1887

Abstract

All alpha herpesviruses of known DNA sequence have been found to encode a protein with similarities to immediate early protein Vmw110 (ICP0) of herpes simplex virus type 1 (HSV-1). The conserved portion of this family of proteins is a characteristic zinc binding module, known as a RING finger or CHC domain. Examples of RING finger domains occur in many other proteins of diverse evolutionary origin and function. Recently, the solution structure of the equine herpesvirus 1 (EHV-1) RING finger protein, encoded by gene 63, has been solved. To investigate whether this structure could be considered to be a paradigm of herpesvirus RING domains, we have constructed a recombinant HSV-1 which expresses the EHV-1 gene 63 protein (EHVg63) in place of Vmw110. Comparison of the growth properties of the recombinant with those of wild-type and Vmw110-defective viruses indicates that EHVg63 is able to fulfil partially, but not completely, the roles of Vmw110 during virus growth in tissue culture.

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1995-09-01
2024-12-06
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References

  1. Barlow P. N., Luisi B., Milner A., Elliott M., Everett R. 1994; Structure of the C3HC4 domain by 1H nuclear magnetic resonance spectroscopy – a new structural class of zinc finger. Journal of Molecular Biology 237:201–211
    [Google Scholar]
  2. Desai P., Ramakrishnan R., Lin Z. W., Osak B., Glorioso J. C., Levine M. 1993; The RR1 gene of herpes simplex virus type 1 is uniquely transactivated by ICPO during infection. Journal of Virology 67:6125–6135
    [Google Scholar]
  3. Everett R. D. 1986; A detailed mutational analysis of Vmw110, a trans–acting transcriptional activator encoded by herpes simplex virus type 1. EMBO Journal 6:2069–2076
    [Google Scholar]
  4. Everett R. D. 1988; Analysis of the functional domains of herpes simplex virus type 1 immediate-early polypeptide Vmw110. Journal of Molecular Biology 202:87–96
    [Google Scholar]
  5. Everett R. D. 1989; Construction and characterization of herpes simplex virus type 1 mutants with defined lesions in immediate-early gene 1. Journal of General Virology 70:1185–1202
    [Google Scholar]
  6. Everett R. D., Maul G. G. 1994; HSV-1 IE protein Vmw110 causes redistribution of PML. EMBO Journal 13:5062–5069
    [Google Scholar]
  7. Everett R. D., Preston C. M., Stow N. D. 1991; Functional and genetic analysis of the role of Vmw110 in herpes simplex virus replication. In Herpes Virus Transcription and its Regulation Edited by Wagner E. K. Boca Raton: CRC Press;
    [Google Scholar]
  8. Everett R. D., Maul G. G., Orr A., Elliott M. 1995; The cellular RING finger protein PML is not a functional counterpart of the herpes simplex virus type 1 RING finger protein Vmw110. Journal of General Virology 76:791–798
    [Google Scholar]
  9. Freemont P. 1993; The RING finger. A novel protein sequence related to the zinc finger. Annals of the New York Academy of Sciences 684:174–192
    [Google Scholar]
  10. Freemont P. S., Hanson I. M., Trowsdale J. 1991; A novel cysteine-rich motif. Cell 64:483–184
    [Google Scholar]
  11. Harris R. A., Everett R. D., Zhu X. X., Silverstein S., Preston C. M. 1989; Herpes simplex virus type 1 immediate-early protein Vmw110 reactivates latent herpes simplex virus type 2 in an in vitro latency system. Journal of Virology 63:3513–3515
    [Google Scholar]
  12. McGeoch D. J., Barnett B. C., MacLean C. A. 1993; Emerging functions of alphaherpesvirus genes. Seminars in Virology 4:125–134
    [Google Scholar]
  13. Maul G. G., Everett R. D. 1994; The nuclear location of PML, a cellular member of the C3HC4 zinc-binding domain protein family, is rearranged during herpes simplex virus infection by the C3HC4 viral protein ICPO. Journal of General Virology 75:1223–1233
    [Google Scholar]
  14. Maul G. G., Guldner H. H., Spivack J. G. 1993; Modification of discrete nuclear domains induced by herpes simplex virus type 1 immediate-early gene 1 product ICPO. Journal of General Virology 74:2679–2690
    [Google Scholar]
  15. Meredith M., Orr A., Everett R. D. 1994; Herpes simplex virus type 1 immediate-early protein Vmw110 binds strongly and specifically to a 135 kDa cellular protein. Virology 200:457–169
    [Google Scholar]
  16. Meredith M., Orr A., Elliott M., Everett R. D. 1995; Separation of the sequence requirements for HSV-1 Vmw110 multimerisation and interaction with a 135 kDa cellular protein. Virology 209:174–187
    [Google Scholar]
  17. Moriuchi H., Moriuchi M., Smith H. A., Straus S. E., Cohen J. I. 1992; Varicella zoster virus open reading frame 61 protein is functionally homologous to herpes simplex virus type 1 ICPO. Journal of Virology 66:7303–7308
    [Google Scholar]
  18. Moriuchi H., Moriuchi M., Straus S. E., Cohen J. I. 1993; Varicella zoster virus (VZV) open reading frame 61 protein transactivates VZV gene promoters and enhances the infectivity of VZV DNA. Journal of Virology 67:4290–4295
    [Google Scholar]
  19. Moriuchi H., Moriuchi M., Cohen J. I. 1994; The RING finger domain of the varicella zoster virus open reading frame 61 protein is required for its transregulatory functions. Virology 205:238–246
    [Google Scholar]
  20. Nagpal S., Ostrove J. M. 1991; Characterization of a potent varicella zoster virus-encoded trans-repressor. Journal of Virology 65:5289–5296
    [Google Scholar]
  21. Roizman B., Sears A. E. 1990; Herpes simplex viruses and their replication. In Virology 2nd edn vol 2 pp 1795–1842 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  22. Stow N. D., Stow E. C. 1986; Isolation and characterization of a herpes simplex virus type 1 mutant containing a deletion within the gene encoding the immediate-early polypeptide Vmw110. Journal of General Virology 67:2571–2585
    [Google Scholar]
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