1887

Journal of General Virology header logo

Volume 77, Issue 10

Dengue 1 virus binding to human hepatoma HepG2 and simian Vero cell surfaces differs Free

Abstract

We analysed the binding and infectivity of dengue virus serotype 1 (DEN-1) for the human hepatoma cell line HepG2 in comparison with the simian kidney cell line Vero. The higher susceptibility of Vero cells to DEN-1 correlated with greater binding affinity of DEN-1 to these cells. In contrast, the capacity of virus attachment was higher for HepG2 than for Vero cells. Profiles of DEN-1 binding at different pH were markedly different between the two cell types. A type-specific neutralizing monoclonal antibody reduced initial virus binding to both cell types similarly but complex- and group-specific neutralizing antibodies affected virus adhesion differently. Altogether, these results suggest the involvement of different receptors or receptors presented in a different environment on the cell surface in the two cell lines. The sensitivity to proteolytic enzymes and to ionic detergent of the binding sites on the two cell types was tested and results indicated that they may be multimeric proteins or protein complexes.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1996
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-10-2547
1996-10-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/10/JV0770102547.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-10-2547&mimeType=html&fmt=ahah

References

  1. Anderson R., King A. D., Innis B. L. 1992; Correlation of E protein binding with cell susceptibility to dengue 4 virus infection. Journal of General Virology 73:2155–2159
     [Google Scholar]
  2. Bhamarapravati N., Boonyapaknavik V. 1966; Pathogenic studies on Thai haemorrhagic fever: immunofluorescence localisation of dengue virus in human tissue. Bulletin of the World Health Organization 35:50–51
     [Google Scholar]
  3. Bhamarapravati N., Toochinda P., Boonyapaknavik V. 1967; Pathology of Thailand haemorrhagic fever: a study of 100 autopsy cases. American Journal of Tropical Medicine and Parasitology 61:500–510
     [Google Scholar]
  4. Burke T. 1968; Dengue haemorrhagic fever: a pathological study. Transaction of the Royal Society of Tropical Medicine and Hygiene 62:682–692
     [Google Scholar]
  5. Chungue E., Cassar O., Drouet M. T., Guzman M. G., Laille M., Rosen L., Deubel V. 1995; Molecular epidemiology of dengue-1 and dengue-4 viruses. Journal of General Virology 76:1877–1884
     [Google Scholar]
  6. Compton S. R., Stephensen C. B., Snyder S. W., Weismiller D. G., Holmes K. V. 1992; Coronavirus species specificity: murine corona-vims binds to a mouse-specific epitope on its carcinoembryonic antigen-related receptor glycoprotein. Journal of Virology 66:7420–7428
     [Google Scholar]
  7. Daughaday C. C., Brandt W. E., McCown J. M., Russell P. K. 1981; Evidence for two mechanisms of dengue vims infection of adherent human monocytes: trypsin-sensitive vims receptors and trypsin-resistant immune complex receptors. Infection and Immunity 32:469–473
     [Google Scholar]
  8. Desprès P., Frenkiel M. P., Deubel V. 1993; Differences between cell membrane fusion activities of two dengue type-1 isolates reflect modifications of viral stmcture. Virology 196:209–219
     [Google Scholar]
  9. Fresh J. W., Reyes V., Clarke E. J., Uylangco C. V. 1969; Philippine hemorrhagic fever: a clinical, laboratory, and necropsy study. Journal of Laboratory and Clinical Medicine 73:451–458
     [Google Scholar]
  10. Gollins S. W., Porterfield J. S. 1985; Flavivims infection enhancement in macrophages: an electron microscopic study of viral cellular entry. Journal of General Virology 66:1969–1982
     [Google Scholar]
  11. Gollins S. W., Porterfield J. S. 1986; A new mechanism for the neutralization of enveloped viruses by antiviral antibody. Nature 321:244–246
     [Google Scholar]
  12. Guirakhoo F., Heinz F. X., Mandl C. W., Holzmann H., Kunz C. 1991; Fusion activity of flaviviruses: comparison of mature and immature (prM-containing) tick-borne encephalitis virions. Journal of General Virology 72:1323–1329
     [Google Scholar]
  13. Guirakhoo F., Hunt A. R., Lewis J. G., Roehrig J. T. 1993; Selection and partial characterization of dengue 2 virus mutants that induce fusion at elevated pH. Virology 194:219–223
     [Google Scholar]
  14. Hall W. C., Crowell T. P., Watts D. M., Barros V. L. R., Kruger H., Pinheiro F., Peters C. J. 1991; Demonstration of yellow fever and dengue antigens in formalin-fixed paraffin-embedded human liver by immunohistological analysis. American Journal of Tropical Medicine and Hygiene 45:408–417
     [Google Scholar]
  15. Halstead S. B. 1979; In vivo enhancement of dengue virus infection in rhesus monkeys by passively transferred antibody. Journal of Infectious Diseases 140:527–533
     [Google Scholar]
  16. Halstead S. B. 1994; Antibody-dependent enhancement of infection: a mechanism for indirect virus entry into cells. In Cellular Receptors for Animal Viruses pp 493–516 Edited by Wimmer E. New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  17. Innis B. L. 1995; Dengue and dengue hemorrhagic fever. In Exotic Viral Infections pp 103–146 Edited by Porterfield J. S. New York & London: Chapman and Hall;
     [Google Scholar]
  18. Jin Y. M., Pardoe I. U., Burness A. T., Michalak T. I. 1994; Identification and characterization of the cell surface 70-kilodalton sialoglycoprotein(s) as a candidate receptor for encephalomyocarditis virus on human nucleated cells. Journal of Virology 68:7308–7319
     [Google Scholar]
  19. Kangwanpong D., Bhamarapravati N., Lucia H. L. 1995; Diagnosing dengue virus infection in archived autopsy tissues by means of the in situ PCR method: a case report. Clinical and Diagnostic Virology 3:165–172
     [Google Scholar]
  20. Kimura T., Kimura K. J., Nagashima K., Yasui K. 1994; Analysis of virus-cell binding characteristics on the determination of Japanese encephalitis virus susceptibility. Archives of Virology 139:239–251
     [Google Scholar]
  21. Kuno G., Gubler D. G., Velez M., Oliver A. 1985; Comparative sensitivity of three mosquito cell lines for isolation of dengue viruses. Bulletin of the World Health Organization 63:279–286
     [Google Scholar]
  22. Kuo C.-H., Tai D.-I., Chang-Chien C.-S., Lan C.-K., Chiou S.-S., Liaw Y.-F. 1992; Liver biochemical tests and dengue fever. American Journal of Tropical Medicine and Hygiene 47:265–270
     [Google Scholar]
  23. Lentz T. L. 1990; The recognition event between virus and host cell receptor: a target for antiviral agents. Journal of General Virology 71:751–766
     [Google Scholar]
  24. Monath T. P. 1990; Flaviviruses. In Virology 2nd edn pp 763–814 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
     [Google Scholar]
  25. Monath T. P. 1994; Yellow fever and dengue: the interactions of virus, vector and host in the re-emergence of epidemic disease. Seminars in Virology 5:133–145
     [Google Scholar]
  26. Morens D. M., Halstead S. B. 1990; Measurement of antibody-dependent infection enhancement of four dengue virus serotypes by monoclonal and polyclonal antibodies. Journal of General Virology 71:2909–2914
     [Google Scholar]
  27. Morens D. M., Halstead S. B., Larsen L. K. 1985; Comparison of dengue virus plaque reduction neutralization by macro and ‘semi-micro’ methods in LLC-MK2 cells. Microbiology and Immunology 29:1197–1205
     [Google Scholar]
  28. Morens D. M., Venkateshan C. N., Halstead S. B. 1987; Dengue 4 virus monoclonal antibodies identify epitopes that mediate immune infection enhancement of dengue 2 viruses. Journal of General Virology 68:91–98
     [Google Scholar]
  29. Rey F., Heinz F. X., Mandl C., Kunz C., Harrison S. C. 1995; The envelope glycoprotein from tick-borne encephalitis virus at 2A resolution. Nature 375:291–298
     [Google Scholar]
  30. Roivainen M., Piirainen L., Hovi T., Virtanen I., Riikonen T., Heino J., Hyypia T. 1994; Entry of coxsackievirus A9 into host cells: specific interactions with alpha v beta 3 integrin, the vitronectin receptor. Virology 203:357–365
     [Google Scholar]
  31. Rosen L., Khin M. M., Tin U. 1989; Recovery of vims from the liver of children with fatal dengue: reflections on the pathogenesis of the disease and its possible analogy with that of yellow fever. Research in Virology 140:351–360
     [Google Scholar]
  32. Schlesinger R. W. 1977 Dengue Viruses New York: Springer-Verlag;
     [Google Scholar]
  33. Scott R. M., Nisalak A., Cheamudon U., Seridhoranakul S., Nimmannitya S. 1980; Isolation of dengue vimses from peripheral blood leukocytes of patients with hemorrhagic fever. Journal of Infectious Diseases 141:1–6
     [Google Scholar]
  34. Smith A. L., Tignor G. H. 1980; Host cell receptors for two strains of Sindbis virus. Archives of Virology 66:11–26
     [Google Scholar]
  35. Strauss J. H., Wang K. S., Schmaljohn A. L., Kuhn R. J., Strauss E. G. 1994; Host-cell receptors for Sindbis virus. Archives of Virology 9:473–484
     [Google Scholar]
  36. Tucker P. C., Griffin D. E. 1991; Mechanism of altered Sindbis vims neurovirulence associated with a single-amino-acid change in the E2 glycoprotein. Journal of Virology 65:1551–1557
     [Google Scholar]
  37. Ubol S., Griffin D. E. 1991; Identification of a putative alphavirus receptor on mouse neural cells. Journal of Virology 65:6913–6921
     [Google Scholar]
  38. Wengler G., Wengler G. 1989; Cell-associated West Nile flavivirus is covered with E + Pre-M protein heterodimers which are destroyed and reorganized by proteolytic cleavage during vims release. Journal of Virology 63:2521–2526
     [Google Scholar]
  39. Yoksan B., Bhamarapravati N. 1983; Localization of dengue antigens in tissue specimens from fatal cases of dengue haemorrhagic fever. In Proceedings of the International Conference on Dengue/Dengue Haemorrhagic Fever pp 406–410 Edited by Pang R. P. T. University of Malaya; Kuala Lumpur, Malaysia:
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-10-2547
Loading
Dengue 1 virus binding to human hepatoma HepG2 and simian Vero cell surfaces differs
J. Gen. Virol. 77, 2547 (1996); https://doi.org/10.1099/0022-1317-77-10-2547
/content/journal/jgv/10.1099/0022-1317-77-10-2547
/content/journal/jgv/10.1099/0022-1317-77-10-2547
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error