1887

Abstract

The complete nucleotide sequence of citrus tatter leaf capillovirus (CTLV lily strain) was determined. It is 6496 nucleotides long, excluding the 3′-terminal poly(A) tract, and contains two putative overlapping open reading frames (ORFs). ORF1 (positions 37-6354) encodes a potential polyprotein of molecular mass 242 kDa. ORF2 (positions 4788-5750) codes for a 36 kDa protein. The 242 kDa polypeptide contains several non-structural protein domains (i.e. methyltransferase, NTP-binding helicase, papain-like proteinase and polymerase) and, at its C terminus, the putative coat protein. The N-terminal region of the 36 kDa protein displays sequence similarity to the cell-to-cell movement proteins of the ‘30 K superfamily’. Such a genome structure is conserved between CTLV and apple stem grooving capillovirus. Capped transcripts from a plasmid containing the complete sequence of CTLV, with a T7 RNA promoter, successfully infected plants and caused symptoms characteristic of CTLV. Uncapped transcripts were non-infectious.

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1995-09-01
2022-01-16
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References

  1. Ahmed N. A., Christie S. R., Zettler F. W. 1983; Identification and partial characterization of a closterovirus infecting Nandina domestica. Phytopathology 73:470–475
    [Google Scholar]
  2. Brunt A. A. 1978; The occurence, hosts and properties of lilac chlorotic leaf spot virus, a newly recognized virus from Syringa vulgaris. Annals of Applied Biology 88:383–391
    [Google Scholar]
  3. Coffin R. S., Coutts R. H. A. 1993; The closteroviruses, capilloviruses and other similar viruses: a short review. Journal of General Virology 74:1475–1483
    [Google Scholar]
  4. Deborde D. S., Naeve C. W., Herlocher M. L., Maassab H. F. 1986; Resolution of a common RNA sequencing ambiguity by terminal deoxynucleotidyl transferase. Analytical Biochemistry 157:275–282
    [Google Scholar]
  5. German S., Candresse T., Lanneau M., Huet J. C., Pernollet J. C., Dunez J. 1990; Nucleotide sequence and genomic organization of apple chlorotic leaf spot closterovirus. Virology 179:104–112
    [Google Scholar]
  6. Gorbalenya A. E., Koonin E. V., Donchenko A. P., Blinov V. M. 1988; A conserved NTP-motif in putative helicases. Nature 333:22
    [Google Scholar]
  7. Koonin E. V. 1991; The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. Journal of General Virology 72:2197–2206
    [Google Scholar]
  8. Lister R. M. 1970; Apple stem grooving virus. CMI/AAB Descriptions of Plant Viruses no. 31
    [Google Scholar]
  9. Lütcke H. A., Chow K. C., Mickel F. S., Moss K. A., Kern H. F., Sheele G. A. 1987; Selection of AUG initiation codons differs in plants and animals. EM BO Journal 6:43–18
    [Google Scholar]
  10. Martelli G. P., Candresse T., Namba S. 1994; Trichovirus, a new genus of plant viruses. Archives of Virology 134:451–455
    [Google Scholar]
  11. Mushegian A. R., Koonin E. V. 1993; Cell-to-cell movement of plant viruses. Archives of Virology 133:239–257
    [Google Scholar]
  12. Nishio T., Kawai A., Takahashi T., Namba S., Yamashita S. 1989; Purification and properties of citrus tatter leaf virus. Annals of the Phytopathological Society of Japan 55:254–258
    [Google Scholar]
  13. Ochi M., Kashiwazaki S., Hiratsuka K., Namba S., Tsuchizaki T. 1992; Nucleotide sequence of the 3′-terminal region of potato virus T RNA. Annals of the Phytopathological Society of Japan 58:416–425
    [Google Scholar]
  14. Ohira K., Ito T., Kawai A., Namba S., Kusumi T., Tsuchizaki T. 1994; Nucleotide sequence of the 3′-terminal region of citrus tatter leaf virus lily strain. Virus Genes 8:165–167
    [Google Scholar]
  15. Rozanov M. N., Koonin E. V., Gorbalenya E. 1992; Conservation of the putative methyltransferase domain: a hallmark of the ‘Sindbis-like’ supergroup of positive-strand RNA viruses. Journal of General Virology 73:2129–2134
    [Google Scholar]
  16. Rozanov M. N., Drugeon G., Haenni A. L. 1995; Papain-like proteinase of turnip yellow mosaic virus: a prototype of a new viral proteinase group. Archives of Virology 140:273–288
    [Google Scholar]
  17. Salazar L. F., Harrison B. D. 1978; Host range, purification and properties of potato virus T. Annals of Applied Biology 89:223–235
    [Google Scholar]
  18. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  19. Yoshikawa N., Sasaki E., Kato M., Takahashi T. 1992; The nucleotide sequence of apple stem grooving capillovirus genome. Virology 191:98–105
    [Google Scholar]
  20. Yoshikawa N., Imaizumi M., Takahashi T., Inouye N. 1993; Striking similarities between the nucleotide sequence and genome organization of citrus tatter leaf and apple stem grooving capilloviruses. Journal of General Virology 742743–2747
    [Google Scholar]
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