A citrus exocortis viroid variant from broad bean ( L.): infectivity and pathogenesis Free

Abstract

A viroid present in very low titres was isolated from symptomless field broad bean plants. It was identified as a variant of citrus exocortis viroid in the T, V and C domains. Infection of several hosts resulted in a change in the composition of the viroid population. Serial passage through tomato and back to the host of origin, broad bean, resulted in major changes in replication efficiency, host range and pathogenicity. The unique nucleotide sequence differences identified in the original broad bean variant were not conserved after passage through alternative hosts. The effects of these sequence variations on viroid secondary structure result in nonpathogenic viroid variants which can remain unnoticed in certain plant species but may act as reservoirs of viroid disease.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-9-2271
1995-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/9/JV0760092271.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-9-2271&mimeType=html&fmt=ahah

References

  1. Diener T. O. 1987; Biological Properties. In The Viroids pp 9–35 Edited by Diener T. O. New York: Plenum Press;
    [Google Scholar]
  2. Diener T. O., Raymer W. B. 1969; Potato spindle tuber virus: aplant virus with properties of a free nucleic acid. Virology 37:351–366
    [Google Scholar]
  3. Fagoaga C., Pina J. A., Duran-Vila N. 1994; Occurrence of small RNAs in severely diseased vegetable crops. Plant Disease 78:749–753
    [Google Scholar]
  4. Fernow K. H. 1967; Tomato as a test plant for detecting mild strains of potato spindle tnber virus. Phytopathology 57:1344–1352
    [Google Scholar]
  5. Galindo J., Smith D. R., Diener T. O. 1982; Etiology of planta macho, a viroid disease of tomato. Phytopathology 72:49–54
    [Google Scholar]
  6. García-Arenal F., Pallas V., Flores R. 1987; The sequence of a viroid from grapevine closely related to severe isolates of citrus exocortis viroid. Nucleic Acids Research 15:4203–4210
    [Google Scholar]
  7. Gross FI. J., Krupp G., Domdey H., Raba M., Jank P., Lossow C., Alberty FF., Ramm K., Sänger H. L. 1982; Nucleotide sequence and secondary structure of citrus exocortis and chrysanthemum stunt viroid. European Journal of Biochemistry 121:249–257
    [Google Scholar]
  8. FIammond R. W., Owens R. A. 1987; Mutational analysis of potato spindle tuber viroid reveals complex relationships between structure and infectivity. Proceedings of the National Academy of Sciences, USA 84:3967–3971
    [Google Scholar]
  9. Igloi G. L. 1983; Silver stain for the detection of nanogram amounts of tRNA following two-dimensional electrophoresis. Analytical Biochemistry 134:184–188
    [Google Scholar]
  10. Ishikawa M., Meshi T., Okada Y., Sano T., Shikata E. 1985; In vitro mutagenesis of infectious cDNA clone. Journal of Biochemistry 98:1615–1620
    [Google Scholar]
  11. Keese P., Symons R. H. 1985; Domains in viroids: evidence of intermolecular rearragements and their contribution to viroid evolution. Proceedings of the National Academy of Sciences, USA 82:4582–4586
    [Google Scholar]
  12. Maniatis T., Fritsch E. F., Sambrook J. 1989 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Mishra M. D., Hammond R. W., Owens R. A., Smith D. R., Diener T. O. 1991; Indian bunchy top disease of tomato plants is caused by a distinct strain of citrus exocortis viroid. Journal of General Virology 72:1781–1785
    [Google Scholar]
  14. Owens R. A., Hammond R. W., Gardner R. C., Kiefer M. C., Thompson S. M., Cress D. E. 1986; Site-specific mutagenesis of potato spindle tuber viroid cDNA: alterations within premelting region 2 that abolish the infectivity. Plant Molecular Biology 6:179–192
    [Google Scholar]
  15. Puchta H., Sänger H. L. 1988; An improved procedure for the rapid one-step-cloning of full-length viroid cDNA. Archives of Virology 101:137–140
    [Google Scholar]
  16. Rivera-Bustamante R. F., Gin R., Semancik J. S. 1986; Enhanced resolution of circular and linear molecular forms of viroid and viroid-like RNA by electrophoresis in a discontinuous-pH system. Anaytical Biochemistry 156:91–95
    [Google Scholar]
  17. Schnolzer M., Hass B., Ramm K., Hofmann H., Sänger H. L. 1985; Correlation between structure and pathogenicity of potato spindle tuber viroid (PSTVd). EMBO Journal 4:2181–2190
    [Google Scholar]
  18. Semancik J. S. 1986; Separation of viroid-RNA by cellulose chromatography indicating conformational variations. Virology 155:39–45
    [Google Scholar]
  19. Semancik J. S., Szychowski J. A. 1994; Avocado sunblotch disease: A persistent viroid infection in which variants are associated with differential symptoms. Journal of General Virology 75:1543–1549
    [Google Scholar]
  20. Semancik J. S., Weathers L. G. 1972; Exocortis virus: an infectious free-nucleic acid plant virus with unusual properties. Virology 47:456–466
    [Google Scholar]
  21. Semancik J. S., Morris T. J., Weathers L. G. 1975; Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease. Virology 63:160–167
    [Google Scholar]
  22. Semancik J. S., Szychowski J. A., Rakowski A. G., Symons R. H. 1993; Isolates of citrus exocortis viroid recovered by host and tissue selection. Journal of General Virology 74:2427–2436
    [Google Scholar]
  23. Semancik J. S., Szychowski J. A., Rakowski A. G., Symons R. H. 1994; A stable 463 nucleotide variant of citrus exocortis viroid produced by terminal repeats. Journal of General Virology 75:727–732
    [Google Scholar]
  24. Van Dorst H. J. M., Peters D. 1974; Some biological observations on pale fruit, a viroid incited disease of cucumber. Netherlands Journal of Plant Pathology 80:85
    [Google Scholar]
  25. Visvader J. E., Symons R. H. 1983; Comparative sequence and structure of different isolates of citrus exocortis viroid. Virology 130:232–237
    [Google Scholar]
  26. Visvader J. E., Symons R. H. 1985; Eleven new sequence variants of citrus exocortis viroid and the correlation of sequence with pathogenicity. Nucleic Acids Research 13:2907–2920
    [Google Scholar]
  27. Visvader J. E., Symons R. H. 1986; Replication of in vitro constructed viroid mutants: location of the pathogenicity-modulating domain of citrus exocortis viroid. EMBO Journal 5:2051–2055
    [Google Scholar]
  28. Visvader J. E., Gould A. R., Bruening G. E., Symons R. H. 1982; Citrus exocortis viroid: nucleotide sequence and secondary structure of an Australian isolate. FEBS Letters 137:288–292
    [Google Scholar]
  29. Walter B., Thouvenal J. C., Fauquet C. 1980; Les viroses de la tomate en Cote d’Ivoire. Annals de Phytopathologie 12:259
    [Google Scholar]
  30. Weathers L. G., Greer F. C. Jr, Harjungs M. K. 1967; Transmission of exocortis virus of citrus to herbaceous plants. Plant Disease Reporter 51:868–871
    [Google Scholar]
  31. Yang X., Hadidi A., Garnsey S. M. 1992; Enzymatic cDNA amplification of citrus exocortis and cachexia viroids from infected citrus hosts. Phytopathology 82:279–285
    [Google Scholar]
  32. Zuker M. 1989; On finding all suboptimal foldings of an RNA molecule. Science 244:48–52
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-9-2271
Loading
/content/journal/jgv/10.1099/0022-1317-76-9-2271
Loading

Data & Media loading...

Most cited Most Cited RSS feed