1887

Abstract

Banzi is a mosquito borne flavivirus which belongs to the Uganda S serocomplex. No nucleotide sequence data have previously been reported from any virus of this serocomplex. We have determined the nucleotide sequence of the NS5 gene from Banzi virus and the predicted amino acid sequence was elucidated. Previously identified conserved RNA polymerase, methyltransferase and flavivirus NS5 amino acid motifs were present in the Banzi virus NS5 protein. These data add to the evidence for the functional importance of the regions. The encoded amino acid sequence was compared with the predicted amino acid sequence of other flavivirus NS5 proteins. Analysis of these sequences suggested that Banzi virus is most closely related to the mosquito-borne flaviviruses and, in particular, yellow fever virus. This pattern of similarity is in accordance with the previously suggested serological classification of flaviviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-9-2317
1995-09-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/9/JV0760092317.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-9-2317&mimeType=html&fmt=ahah

References

  1. Bartholomeusz A. I., Wright P. J. 1993; Synthesis of dengue virus RNA in vitro: initiation and the involvement of proteins NS3 and NS5. Archives of Virology 128:111–121
    [Google Scholar]
  2. Calisher C. H., Karabatsos N., Dalrymple J., Shope R. E., Porterfield J. S., Westaway E. G., Brandt W. E. 1989; Antigenic relationships between flaviviruses as determined by crossneutralization tests with polyclonal antisera. Journal of General Virology 70:37–13
    [Google Scholar]
  3. Edward Z., Takegami T. 1993; Localization and functions of Japanese encephalitis virus nonstructural proteins NS3 and NS5 for viral RNA synthesis in the infected cells. Microbiology and Immunology 37:239–243
    [Google Scholar]
  4. Fulop L., Barrett A. D. T., Phillpotts R., Martin K., Leslie D., Titball R. W. 1993; Rapid identification of flaviviruses based on conserved NS5 gene sequences. Journal of Virological Methods 44:179–188
    [Google Scholar]
  5. Heinz F. X., Mandl C. W., Guirakhoo F., Holzmann H., Tuma W., Kunz C. 1990; The envelope protein E of tick-borne encephalitis virus and other flaviviruses: structure, functions and evolutionary relationships. Archives of Virology Supplement 1:125–135
    [Google Scholar]
  6. Ishihama A., Barbier P. 1994; Molecular anatomy of viral RNA-directed RNA polymerases. Archives of Virology 134:235–258
    [Google Scholar]
  7. Kamer G., Argos P. 1984; Primary structure comparison of RNA-dependent polymerases from plant, animal and bacterial viruses. Nucleic Acid Research 12:7269–7282
    [Google Scholar]
  8. Kokernot R. H., Casaca V. M. R., Weinbren M. P., McIntosh B. M. 1965; Survey for antibodies against arthropod-borne viruses in the sera of indigenous residents of Angola. Transactions of the Royal Society of Tropical Medicine and Hygiene 59:563–570
    [Google Scholar]
  9. Koonin E. V. 1991; The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. Journal of General Virology 72:2197–2206
    [Google Scholar]
  10. Koonin E. V. 1993; Computer-assisted identification of a putative methyltransferase domain in NS5 protein of flaviviruses and lambda 2 protein of reovirus. Journal General Virology 74:733–740
    [Google Scholar]
  11. Lin C., Chambers T. J., Rice C. M. 1993; Mutagenesis of conserved residues at the yellow fever virus 3/4A and 4B/5 dibasic cleavage sites: effects on cleavage efficiency and polyprotein processing. Virology 192:596–604
    [Google Scholar]
  12. McIntosh B. M. 1961; Susceptibility of some African wild rodents to infection with various arthropod-borne viruses. Transactions of the Royal Society of Tropical Medicine and Hygiene 55:63–68
    [Google Scholar]
  13. McIntosh B. M., Dos Santos I. S. L., Meenehan G. M. 1976a; Culex (Eumelanomyia) Rubinotus. Theobald as vector of Banzi, Germiston and Witwatersrand viruses. Journal of Medical Entomology 12:645–646
    [Google Scholar]
  14. McIntosh B. M., Jupp P. G., Dos Santos I. S. L., Meenehan G. M. 1976b; Culex(Eumelanomyia) Rubinotus Theobald as vector of Banzi, Germiston and Witwatersrand viruses 1. Isolation of virus from wild populations of C. rubinotus. Journal of Medical Entomology 12:637–640
    [Google Scholar]
  15. Marin M. S., Zanotto P. M. DE A., Gritsun T. S., Gould E. A. 1994; Phylogeny of TYU, SRE and CFA virus: Different evolutionary rates in the genus Flavivirus . Virology 206:1133–1139
    [Google Scholar]
  16. Metslaar D., Henderson B. E., Kirya G. B., Tukei P. M. 1974; Isolation of arboviruses in Kenya, 1966-1971. Transactions of the Royal Society of Tropical Medicine and Hygiene 68:114–123
    [Google Scholar]
  17. Pierre V., Drouet M. T., Deubel V. 1994; Identification of mosquito-borne flavivirus sequences using universal primers and reverse transcription/polymerase chain reaction. Research in Virology 145:93–104
    [Google Scholar]
  18. Pletnev A. G., Yamshchikov V. F., Blinov V. M. 1990; Nucleotide sequence of the genome and complete amino acid sequence of the polyprotein of tick-borne encephalitis virus. Virology 174:250–263
    [Google Scholar]
  19. Poch O., Sauvaget I., Delarue M., Tordo N. 1989; Identification of four conserved motifs among the RNA-dependent polymerase encoding elements. EMBO Journal 8:3867–3874
    [Google Scholar]
  20. Smithburn K. C., Paterson H. E., Heymann C. S., Winter P. A. D. 1959; An agent related to Uganda S. virus from man and mosquitoes in South Africa. South African Medical Journal 46:959–962
    [Google Scholar]
  21. Venugopal K., Gritsun T., Gould E. A. 1994; Analysis of the structural protein gene sequence shows Kyasanur Forest disease virus as a distinct member in the tick-borne encephalitis virus serocomplex. Journal of General Virology 75:227–232
    [Google Scholar]
  22. Williams M. C., Woodall J. P. 1964; An epidemic of an illness resembling Dengue in the Morogoro district of Tanganyika. East African Medical Journal 41:271–275
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-9-2317
Loading
/content/journal/jgv/10.1099/0022-1317-76-9-2317
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error