1887

Abstract

Following intracerebral inoculation of the BeAn strain of Theiler’s murine encephalomyelitis virus the course of the acute infection and persistence of virus in the CNS varies between individual CBA mice. On the basis of clinical signs, virus distribution, virus titres, histopathology and Southern blot hybridization analysis of virus specific RT-PCR amplified products from total brain and spinal cord RNA, individual CBA mice could be placed into one of three groups. The first group were those animals which died of acute encephalitis. The second group were animals with or without clinical signs which had early high CNS virus titres, and in addition to scattered foci of infection had spread of virus in specific neuronal nuclei followed by destruction of these areas and thereafter persistence of virus in the CNS. The third group had no clinical signs, low CNS virus titres, small foci of CNS infection and were negative for virus after 28 days. This third pattern of infection was also seen in BALB/c mice. Between 50 and 268 days post-infection 53% of CBA mice were positive for viral RNA in the CNS by RT-PCR. No BALB/c mice were positive. In both the acute and persistent infection there was a correlation between serum neutralizing antibody titre and clinical signs. During the acute infection BeAn RNA could be detected in neurons and astrocytes. Oligodendrocytes were negative. In those CBA mice with persistence, viral RNA was observed in scattered individual or small foci of cells, predominantly oligodendrocytes, in both the brain and spinal cord.

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1996-11-01
2022-08-17
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References

  1. Aubert C., Chamorro M., Brahic M. 1987; Identification of Theiler’s virus infected cells in the central nervous system of the mouse during demyelinating disease. Microbial Pathogenesis 3:319–326
    [Google Scholar]
  2. Azoulay A., Brahic M., Bureau J. F. 1994; FVB mice transgenic for the H-2Db gene become resistant to persistent infection by Theiler’s virus. Journal of Virology 68:4049–4052
    [Google Scholar]
  3. Blakemore W. F., Welsh C. J., Tonks P., Nash A. A. 1988; Observations on demyelinating lesions induced by Theiler′s virus in CBA mice. Acta Neuropathologica 76:581–589
    [Google Scholar]
  4. Borrow P., Welsh C. J. R., Nash A. A. 1993; Study of the mechanisms by which CD4 + T cells contribute to protection in Theiler’s murine encephalomyelitis. Immunology 80:502–506
    [Google Scholar]
  5. Brahic M., Stroop W. G., Baringer J. R. 1981; Theiler’s virus persists in glial cells during demyelinating disease. Cell 26:123–128
    [Google Scholar]
  6. Bureau J. F., Montagutelli X., Lefebvre S., Guenet J. L., Pla M., Brahic M. 1992; The interaction of two groups of murine genes determines the persistence of Theiler’s virus in the central nervous system. Journal of Virology 66:4698–4704
    [Google Scholar]
  7. Bureau J. F., Montagutelli X., Bihl F., Lefebvre S., Guenet J. L., Brahic M. 1993; Mapping loci influencing the persistence of Theiler’s virus in the murine central nervous system. Nature Genetics 5:87–91
    [Google Scholar]
  8. Cash E., Chamorro M., Brahic M. 1985; Theiler′s virus RNA and protein synthesis in the central nervous system of demyelinating mice. Virology 144:290–294
    [Google Scholar]
  9. Cash E., Chamorro M., Brahic M. 1986; Quantitation, with a new assay, of Theiler’s virus capsid protein in the central nervous system of mice. Journal of Virology 60:558–563
    [Google Scholar]
  10. Cash E., Chamorro M., Brahic M. 1988; Minus-strand RNA synthesis in the spinal cords of mice persistently infected with Theiler’s virus. Journal of Virology 62:1824–1826
    [Google Scholar]
  11. Chamorro M., Aubert C., Brahic M. 1986; Demyelinating lesions due to Theiler′s virus are associated with ongoing central nervous system infection. Journal of Virology 57:992–997
    [Google Scholar]
  12. Chomczynski P., Sacchi N. 1987; Single step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  13. Clatch R. J., Melvold R. W., Miller S. D., Lipton H. L. 1985; Theiler′s murine encephalomyelitis virus (TMEV)-induced demyelinating disease in mice is influenced by the H-2D region: correlation with TEMV-specific delayed-type hypersensitivity. Journal of Immunology 135:1408–1414
    [Google Scholar]
  14. Clatch R. J., Melvold R. W., Dal Canto M. C., Miller S. D., Lipton H. L. 1987; The Theiler’s murine encephalomyelitis virus (TMEV) model for multiple sclerosis shows a strong influence of the murine equivalents of HLA-A, B, and C. Journal of Neuroimmunology 15:121–135
    [Google Scholar]
  15. Fazakerley J. K., Pathak S., Scallan M., Amor S., Dyson H. 1993; Replication of the A7(74) strain of Semliki Forest virus is restricted in neurons. Virology 195:627–637
    [Google Scholar]
  16. Fiette L., Aubert C., Brahic M., Pena Rossi C. 1993; Theiler’s virus infection of β 2-microglobulin-deficient mice. Journal of Virology 67:589–592
    [Google Scholar]
  17. Fishman P. S., Gass J. S., Swoveland P. T., Lavi E., Highkin M. K., Weiss S. R. 1985; Infection of the basal ganglia by a murine coronavirus. Science 229:877–879
    [Google Scholar]
  18. Fujinami R. S., Rosenthal A., Lampert P. W., Zurbriggen A., Yamada M. 1989; Survival of athymic (nu/nu) mice after Theiler’s murine encephalomyelitis virus infection by passive administration of neutralising monoclonal antibody. Journal of Virology 62:2081–2087
    [Google Scholar]
  19. Kurtz C. I. B., Sun X. I., Fujinami R. S. 1995; B-lymphocyte requirement for vaccine-mediated protection from Theiler’s murine encephalomyelitis virus-induced central nervous system disease. Journal of Virology 69:5152–5155
    [Google Scholar]
  20. Lavi E., Fishman P. S., Highkin M. K., Weiss S. R. 1988; Limbic encephalitis after inhalation of a murine coronavirus. Laboratory Investigation 58:31–36
    [Google Scholar]
  21. Lindsley M. D., Rodriguez M. 1989; Characterization of the inflammatory response in the central nervous system of mice susceptible or resistant to demyelination by Theiler′s virus. Journal of Immunology 142:2677–2682
    [Google Scholar]
  22. Lindsley M. D., Thiemann R., Rodriguez M. 1991; Cytotoxic T cells isolated from the central nervous system of mice infected with Theiler′s virus. Journal of Immunology 65:6612–6620
    [Google Scholar]
  23. Lipton H. L. 1975; Theiler′s virus infection in mice. An unusual biphasic disease process leading to demyelination. Infection and Immunity 11:1147–1155
    [Google Scholar]
  24. Lipton H. L., Kratochvil J., Sethi P., Dal Canto M. C. 1984; Theiler′s virus antigen detected in mouse spinal cord years after infection. Neurology 34:1117
    [Google Scholar]
  25. Love S. 1987; Distribution of Theiler′s virus in the CNS of athymic nude mice: effect of varying the route of inoculation. Journal of Neurological Science 81:55–66
    [Google Scholar]
  26. Maniatis T., Fritsch E. F., Sambrook J. 1989 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. McPhee I. A., Antoni F. A., Mason D. W. 1989; Spontaneous recovery of rats from experimental allergic encephalomyelitis is dependent on regulation of the immune system by endogenous adrenal corticosteroids. Journal of Experimental Medicine 169:431–445
    [Google Scholar]
  28. Melvold R. W., Jokinen D. M., Knobler R. L., Lipton H. L. 1987; Variations in genetic control of susceptibility to Theiler’s murine encephalomyelitis virus (TMEV)-induced demyelinating disease. Journal of Immunology 138:1429–1433
    [Google Scholar]
  29. Melvold R. W., Jokinen D. M., Miller S. D., Dal Canto M. C., Lipton H. L. 1990; Identification of a locus on mouse chromosome 3 involved in differential susceptibility to Theiler′s murine encephalomyelitis virus-induced demyelinating disease. Journal of Virology 64:686–690
    [Google Scholar]
  30. Olitsky P. K. 1939; Viral effect produced by intestinal contents of normal mice and those having spontaneous encephalomyelitis. Proceedings of the Society for Experimental Biology and Medicine 41:434–437
    [Google Scholar]
  31. Ozden S., Tangy F., Chamorro M., Brahic M. 1986; Theiler′s virus genome is closely related to that of encephalomyocarditis virus, the prototype cardiovirus. Journal of Virology 60:1163–1165
    [Google Scholar]
  32. Patick A. K., Oleszak E. L., Leibowitz J. L., Rodriguez M. 1990; Persistent infection of a glioma cell line generates a Theiler’s virus variant which fails to induce demyelinating disease in SJL/J mice. Journal of General Virology 71:212–219
    [Google Scholar]
  33. Pena Rossi C., McAllister A., Fiette L., Brahic M. 1991; Theiler’s virus infection induces a specific cytotoxic T lymphocyte response. Cellular Immunology 138:341–348
    [Google Scholar]
  34. Perlman S., Jacobsen G., Moore S. 1988; Regional localization of virus in the central nervous system of mice persistently infected with murine coronavirus JHM. Virology 170:556–560
    [Google Scholar]
  35. Peterson J. D., Karpus W. J., Clatch R. J., Miller S. D. 1993; Split tolerance of Th1 and Th2 cells in tolerance to Theiler′s murine encephalomyelitis virus. European Journal of Immunology 23:46–55
    [Google Scholar]
  36. Pevear D. C., Calenoff M., Rozhon E., Lipton H. L. 1987; Analysis of the complete nucleotide sequence of the picomavirus Theiler’s murine encephalomyelitis virus indicates that it is closely related to cardioviruses. Journal of Virology 61:1507–1516
    [Google Scholar]
  37. Pullen L. C., Miller S. D., Dal Canto M. C., Kim B. S. 1993; Class I-deficient resistant mice intracerebrally inoculated with Theiler’s virus show an increased T cell response to viral antigens and susceptibility to demyelination. European Journal of Immunology 23:2287–2293
    [Google Scholar]
  38. Raible D. W., McMorris F. A. 1989; Cyclic AMP regulates the rate of differentiation of oligodendrocytes without changing the lineage commitment of their progenitors. Developmental Biology 133:437–446
    [Google Scholar]
  39. Rodriguez M., David C. S. 1985; Demyelination induced by Theiler’s virus: influence of the H-2 haplotype. Journal of Immunology 135:2145–2148
    [Google Scholar]
  40. Rodriguez M., Leibowitz J., David C. S. 1986; Susceptibility to Theiler’s virus-induced demyelination. Mapping of the gene within the H-2D region. Journal of Experimental Medicine 163:620–631
    [Google Scholar]
  41. Rodriguez M., Kenny J. J., Thiemann R. L., Woloschak G. E. 1990; Theiler’s virus-induced demyelination in mice immuno-suppressed with anti-IgM and in mice expressing the xid gene. Microbial Pathogenesis 8:23–35
    [Google Scholar]
  42. Rodriguez M., Pedrinaci S., David C. S. 1993; Abrogation of resistance to Theiler′s virus induced demyelination in H-2b mice deficient in β-microglobulin. Journal of Immunology 151:266–276
    [Google Scholar]
  43. Rueckert R. R., Pallanch M. A. 1981; Preparation and characterization of encephalomyocarditis (EMC) virus. Methods in Enzymology 78:315–325
    [Google Scholar]
  44. Simas J. P., Dyson H., Fazakerley J. K. 1995; The neurovirulent GDVII strain of TMEV can replicate in glial cells. Journal of Virology 69:5599–5606
    [Google Scholar]
  45. Theiler M. 1934; Spontaneous encephalomyelitis of mice – a new virus disease. Science 80:122–123
    [Google Scholar]
  46. Wada Y., Fujinami R. S. 1993; Viral infection and dissemination through the olfactory pathway and the limbic system by Theiler′s virus. American Journal of Pathology 143:221–229
    [Google Scholar]
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