1887

Abstract

Tomato ringspot nepovirus (TomRSV) produces a 45 kDa movement protein and a 58 kDa coat protein in infected plants. Accumulation of the movement protein in relation to that of the coat protein was studied in infected protoplasts using a monoclonal antibody against the movement protein and polyclonal antibodies against the coat protein. Unlike most other viral movement proteins, the TomRSV movement protein was present at late stages of infection. Pulse-chase labelling experiments revealed that the release of the movement protein from the precursor polyprotein was coordinated with that of the coat protein. However, the movement protein was less stable than the coat protein in the extractable fraction of the protoplasts. The expression pattern of the TomRSV movement protein is discussed in the light of the proposed mechanism of cell-to-cell movement of virus-like particles through tubular structures composed of the movement protein.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-9-2299
1995-09-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/9/JV0760092299.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-9-2299&mimeType=html&fmt=ahah

References

  1. Blum H., Gross H. J., Beier H. 1989; The expression of TMV-speciflc 30 kDa protein in tobacco protoplasts is strongly and selectively enhanced by actinomycin. Virology 169:51–61
    [Google Scholar]
  2. Demangeat G., Hemmer O., Reinbolt J., Mayo M. A., Fritsch C. 1992; Virus-specific proteins in cells infected with tomato black ring nepovirus: evidence for proteolytic processing in vivo . Journal of General Virology 73:1609–1614
    [Google Scholar]
  3. Hans F., Sanfaҫon H. 1995; Tomato ringspot nepovirus protease: characterization and cleavage site specificity. Journal of General Virology 76:917–927
    [Google Scholar]
  4. Hibrand L., Le Gall O., Candresse T., Dunez J. 1992; Immunodetection of the proteins encoded by grapevine chrome mosaic nepovirus RNA2. Journal of General Virology 73:2093–2098
    [Google Scholar]
  5. Linstead P. J., Hills G. J., Plaskitt K. A., Wilson I. G., Harker C. L., Maule A. J. 1988; The subcellular localization of the gene 1 product of cauliflower mosaic virus is consistent with a function associated with virus spread. Journal of General Virology 69:1809–1818
    [Google Scholar]
  6. Maule A. J. 1991; Virus movement in infected plants. Critical Reviews in Plant Sciences 9:457–473
    [Google Scholar]
  7. Perbal M.-C., Thomas C. L., Maule A. J. 1993; Cauliflower mosaic virus gene I product (PI) forms tubular structures which extend from the surface of infected protoplasts. Virology 195:281–285
    [Google Scholar]
  8. Rezelman G., Van Kammen A., Wellink J. 1989; Expression of cowpea mosaic virus M RNA in cowpea protoplasts. Journal of General Virology 70:3043–3050
    [Google Scholar]
  9. Ritzenthaler C., Pinck M., Pinck L. 1995; Grapevine fanleaf nepovirus P38 putative movement protein is not transiently expressed and is a stable final maturation product in vivo . Journal of General Virology 76:907–915
    [Google Scholar]
  10. Rott M. E., Tremaine J. H., Rochon D. M. 1991; Nucleotide sequence of tomato ringspot virus RNA2. Journal of General Virology 72:1505–1515
    [Google Scholar]
  11. Rott M. E., Gilchrist A., Lee L., Rochon D. M. 1995; Nucleotide sequence of tomato ringspot virus RNA1. Journal of General Virology 76:465–473
    [Google Scholar]
  12. Shanks M., Tomenius K., Clapham D., Huskison N., Barker P., Wilson I. G., Maule A. J., Lomonossoff G. P. 1989; Identification and subcellular localisation of a putative cell-to-cell transport protein from red clover mottle virus. Virology 173:400
    [Google Scholar]
  13. Van Lent J., Wellink J., Goldbach R. 1990; Evidence for the involvement of the 58K and 48K proteins in the intercellular movement of cowpea mosaic virus. Journal of General Virology 71:219–223
    [Google Scholar]
  14. Van Lent J., Storms M., Van der Meer F., Wellink J., Goldbach R. 1991; Tubular structures involved in movement of cowpea mosaic virus are also formed in infected cowpea protoplasts. Journal of General Virology 72:2615–2623
    [Google Scholar]
  15. Watanabe Y., Emori Y., Ooshika I., Meshi T., Ohno T., Okada Y. 1984; Synthesis of TMV-specific RNAs and proteins at the early stage of infection in tobacco protoplasts: transient expression of the 30K protein and its mRNA. Virology 133:18–24
    [Google Scholar]
  16. Wellink J., Jaegle M., Prinz H., Van Kammen A., Goldbach R. 1987; Expression of the middle component RNA of cowpea mosaic virus in vivo . Journal of General Virology 68:2577–2585
    [Google Scholar]
  17. Wellink J., Van Lent J. W. M., Verver J., Sijen T., Goldbach R. W., Van Kammen A. 1993; The cowpea mosaic virus M RNA-encoded 48-kilodalton protein is responsible for induction of tubular structures in protoplasts. Journal of Virology 67:3660–3664
    [Google Scholar]
  18. Wieczorek A., SantagoN H. 1993; Characterization and subcellular localization of tomato ringspot nepovirus putative movement protein. Virology 194:734–742
    [Google Scholar]
  19. Wieczorek A., Sanfaqon H. 1995; An improved method for the generation and transfection of protoplasts from Cucumis sativus cotyledons. Plant Cell Reports (in press)
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-9-2299
Loading
/content/journal/jgv/10.1099/0022-1317-76-9-2299
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error