1887

Abstract

Four DNA-dependent ATPases were purified from vaccinia virions and tested for DNA helicase activity on two dsDNA substrates. ATPases D6R and D11L were inactive on both substrates, A18R unwound the substrate with a short 20 bp duplex region and 18R unwound both substrates. In addition, the I8R protein was stimulated to unwind longer DNA duplexes by a 25 kDa protein purified from vaccinia virions, representing the cleaved product of the L4R gene, an ssDNA binding protein. Purified recombinant 25 kDa L4R protein also stimulated I8R DNA helicase activity and maximum activity was observed only when there were < 13 nucleotides of DNA per molecule of L4R protein. The DNA helicase activity of the A18R protein was not stimulated by either recombinant 25 kDa L4R protein or by an ssDNA binding protein.

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1996-11-01
2024-12-07
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References

  1. Bayliss C. D., Condit R. C. 1995; The vaccinia virus A18R gene product is a DNA-dependent ATPase. Journal of Biological Chemistry 270:1550–1556
    [Google Scholar]
  2. Bayliss C. D., Smith G. L. 1996; Vaccinia virion protein 18R has both DNA and RNA helicase activities: implications for vaccinia virus transcription. Journal of Virology 70:794–800
    [Google Scholar]
  3. Broyles S. S., Moss B. 1987; Identification of the vaccinia virus gene encoding nucleoside triphosphate phosphohydrolase 1, a DNA-dependent ATPase. Journal of Virology 61:1738–1742
    [Google Scholar]
  4. Broyles S. S., Moss B. 1988; DNA-dependent ATPase activity associated with vaccinia virus early transcription factor. Journal of Biological Chemistry 263:10761–10765
    [Google Scholar]
  5. Fathi Z., Condit R. C. 1991; Phenotypic characterization of a vaccinia virus temperature-sensitive complementation group affecting a virion component. Virology 181:273–276
    [Google Scholar]
  6. Gershon P. D., Moss B. 1990; Early transcription factor subunits are encoded by vaccinia virus late genes. Proceedings of the National Academy of Sciences, USA 87:4401–4405
    [Google Scholar]
  7. Gross C. H., Shuman S. 1996; Vaccinia virus RNA helicase: nucleic acid specificity in duplex unwinding. Journal of Virology 70:2615–2619
    [Google Scholar]
  8. Kao S. Y., Ressner E., Kates J., Bauer W. R. 1981; Purification and characterization of a superhelix binding protein from vaccinia virus. Virology 111:500–508
    [Google Scholar]
  9. Koonin E. V., Senkevich T. G. 1992; Vaccinia virus encodes four putative DNA and/or RNA helicases distantly related to each other. Journal of General Virology 73:989–993
    [Google Scholar]
  10. Paoletti E., Rosemond-Hornbeak H., Moss B. 1974; Two nucleic acid-dependent nucleoside triphosphate phosphohydrolases from vaccinia virus. Purification and characterization. Journal of Biological Chemistry 249:3273–3280
    [Google Scholar]
  11. Rodriguez J. F., Kahn J. S., Esteban M. 1986; Molecular cloning, encoding sequence, and expression of vaccinia virus nucleic acid-dependent nucleoside triphosphatase. gene 83:9566–9570
    [Google Scholar]
  12. Shuman S. 1992; Vaccinia virus RNA helicase: an essential enzyme related to the DE-H family of RNA-dependent NTPases. Proceedings of the National Academy of Sciences, USA 89:10935–10939
    [Google Scholar]
  13. Simpson D. A., Condit R. C. 1995; Vaccinia virus gene A18R encodes an essential DNA helicase. Journal of Virology 69:6131–6139
    [Google Scholar]
  14. Weir J. P., Moss B. 1984; Regulation of expression and nucleotide sequence of a late vaccinia virus gene. Journal of Virology 51:662–669
    [Google Scholar]
  15. Wilcock D., Smith G. L. 1994; Vaccinia virus core protein VP8 is required for virus infectivity, but not for core protein processing or for INV and EEV formation. Virology 202:294–304
    [Google Scholar]
  16. Wilcock D., Smith G. L. 1996; Vaccinia virions lacking core protein VP8 are deficient in early transcription. Journal of Virology 70:934–943
    [Google Scholar]
  17. Yang W. P., Bauer W. R. 1988; Purification and characterization of vaccinia virus structural protein VP8. Virology 167:578–584
    [Google Scholar]
  18. Yang W. P., Kao S. Y., Bauer W. R. 1988; Biosynthesis and posttranslational cleavage of vaccinia virus structural protein VP8. Virology 167:585–590
    [Google Scholar]
  19. Zhang Y., Moss B. 1991; Vaccinia virus morphogenesis is interrupted when expression of the gene encoding an 11-kilodalton phosphorylated protein is prevented by the Escherichia coli lac repressor. Journal of Virology 65:6101–6110
    [Google Scholar]
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