Variation of viroid profiles in individual grapevine plants: novel grapevine yellow speckle viroid 1 mutants show alterations of hairpin I Free

Abstract

This is the first report which gives a general survey about viroid variant composition in a vineyard and within single plants. A German vineyard with 20-year-old grapevines () of the cultivars ‘Bacchus’ and ‘Kerner’ was analysed for viroid infections. Only grapevine yellow speckle viroid 1 (GYSVd1) and the grapevine isolate of hop stunt viroid (HSVdg) were detected. Both viroids occur in several sequence variations. Eighteen novel GYSVd1 variants and two previously published HSVdg main variants with six new minor variants were found. They were randomly spread in the vineyard. The distributions of GYSVd1 and HSVdg main variants and their accompanying subvariants differed even in neighbouring plants. We conclude that these individual viroid variant profiles are the result of 20 years of independent evolution, i.e. mutation and selection, in each single plant. Four of the nine GYSVd1 main variants were mutated in the inverted repeats bordering the central conserved region. These base substitutions decreased the thermodynamic stability of a metastable structure called hairpin I.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-1-155
1996-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/1/JV0770010155.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-1-155&mimeType=html&fmt=ahah

References

  1. Candresse T., Diener T. O., Owens R. A. 1990; The role of the viroid central conserved region in cDNA infectivity. Virology 175:232–237
    [Google Scholar]
  2. Eigen M., McCaskill J., Schuster P. 1988; Molecular quasispecies. Journal of Physical Chemistry 92:6881–6891
    [Google Scholar]
  3. Flores R. 1995; Viroids. In Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses pp 495–497 Edited by Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. Wien & New York: Springer Verlag;
    [Google Scholar]
  4. Garcia-Arenal F., Pallas V., Flores R. 1987; The sequence of a viroid from grapevine closely related to severe isolates of citrus exocortis viroid. Nucleic Acids Research 15:4203–4210
    [Google Scholar]
  5. Keese P., Symons R. H. 1985; Domains in viroids: evidence of intermolecular RNA rearrangements and their contribution to viroid evolution. Proceedings of the National Academy of Sciences, USA 82:4582–4586
    [Google Scholar]
  6. Koltunow A. M., Rezaian M. A. 1988; Grapevine yellow speckle viroid: structural features of a new viroid group. Nucleic Acids Research 16:849–864
    [Google Scholar]
  7. Koltunow A. M., Rezaian M. A. 1989; Grapevine viroid IB, a new member of apple scar skin viroid group contains the left terminal region of tomato planta macho viroid. Virology 170:575–578
    [Google Scholar]
  8. Koltunow A. M., Krake L. R., Johnson S. D., Rezaian M. A. 1989; Two related viroids cause grapevine yellow speckle disease independently. Journal of General Virology 70:3411–3419
    [Google Scholar]
  9. Minafra A., Martelli G. P., Savino V. 1990; Viroids of grapevine in Italy. Vitis 29:173–182
    [Google Scholar]
  10. Myers T. W., Gelfand D. H. 1991; Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry 30:7661–7666
    [Google Scholar]
  11. Orita M., Iwahana H., Kanazawa H., Hayashi K., Sekiya T. 1989; Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proceedings of the National Academy of Sciences, USA 86:2766–2770
    [Google Scholar]
  12. Puchta H., Ramm K., Sanger H. L. 1988; Nucleotide sequence of a hop stunt viroid isolate from the German grapevine cultivar ‘Riesling’. Nucleic Acids Research 16:2730
    [Google Scholar]
  13. Rao V. B. 1994; Direct sequencing of polymerase chain reaction-amplified DNA. Analytical Biochemistry 216:1–14
    [Google Scholar]
  14. Rezaian M. A. 1990; Australian grapevine viroid - evidence for extensive recombination between viroids. Nucleic Acids Research 18:1813–1818
    [Google Scholar]
  15. Ridgen J. E., Rezaian M. A. 1993; Analysis of sequence variation in grapevine yellow speckle viroid 1 reveals two distinct alternative structures for the pathogenic domain. Virology 193:474–477
    [Google Scholar]
  16. Riesner D., Gross H. J. 1985; Viroids. Annual Reviews in Biochemistry 54:531–564
    [Google Scholar]
  17. Riesner D., Henco K., Rokohl U., Klotz G., Kleinschmidt A. K., Domdey H., Jank P., Gross H. J., Sanger H. L. 1979; Structure and structure formation of viroids. Journal of Molecular Biology 133:85–115
    [Google Scholar]
  18. Rodriguez M. J. B., Randles J. W. 1993; Coconut cadang-cadang viroid (CCCVd) mutants associated with severe disease vary in both the pathogenicity domain and the central conserved region. Nucleic Acids Research 21:2771
    [Google Scholar]
  19. Sano T., Ohshima K., Uyeda I., Shikata E., Meshi T., Okada Y. 1985; Nucleotide sequence of grapevine viroid: a grapevine isolate of hop stunt viroid. Proceedings of the Japan Academy 61:265–268
    [Google Scholar]
  20. Semancik J. S., Szychowski J. A. 1992; Relationships among the viroids derived from grapevines. Journal of General Virology 73:1465–1469
    [Google Scholar]
  21. Staub U., Polivka H., Gross H. J. 1995a; Two rapid microscale procedures for isolation of total RNA from leaves rich in polyphenols and polysaccharides: application for sensitive detection of grapevine viroids. Journal of Virological Methods 52:209–218
    [Google Scholar]
  22. Staub U., Polivka H., Herrmann J. V., Gross H. J. 1995b; Transmission of grapevine viroids is not likely to occur mechanically by regular pruning. Vitis 34:119–123
    [Google Scholar]
  23. Tinoco I. Jr, Borer P. N., Dengler B., Uhlenbeck O. C., Crothers D. M., Gralla J. 1973; Improved estimation of secondary structure in ribonucleic acids. Nature 246:40–41
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-1-155
Loading
/content/journal/jgv/10.1099/0022-1317-77-1-155
Loading

Data & Media loading...

Most cited Most Cited RSS feed