@article{mbs:/content/journal/jgv/10.1099/0022-1317-77-10-2427, author = "Jin, Minghao and Ido, Eiji and Kuwata, Takeo and Igarashi, Tatsuhiko and Cichutek, Klaus and Kurth, Reinhard and Miura, Tomoyuki and Enose, Yoshimi and Chen, Jiangli and Hayami, Masanori", title = "Replication and cytopathogenicity of human immunodeficiency virus type 1 (HIV-1)/simian immunodeficiency virus agm3 chimeric viruses in human and monkey cells: the 5′ half of the HIV-1 genome is responsible for virus cytopathogenicity", journal= "Journal of General Virology", year = "1996", volume = "77", number = "10", pages = "2427-2431", doi = "https://doi.org/10.1099/0022-1317-77-10-2427", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-77-10-2427", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Two chimeric viruses were constructed between human immunodeficiency virus type 1 (HIV-1) and an apathogenic simian immunodeficiency virus (SIVagm3mc) from African green monkeys. One of the chimeras, HE-A391, expressed the HIV-1-derived env, vpu, tat and rev genes and the SIVagm3mc-derived LTR and the gag, pol and vif genes. The other chimera, SE-H13, contained the SIVagm3mc-derived env, tat and rev genes and the HIV-1-derived LTR and the gag, pol, vif and nef genes. Both constructs yielded infectious viruses and their phenotypes (growth-competence and cell-killing capacity) were examined in various CD4+ cells including human and monkey PBMCs. The results indicated that the replicative properties of the chimeras were mainly dependent on the 5′-genomic half of the parental viruses, and the determinant for viral cytopathogenicity was located within the 5′ half of the HIV-1 genome.", }