1887

Abstract

Recently, data demonstrating that CD4 is an essential component of the receptor for human herpesvirus 7 (HHV-7) as well as for human immunodeficiency virus have been accumulating. Since gangliosides and phorbol esters are known to induce selective down-modulation of cell surface CD4 expression, it might be expected that treatment with these agents would interfere with HHV-7 infection of CD4 T cells. The present study, undertaken to verify this possibility, demonstrated that addition of monosialoganglioside-GM1 or 12--tetradecanoylphorbol 13-acetate effectively induced disappearance of CD4 from the cell surface and also reduced HHV-7 infectivity, as judged by the CPE on virus-infected cells and studies of indirect immunofluorescence, TCID and semi-quantitative PCR of the HHV-7 genome. Taken together with previous studies, the present data strongly suggest that the CD4 molecule is a critical component of the receptor for HHV-7.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-9-2381
1995-09-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/9/JV0760092381.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-9-2381&mimeType=html&fmt=ahah

References

  1. Berneman Z. N., Ablashi D. V., Li G., Eger-Fletcher M., Reitz M. S. Jr, Hung C.-L., Brus I., Komaroff A. L., Gallo R. C. 1992; Human herpesvirus 7 is a T-lymphotropic virus and is related to, but significantly different from, human herpesvirus 6 and human cytomegalovirus. Proceedings of the National Academy of Sciences, USA 89:10552–10556
    [Google Scholar]
  2. Chieco-Bianchi L., Calabro M. L., Panozzo M., Rossi A. D., Amadori A., Callegaro L., Siccardi A. 1989; CD4 modulation and inhibition of HIV-1 infectivity induced by mono-sialoganglioside GM1 in vitro . AIDS 3:501–507
    [Google Scholar]
  3. Frenkel N., Schirmer E. C., Wyatt L. S., Katsafanas G., Roffman E., Danovich R. M., June C. H. 1990; Isolation of a new herpesvirus from human CD4+ T cells. Proceedings of the National Academy of Sciences, USA 87:748–752
    [Google Scholar]
  4. Furukawa M., Yasukawa M., Yakushijin Y., Fujita S. 1994; Distinct effects of human herpesvirus 6 and human herpesvirus 7 on surface molecule expression and function of CD4+ T cells. Journal of Immunology 152:5768–5775
    [Google Scholar]
  5. Geleziunas R., Bour S., Wainberg M. A. 1994; Cell surface down-modulation of CD4 after infection by HIV-1. FASEB Journal 8:593–600
    [Google Scholar]
  6. Hoxie J. A., Alpers J. D., Rackowski J. L., Huebner K., Haggarty B. S., Cedarbaum A. J., Reed J. C. 1986a; Alterations in T4 (CD4) protein and mRNA synthesis in cells infected with HIV. Science 234:1123–1127
    [Google Scholar]
  7. Hoxie J. A., Matthews D. M., Callahan K. J., Cassel D. L., Cooper R. A. 1986b; Transient modulation and internalization of T4 antigen induced by phorbol esters. Journal of Immunology 137:1194–1201
    [Google Scholar]
  8. Kawaguchi T., Nakakuma H., Kagimoto T., Shirono K., Horikawa K., Hidaka M., Iwamori M., Nagai Y., Takatsuki K. 1989; Characteristic mode of action of gangliosides in selective modulation of CD4 on human T lymphocytes. Biochemical and Biophysical Research Communications 158:1050–1059
    [Google Scholar]
  9. Kawamura I., Koga Y., Oh-Hori N., Onodera K., Kimura G., Nomoto K. 1989; Depletion of the surface CD4 molecule by the envelope protein of human immunodeficiency virus expressed in a human CD4+ monocytoid cell line. Journal of Virology 63:3748–3754
    [Google Scholar]
  10. Lusso P., Secchiero P., Crowley R. W., Garzino-Demo A., Berneman Z. N., Gallo R. C. 1994; CD4 is a critical component of the receptor for human herpesvirus 7: interference with human immunodeficiency virus. Proceedings of the National Academy of Sciences, USA 91:3872–3876
    [Google Scholar]
  11. Neudorf S., Jones M., Parker S., Papes R., Lattier D. 1991; Phorbol esters down-regulate transcription and translation of the CD4 gene. Journal of Immunology 146:2836–2840
    [Google Scholar]
  12. Offner H., Thieme T., Vandenbark A. A. 1987; Gangliosides induce selective modulation of CD4 from helper T lymphocytes. Journal of Immunology 139:3295–3305
    [Google Scholar]
  13. Reed L. J., Muench H. 1938; A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  14. Salmon P., Olivier R., Riviere Y., Brisson E., Gluckman J.-C., Kieny M.-P., Montagnier L., Klatzmann D. 1988; Loss of CD4 membrane expression and CD4 mRNA during acute human immunodeficiency virus replication. Journal of Experimental Medicine 168:1953–1969
    [Google Scholar]
  15. Stevenson M., Zhang X., Volsky D. J. 1987; Downregulation of cell surface molecules during noncytopathic infection of T cells with human immunodeficiency virus. Journal of Virology 61:3741–3748
    [Google Scholar]
  16. Yakushijin Y., Yasukawa M., Kobayashi Y. 1991; T-cell immune response to human herpesvirus-6 in healthy adults. Microbiology and Immunology 35:655–660
    [Google Scholar]
  17. Yalcin S., Mukai T., Kondo K., Ami Y., Okawa T., Kojima A., Kurata T., Yamanishi K. 1992; Experimental infection of cynomolgus and African green monkeys with human herpesvirus 6. Journal of General Virology 731673–1677
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-9-2381
Loading
/content/journal/jgv/10.1099/0022-1317-76-9-2381
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error