Two chimeric viruses were constructed between human immunodeficiency virus type 1 (HIV-1) and an apathogenic simian immunodeficiency virus (SIVagm3mc) from African green monkeys. One of the chimeras, HE-A391, expressed the HIV-1-derived and genes and the SIVagm3mc-derived LTR and the and genes. The other chimera, SE-H13, contained the SIVagm3mc-derived and genes and the HIV-1-derived LTR and the and genes. Both constructs yielded infectious viruses and their phenotypes (growth-competence and cell-killing capacity) were examined in various CD4 cells including human and monkey PBMCs. The results indicated that the replicative properties of the chimeras were mainly dependent on the 5′-genomic half of the parental viruses, and the determinant for viral cytopathogenicity was located within the 5′ half of the HIV-1 genome.


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