RT Journal Article SR Electronic(1) A1 Blewett, Earl Linwood A1 Black, Darla A1 Eberle, R.YR 1996 T1 Characterization of virus-specific and cross-reactive monoclonal antibodies to Herpesvirus simiae (B virus) JF Journal of General Virology, VO 77 IS 11 SP 2787 OP 2793 DO https://doi.org/10.1099/0022-1317-77-11-2787 PB Microbiology Society, SN 1465-2099, AB A panel of 13 monoclonal antibodies (MAbs) was produced that detect B virus (BV) proteins. Several of these MAbs were highly specific for BV, while the remainder cross-reacted in varying degrees with the other primate alphaherpesviruses. Utilizing western blot and radioimmunoprecipitation analysis, the MAbs were found to detect at least four distinct BV-infected cell antigens, several of which were composed of multiple polypeptides. One target antigen has been identified as the BV glycoprotein B (gB) homologue and was recognized by both virus-specific and cross-reactive MAbs. Although gB is an essential protein, none of the anti-gB MAbs neutralized infectious virus., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-77-11-2787