1887

Abstract

A cucumber mosaic virus (CMV-Ix) from is unusual in that it does not support the accumulation of some well-characterized CMV satellite RNAs in plants. CMV-Ix can support a particular satellite RNA variant which causes lethal tomato necrosis when inoculated with other CMV strains but not when inoculated with CMV-Ix. This difference in ability to support accumulation of specific satellite variants is apparent even when their sequences differ by only 10 nucleotides. Electroporation of tomato protoplasts with combinations of CMV-Ix or CMV-1 RNA plus the same satellite variants showed similar differences in accumulation, indicating a defect in satellite RNA replication and not movement or encapsidation. Pseudorecombinant virus infections between CMV-1 and CMV-Ix indicated that the genomic determinants responsible for this phenotype reside on RNA 1 since only combinations with CMV-Ix RNA 1 failed to replicate satellite RNA. The complete genome of CMV-Ix was cloned, sequenced and compared with the genomes of other cucumoviruses. CMV-Ix is most similar in RNA and protein sequence to subgroup 1 CMV-Fny and CMV-Y but slightly less similar than they are to each other. CMV-Ix and all cucumovirus strains sequenced thus far share a domain in the 3′ untranslated portion of their genomic RNAs in which 39 of 40 bases are completely conserved.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-9-2257
1995-09-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/9/JV0760092257.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-9-2257&mimeType=html&fmt=ahah

References

  1. Avila-Rincon M. J., Collmer C. W., Kaper J. M. 1986; In vitro translation of cucumoviral satellites. I. Purification and nucleotide sequence of cucumber mosaic virus-associated RNA 5 from cucumber mosaic virus strain S. Virology 152:446–454
    [Google Scholar]
  2. Bernal J. J., Moriones E., García-Arenal F. 1991; Evolutionary relationships in the cucumoviruses: nucleotide sequence of tomato aspermy virus RNA 1. Journal of General Virology 72:2191–2195
    [Google Scholar]
  3. Boccard F., Baulcombe D. 1993; Mutational analysis of cis-acting sequences and gene function in RNA3 of cucumber mosaic virus. Virology 193:563–578
    [Google Scholar]
  4. Collmer C. W., FIowell S. H. 1992; Role of satellite RNA in the expression of symptoms caused by plant viruses. Annual Review of Phytopathology 30:419–442
    [Google Scholar]
  5. Collmer C. W., Stenzler L., Chen X., Fay N., Hacker D., Howell S. H. 1992; Single amino acid change in the helicase domain of the putative RNA replicase of turnip crinkle virus alters symptom intensification by virulent satellites. Proceedings of the National Academy of Sciences, USA 89:309–313
    [Google Scholar]
  6. Davies C., Symons R. H. 1988; Further implications for the evolutionary relationships between tripartite plant viruses based on cucumber mosaic virus RNA 3. Virology 165:216–224
    [Google Scholar]
  7. Ding S., Anderson B. J., Haase H. R., Symons R. H. 1994; New overlapping gene encoded by the cucumber mosaic virus genome. Virology 198:593–601
    [Google Scholar]
  8. Doolittle S. P. 1916; A new infectious mosaic disease of cucumber. Phytopathology 6:145–147
    [Google Scholar]
  9. Dreher T. W., Bujarski J. J., Hall T. C. 1984; Mutant viral RNAs synthesized in vitro show altered aminoacylation and replicase template activities. Nature 311:171–175
    [Google Scholar]
  10. Habili N., Symons R. H. 1989; Evolutionary relationship between luteoviruses and other RNA plant viruses based on sequence motifs in their putative RNA polymerases and nucleic acid helicases. Nucleic Acids Research 17:9543–9555
    [Google Scholar]
  11. Harrison B. D., Mayo M. A., Baulcombe D. C. 1987; Virus resistance in transgenic plants that express cucumber mosaic virus satellite RNA. Nature 328:799–802
    [Google Scholar]
  12. Hayes R. J., Buck K. W. 1990; Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:363–368
    [Google Scholar]
  13. Hayes R. J., Tousch D., Jacquemond M., Pereira V. C., Buck K. W., Tepfer M. 1992; Complete replication of a satellite RNA in vitro by a purified RNA-dependent RNA polymerase. Journal of General Virology 73:1597–1600
    [Google Scholar]
  14. Higgins D. G., Sharp P. M. 1989; Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS 5:151–153
    [Google Scholar]
  15. Jacquemond M., Amselem J., Tepfer M. 1988; A gene coding for a monomeric form of cucumber mosaic virus satellite RNA confers tolerance to CMV. Molecular Plant-Microbe Interactions 1:311–316
    [Google Scholar]
  16. Joshi R. L., Joshi S., Chapeville F., Haenni A. L. 1983; tRNA-like structures of plant viral RNAs: conformational requirements for adenylation and aminoacylation. EMBO Journal 2:1123–1127
    [Google Scholar]
  17. Kaper J. M., Tousignant M. E. 1977; Cucumber mosaic virus-associated RNA 5. I. Role of host plant and helper strain in determining amount of associated RNA 5 with virions. Virology 80:186–195
    [Google Scholar]
  18. Kaper J. M., Tousignant M. E., Thompson S. M. 1981; Cucumber mosaic virus-associated RNA 5. VIII. Identification and partial characterization of a CARNA 5 incapable of inducing tomato necrosis. Virology 114:526–533
    [Google Scholar]
  19. Kaper J. M., Gallitelli D., Tousignant M. E. 1990a; Identification of a 334-ribonucleotide viral satellite as principal aetiological agent in a tomato necrosis epidemic. Research in Virology 141:81–95
    [Google Scholar]
  20. Kaper J. M., Tousignant M. E., Geletka L. M. 1990b; Cucumber mosaic virus-associated RNA 5. XII. Symptom modulating effect is codetermined by satellite replication support function of helper virus. Research in Virology 141:487–503
    [Google Scholar]
  21. Karasawa A., Nakaho K., Kakutani T., Minobe Y., Ehara Y. 1991; Nucleotide sequence of RNA 3 of peanut stunt cucumovirus. Virology 185:464–467
    [Google Scholar]
  22. Karasawa A., Nakaho K., Kakutani T., Minobe Y., Ehara Y. 1991; Nucleotide sequence analyses of peanut stunt cucumovirus RNAs 1 and 2. Journal of General Virology 73:701–707
    [Google Scholar]
  23. Kataoka J., Masuta C., Takanami Y. 1990a; Complete nucleotide sequence of RNA 1 of cucumber mosaic virus Y strain and evolutionary relationships among genome RNAs of the virus strains. Annals of the Phytopathological Society of Japan 56:501–507
    [Google Scholar]
  24. Kataoka J., Masuta C., Takanami Y. 1990b; Complete nucleotide sequence of RNA 2 of cucumber mosaic virus Y strain. Annals of the Phytopathological Society of Japan 56:495–500
    [Google Scholar]
  25. Lahser F. C., Marsh L. E., Hall T. C. 1993; Contributions of the brome mosaic virus RNA-33′-nontranslated region to replication and translation. Journal of Virology 67:3295–3303
    [Google Scholar]
  26. McGarvey P. B., Kaper J. M., Avila-Rincon M. J., Peña L., Diaz-Ruiz J. R. 1990; Transformed tomato plants express a satellite RNA of cucumber mosaic virus and produce lethal necrosis upon infection with viral RNA. Biochemical and Biophysical Research Communications 170:548–555
    [Google Scholar]
  27. McGarvey P. B., Montasser M. S., Kaper J. M. 1994; Transgenic tomato plants expressing satellite RNA are tolerant to some strains of cucumber mosaic virus. Journal of the American Society for Horticultural Science 119:642–647
    [Google Scholar]
  28. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  29. Mi S., Durbin R. K., Huang H. V., Rice C., Stollar V. 1989; Association of the Sinbis virus RNA methyltransferase activity with nonstructural protein nsPl. Journal of Virology 170:358–391
    [Google Scholar]
  30. Montasser M. S., Tousignant M. E., Kaper J. M. 1991; Satellite-mediated protection of tomato against cucumber mosaic virus. I. Greenhouse experiments and simulated epidemic conditions in the field. Plant Disease 75:86–92
    [Google Scholar]
  31. Moriones E., Fraile A., Garcia-Arenal F. 1991a; Host-associated selection of sequence variants from a satellite RNA of cucumber mosaic virus. Virology 184:465–168
    [Google Scholar]
  32. Moriones E., Roossinck M. J., Garcia-Arenal F. 1991b; Nucleotide sequence of tomato aspermy virus RNA 2. Journal of General Virology 72:779–783
    [Google Scholar]
  33. Nitta N., Masuta C., Kuwata S., Takanami Y. 1988a; Comparative studies on the nucleotide sequence of cucumber mosaic virus RNA 3 between Y strain and Q strain. Annals of the Phytopathological Society of Japan 54:516–522
    [Google Scholar]
  34. Nitta N., Takanami Y., Kuwata S., Kubo S. 1988b; Inoculation with RNAs 1 and 2 of cucumber mosaic virus induces viral RNA replicase activity in tobacco mesophyll protoplasts. Journal of General Virology 69:2695–2700
    [Google Scholar]
  35. O’Reilly D., Thomas C. J. R., Coutts R. H. A. 1991; Tomato aspermy virus has an evolutionary relationship with other tripartite RNA plant viruses. Journal of General Virology 72:1–7
    [Google Scholar]
  36. Owen J., Shintaku M., Aeschleman P., Ben Tahar S., Palukaitis P. 1990; Nucleotide sequence and evolutionary relationships of cucumber mosaic virus (CMV) strains: CMV RNA 3. Journal of General Virology 71:2243–2249
    [Google Scholar]
  37. Palukaitis P., Roossinck M. J., Dietzgen R. G., Francki R. I. B. 1992; Cucumber mosaic virus. Advances in Virus Research 41:281–348
    [Google Scholar]
  38. Piazzolla P., Diaz-Ruiz J. R., Kaper J. M. 1979; Nucleic acid homologies of eighteen cucumber mosaic virus isolates determined by competition hybridization. Journal of General Virology 45:361–369
    [Google Scholar]
  39. Pogue G. P., Hall T. C. 1992; The requirement for a 5′ stem-loop structure in brome mosaic virus replication supports a new model for viral positive-strand RNA initiation. Journal of Virology 66:674–684
    [Google Scholar]
  40. Rezaian M. A., Williams R. H. V., Symons R. H. 1985; Nucleotide sequence of cucumber mosaic virus RNA 1. Presence of a sequence complementary to part of the viral satellite RNA and homologies with other viral RNAs. European Journal of Biochemistry 150:331–339
    [Google Scholar]
  41. Rizzo T. M., Palukaitis P. 1988; Nucleotide sequence and evolutionary relationships of cucumber mosaic virus (CMV) strains: CMV RNA 2. Journal of General Virology 69:1777–1787
    [Google Scholar]
  42. Rizzo T. M., Palukaitis P. 1989; Nucleotide sequence and evolutionary relationships of cucumber mosaic virus (CMV) strains: CMV RNA 1. Journal of General Virology 70:1–11
    [Google Scholar]
  43. Roossinck M. J., Palukaitis P. 1991; Differential replication in zucchini squash of a cucumber mosaic virus satellite RNA maps to RNA 1 of the helper virus. Virology 181:371–373
    [Google Scholar]
  44. Sayama H., Sato T., Kominato M., Natsuaki T., Kaper J. M. 1991; Field testing of a satellite-containing attenuated strain of cucumber mosaic virus for tomato protection in Japan. Phytopathology 83:405–410
    [Google Scholar]
  45. Smith C. R., Tousignant M. E., Kaper J. M. 1992; Replication footprint analysis of cucumber mosaic virus electroporated into tomato protoplasts. Analytical Biochemistry 200:310–314
    [Google Scholar]
  46. Suzuki M., Kuwata S., Kataoka J., Masuta C., Nitta N., Takanami Y. 1991; Functional analysis of deletion mutants of cucumber mosaic virus RNA3 using an in vitro transcription system. Virology 183:106–113
    [Google Scholar]
  47. Tien P., Wu G. 1991; Satellite RNA for the biological control of plant disease. Advances in Virus Research 39:321–339
    [Google Scholar]
  48. Waterworth H. E., Povish W. R. 1975; A virus related to cucumber mosaic virus isolated from imported Ixora plants. Phytopathology 65:728–729
    [Google Scholar]
  49. Wu G., Kaper J. M., Jaspars E. M. J. 1991; Replication of cucumber mosaic virus satellite RNA in vitro by an RNA-dependent RNA polymerase from virus infected tobacco. FEBS Letters 292:213–216
    [Google Scholar]
  50. Wu G., Kaper J. M., Kung S. 1993; Replication of satellite RNA in vitro by homologous and heterologous cucumoviral RNA-dependent RNA polymerases. Biochimie 75:749–755
    [Google Scholar]
  51. Zhao S. Z., Wang X., Wang G. J., Tien P. 1990; Transgenic tomato resistant to cucumber mosaic virus by expressing its monomer and dimer satellite cDNA. Scientia Sinica (B) 7:708–713
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-9-2257
Loading
/content/journal/jgv/10.1099/0022-1317-76-9-2257
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error