1887

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Volume 76, Issue 9

The complete sequence of a cucumber mosaic virus from that is deficient in the replication of satellite RNAs Free

Abstract

A cucumber mosaic virus (CMV-Ix) from is unusual in that it does not support the accumulation of some well-characterized CMV satellite RNAs in plants. CMV-Ix can support a particular satellite RNA variant which causes lethal tomato necrosis when inoculated with other CMV strains but not when inoculated with CMV-Ix. This difference in ability to support accumulation of specific satellite variants is apparent even when their sequences differ by only 10 nucleotides. Electroporation of tomato protoplasts with combinations of CMV-Ix or CMV-1 RNA plus the same satellite variants showed similar differences in accumulation, indicating a defect in satellite RNA replication and not movement or encapsidation. Pseudorecombinant virus infections between CMV-1 and CMV-Ix indicated that the genomic determinants responsible for this phenotype reside on RNA 1 since only combinations with CMV-Ix RNA 1 failed to replicate satellite RNA. The complete genome of CMV-Ix was cloned, sequenced and compared with the genomes of other cucumoviruses. CMV-Ix is most similar in RNA and protein sequence to subgroup 1 CMV-Fny and CMV-Y but slightly less similar than they are to each other. CMV-Ix and all cucumovirus strains sequenced thus far share a domain in the 3′ untranslated portion of their genomic RNAs in which 39 of 40 bases are completely conserved.

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© Journal of General Virology 1995
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1995-09-01
2024-04-20
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The complete sequence of a cucumber mosaic virus from Ixora that is deficient in the replication of satellite RNAs
J. Gen. Virol. 76, 2257 (1995); https://doi.org/10.1099/0022-1317-76-9-2257
/content/journal/jgv/10.1099/0022-1317-76-9-2257
/content/journal/jgv/10.1099/0022-1317-76-9-2257
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