@article{mbs:/content/journal/jgv/10.1099/0022-1317-76-9-2387, author = "van Engelenburg, Frank A. C. and Kaashoek, Marianne J. and van Oirschot, Jan T. and Rijsewijk, Frans A. M.", title = "A glycoprotein E deletion mutant of bovine herpesvirus 1 infects the same limited number of tissues in calves as wild-type virus, but for a shorter period", journal= "Journal of General Virology", year = "1995", volume = "76", number = "9", pages = "2387-2392", doi = "https://doi.org/10.1099/0022-1317-76-9-2387", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-76-9-2387", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "To gain insight into the role of glycoprotein E of bovine herpesvirus 1 (BHV-1), we compared the distribution of wild-type (wt) BHV-1 with that of a gE deletion mutant (gE−) in calves after intranasal inoculation. The wt-infected calves had severe clinical signs, but the gE−-infected calves were virtually free of clinical signs. At 3, 4, 7, 8, 44, 45, 50 and 51 days post-infection (p.i.), one calf from each group was killed and tissues were collected for virus isolation and PCR analysis. At 3, 4, 7 and 8 days p.i., infectious virus could be isolated only from the nasopharyngeal mucosa, parotid gland and nearby lymphoid tissues for both the wt- and gE−-infected calves. At 3 and 4 days p.i., virus titres in these tissues were comparable in both the wt- and gE−-infected calves. However, the virus titres were significantly reduced at 7 and 8 days p.i. in the gE−-infected calves, but not in the wt-infected calves. Semi-quantitative PCR analysis revealed that for the entire infection period (3 to 51 days p.i.) significantly more BHV-1 DNA was detected in the trigeminal ganglia (TG) of the wt-infected calves than in those of the gE−-infected calves. We conclude that the gE− mutant infects the same limited number of tissues as wt BHV-1, but for a shorter period.", }