteroid UDP-glucosyltransferase gene. Journal of Virology 64:1321–1328
    [Google Scholar]
  16. O’Reilly D. R., Miller L. K. 1991; Improvement of a baculovims pesticide by deletion of the egt gene. Bio/ Technology 9:1086–1089
    [Google Scholar]
  17. O’Reilly D. R., Howarth O. W., Rees H. H., Miller L. K. 1991; Stmcture of the ecdysone glucoside formed by a baculovims ecdysteroid UDP-glucosyltransferase. Insect Biochemistry 21:795–801
    [Google Scholar]
  18. O’Reilly D. R., Brown M. R., Miller L. K. 1992; Alteration of ecdysteroid metabolism due to baculovims infection of the fall armyworm Spodoptera frugiperda: host ecdysteroids are conjugated with galactose. Insect Biochemistry and Molecular Biology 22:313–320
    [Google Scholar]
  19. Payne C. C. 1986; Insect pathogenic viruses as pest control agents. In Biological Plant and Health Protection pp 183–200 Edited by Franz J. M. Stuttgart: Fischer-Verlag;
    [Google Scholar]
  20. Pearson M., Bjornson R., Ahrens C., Rohrmann G. 1993; Identification and characterization of a putative origin of DNA replication in the genome of a baculovims pathogenic for Orgyia pseudotsugata . Virology 197:715–725
    [Google Scholar]
  21. Possee R. D., Kelly D. C. 1988; Physical maps and comparative DNA hybridization of Mamestra brassicae and Panolis flammea nuclear polyhedrosis vims genomes. Journal of General Virology 69:1285–1298
    [Google Scholar]
  22. Riegel C. I., Lanner-Herrera C., Slavicek J. M. 1994; Identification and characterization of the ecdysteroid UDP-glucosyltransferase gene of the Lymantria dispar multinucleocapsid nuclear polyhedrosis vims. Journal of General Virology 75:829–838
    [Google Scholar]
  23. Smith I. R., Van Beek N. A., Podgwaite J. D., Wood H. A. 1988; Physical map and polyhedrin gene sequence of lymantria dispar nuclear polyhedrosis virus. Gene 71:97–105
    [Google Scholar]
  24. Swofford D. L. 1991; PAUP: phylogenetic analysis using parsimony (version 3.1). Computer program distributed by the Illinois Natural History Survey, Champaign, Illinois, USA
    [Google Scholar]
  25. Zanotto P. M., Kessing B. D., Maruniak J. E. 1993; Phylogenetic interrelationships among baculoviruses: evolutionary rates and host associations. Journal of Invertebrate Pathology 62:147–164
    [Google Scholar]
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