1887

Abstract

To gain insight into the role of glycoprotein E of bovine herpesvirus 1 (BHV-1), we compared the distribution of wild-type (wt) BHV-1 with that of a gE deletion mutant (gE) in calves after intranasal inoculation. The wt-infected calves had severe clinical signs, but the gE-infected calves were virtually free of clinical signs. At 3, 4, 7, 8, 44, 45, 50 and 51 days post-infection (p.i.), one calf from each group was killed and tissues were collected for virus isolation and PCR analysis. At 3, 4, 7 and 8 days p.i., infectious virus could be isolated only from the nasopharyngeal mucosa, parotid gland and nearby lymphoid tissues for both the wt- and gE-infected calves. At 3 and 4 days p.i., virus titres in these tissues were comparable in both the wt- and gE-infected calves. However, the virus titres were significantly reduced at 7 and 8 days p.i. in the gE-infected calves, but not in the wt-infected calves. Semi-quantitative PCR analysis revealed that for the entire infection period (3 to 51 days p.i.) significantly more BHV-1 DNA was detected in the trigeminal ganglia (TG) of the wt-infected calves than in those of the gE-infected calves. We conclude that the gE mutant infects the same limited number of tissues as wt BHV-1, but for a shorter period.

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1995-09-01
2022-05-19
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References

  1. Bagust J. T., Clark L. 1972; Pathogenesis of meningoencephalitis produced in calves by infectious bovine rhinothracheitis herpesvirus. Journal of Comparative Pathology 82:375–383
    [Google Scholar]
  2. Brenner J., Ungar-Waron H., Elad D., Abraham A. 1989; Clinical and serological follow up of young calves experimentally infected with virulent bovine herpes virus (BHV-1). Israel Journal of Veterinary Medicin 45:21–25
    [Google Scholar]
  3. Card J. P., Whealy M. E., Robbins A. K., Moore R. Y., Enquist L.W. 1991; Two-herpesvirus strains are transported differentially in the rodent visual system. Neuron 6:957–969
    [Google Scholar]
  4. Dingwell K. S., Brunetti C. R., Hendricks R. L., Tang Q., Tang M., Rainbow A. J., Johnson D. C. 1994; Herpes simplex virus glycoproteins E and I facilitate cell-to-cell spread in vivo and across junctions of cultured cells. Journal of Virology 68:834–845
    [Google Scholar]
  5. Gibbs E. P. J., Rweyemamu M. M. 1977; Bovine herpesviruses. I. Bovine herpesvirus 1. Veterinary Bulletin 47:317–343
    [Google Scholar]
  6. Gustafson D. P. 1986; Pseudorabies. In Diseases of Swine pp 274–289 Edited by Leman A. D., Straw B., Glock R. D., Mengeling W. L., Penny R. N. C., Schull E. S. Ames: Iowa State University Press;
    [Google Scholar]
  7. Kaashoek M. J., Moerman A., Madic J., Rijsewijk F. A. M., Quak J., Gielkens A. L. J., Van Oirschot J. T. 1994; A conventionally attenuated glycoprotein E negative strain of bovine herpesvirus type 1 is an efficacious and safe vaccine. Vaccine 12:439–144
    [Google Scholar]
  8. Kaye S., Loveday C., Tedder R. S. 1991; Storage and preservation of whole blood samples for use in detection of human immunodeficiency virus type-1 by the polymerase chain reaction. Journal of Virological Methods 35:217–226
    [Google Scholar]
  9. Kimman T. G., De Wind N., Oei-Lie N., Pol J. M. A., Berns A. J. M., Gielkens A. L. J. 1992a; Contribution of single genes within the unique short region of Aujeszky’s disease virus (suid herpesvirus type 1) to virulence, pathogenesis and immunogenicity. Journal of General Virology 73:243–251
    [Google Scholar]
  10. Kimman T. G., Pol J. M. A., De Wind N., Oei-Lie N., Berns A. J. M., Gielkens A. L. J. 1992b; Role of different genes in the virulence and pathogenesis of Aujeszky’s disease virus. Veterinary Microbiology 33:45–52
    [Google Scholar]
  11. Kúdelová M., Kostal M., Cervenáková L., Raicáni J., Kaerner H. C. 1991; Pathogenicity and latency competence for rabbits of the herpes simplex virus type 1 ANGpath gC and gE defective mutants. Acta Virologica 35:438–449
    [Google Scholar]
  12. Rajcáni J., Herget U., Kaerner H. C. 1990a; Spread of herpes simplex virus (HSV) strains SC 16, ANG, ANGpath and its GlyC minus and GlyE minus mutants in DBA-2 mice. Acta Virologica 34:305–320
    [Google Scholar]
  13. Rajcáni J., Herget U., Kostál M., Kaerner H. C. 1990b; Latency competence of herpes simplex virus strains ANG, ANGpath and its gC and gE minus mutants. Acta Virologica 34:477–486
    [Google Scholar]
  14. Roizman B., Sears A. E. 1987; An inquiry into the mechanism of herpes simplex virus latency. Annual Review of Microbiology 41:543–571
    [Google Scholar]
  15. Spear P. G. 1993; Entry of alphaherpesviruses into cells. Seminars in Virology 4:167–180
    [Google Scholar]
  16. Van Engelenburg F. A. C., Maes R. K., Van Oirschot J. T., Rijsewijk F. A. M. 1993; Development of a rapid and sensitive polymerase chain reaction assay for the detection of bovine herpesvirus type 1 in bovine semen. Journal of Clinical Microbiology 31:3129–3135
    [Google Scholar]
  17. Van Engelenburg F. A. C., Kaashoek M. J., Rijsewuk F. A. M., Van den Burg L., Moerman A., Gielkens A. L. J., Van Oirschot J. T. 1994; A glycoprotein E deletion mutant of bovine herpesvirus 1 is avirulent in calves. Journal of General Virology 75:2311–2318
    [Google Scholar]
  18. Zsak L., Zuckermann F., Sugg N., Ben-Porat T. 1992; Glycoprotein gl of pseudorabies virus promotes cell fusion and virus spread via direct cell-to-cell transmission. Journal of Virology 66:2316–2325
    [Google Scholar]
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