RT Journal Article SR Electronic(1) A1 Jin, Minghao A1 Ido, Eiji A1 Kuwata, Takeo A1 Igarashi, Tatsuhiko A1 Cichutek, Klaus A1 Kurth, Reinhard A1 Miura, Tomoyuki A1 Enose, Yoshimi A1 Chen, Jiangli A1 Hayami, MasanoriYR 1996 T1 Replication and cytopathogenicity of human immunodeficiency virus type 1 (HIV-1)/simian immunodeficiency virus agm3 chimeric viruses in human and monkey cells: the 5′ half of the HIV-1 genome is responsible for virus cytopathogenicity JF Journal of General Virology, VO 77 IS 10 SP 2427 OP 2431 DO https://doi.org/10.1099/0022-1317-77-10-2427 PB Microbiology Society, SN 1465-2099, AB Two chimeric viruses were constructed between human immunodeficiency virus type 1 (HIV-1) and an apathogenic simian immunodeficiency virus (SIVagm3mc) from African green monkeys. One of the chimeras, HE-A391, expressed the HIV-1-derived env, vpu, tat and rev genes and the SIVagm3mc-derived LTR and the gag, pol and vif genes. The other chimera, SE-H13, contained the SIVagm3mc-derived env, tat and rev genes and the HIV-1-derived LTR and the gag, pol, vif and nef genes. Both constructs yielded infectious viruses and their phenotypes (growth-competence and cell-killing capacity) were examined in various CD4+ cells including human and monkey PBMCs. The results indicated that the replicative properties of the chimeras were mainly dependent on the 5′-genomic half of the parental viruses, and the determinant for viral cytopathogenicity was located within the 5′ half of the HIV-1 genome., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-77-10-2427