1887

Abstract

We have tested whether immunity can be transferred from a mother fish to its fry. Rainbow trout mother fish were inoculated against infectious haematopoietic necrosis virus (IHNV) by intraperitoneal injection of a fragment of the IHNV glycoprotein spanning amino acids 31 to 310. This protein fragment was obtained by isolating the specific cDNA from Japanese IHNV strain HV7601 and expressing it in . Fry from immunized and control fish were exposed to IHNV at various intervals after hatching, and their mortality monitored. Survival of the fry of immunized fish was significantly greater when exposure to virus occurred 7 days after hatching, and some immunity appeared to persist until at least 25 days after hatching.

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1996-10-01
2024-07-16
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References

  1. Amend D. F. 1975; Detection and transmission of infectious hematopoietic necrosis virus in rainbow trout. Journal of Wildlife Disease 11:471–480
    [Google Scholar]
  2. Amend D. F., Fender D. C. 1976; Uptake of bovine serum albumin by rainbow trout from hyperosmotic solution: a model for vaccinating fish. Science 192:793–794
    [Google Scholar]
  3. Avtalion R., Mor A. 1990; Transfer of antibody activity from immunized mother to embryo in tilapias. Journal of Fish Biology 37:249–255
    [Google Scholar]
  4. Bly J. E., Grimm A. S., Morris I. G. 1986; Transfer of passive immunity from mother to a young teleost fish: haemagglutinating activity in the serum and eggs of plaice, Pleuronectes platessa L . Comparative Biochemistry and Physiology 84A:309–313
    [Google Scholar]
  5. Castillo C., Sanchez C., Dominguez J., Kaattari S., Viilena A. 1993; Ontogeny of IgM and IgM-bearing cells in rainbow trout. Developmental and Comparative Immunology 17:419–424
    [Google Scholar]
  6. Engelking H. M., Leong J. C. 1989; The glycoprotein of infectious hematopoietic necrosis virus elicits neutralizing antibody and protective response. Virus Research 13:213–230
    [Google Scholar]
  7. Gilmore R. D., Engelking H. M., Manning D. S., Leong J. C. 1988; Expression of E. coli of complementary DNA fragment encoding an immunogenic determinant of the glycoprotein of infectious hematopoietic necrosis virus. Bio/Technology 6:295–300
    [Google Scholar]
  8. Johnson K. A., Flynn J. K., Amend D. F. 1982; Onset of immunity in salmonid fry vaccinated by direct immersion in Vibrio anguillarum and Yersinia ruckeri bacterins. Journal of Fish Disease 5:197–205
    [Google Scholar]
  9. Kamei Y., Yoshimizu M., Kimura T. 1987; Plaque assay of Onchorhynchus masou virus (OMV). Fish Pathology 22:147–152
    [Google Scholar]
  10. Kasai K., Yonezawa J., Ono A., Hasegawa A., Honma T., Fukuda H. 1993; Brood and size dependent variation in susceptibility of rainbow trout, Oncorhynchus mykiss to artificial infection of Infectious Hematopoietic Necrosis virus. Fish Pathology 28:35–40
    [Google Scholar]
  11. Koener J. F., Passavant C. W., Kurath G., Leong J. C. 1987; Nucleotide sequence of a cDNA clone carrying the glycoprotein of infectious hematopoietic necrosis virus, a fish rhabdovirus. Journal of Virology 61:1342–1349
    [Google Scholar]
  12. Kurath G., Leong J. C. 1985; Characterization of infectious hematopoietic necrosis virus mRNA species reveals a nonvirion rhabdovirus protein. Journal of Virology 53:462–468
    [Google Scholar]
  13. Kurath G., Ahern K. G., Pearson G. D., Leong J. C. 1985; Molecular cloning of the six mRNA species of infectious hematopoietic necrosis virus, a fish rhabdovirus, and gene order determination by R-loop mapping. Journal of Virology 53:469–476
    [Google Scholar]
  14. McAllister P. E., Wagner R. R. 1975; Structural proteins of two salminid rhabdoviruses. Journal of Virology 15:733–738
    [Google Scholar]
  15. Mosmann T. 1983; Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65:55–63
    [Google Scholar]
  16. Ristow S. S., Avila J., LaPatra S. E., Lauda K. 1993; Detection and characterization of rainbow trout antibody against infectious hematopoietic necrosis virus. Disease of Aquatic Organisms 15:109–114
    [Google Scholar]
  17. Smith D. B., Johnson K. S. 1988; Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31–40
    [Google Scholar]
  18. Xu L., Mourich D. V., Engelking H. M., Ristow S., Arnzen J., Leong J. C. 1991; Epitope mapping and characterization of the infectious hematopoietic necrosis virus glycoprotein, using fusion protein synthesized in Escherichia coli . Journal of Virology 65:1611–1615
    [Google Scholar]
  19. Yamada S., Hata J.-I., Yamashita S. 1993; Molecular cloning of fish Pit-1 cDNA and its functional binding to promoter of gene expressed in the pituitary. Journal of Biological Chemistry 268:24361–24366
    [Google Scholar]
  20. Yamashita S., Wada K., Horikoshi M., Gong D.-W., Kokubo T., Hisatake K., Yokotani N., Malik S., Roeder R. G., Nakatani Y. 1992; Isolation and characterization of a cDNA encoding Drosophila transcription factor TFIIB . Proceedings of the National Academy of Sciences, USA 89:2839–2843
    [Google Scholar]
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