@article{mbs:/content/journal/jgv/10.1099/0022-1317-76-9-2235, author = "Bernard, Serge and Laude, Hubert", title = "Site-specific alteration of transmissible gastroenteritis virus spike protein results in markedly reduced pathogenicity", journal= "Journal of General Virology", year = "1995", volume = "76", number = "9", pages = "2235-2241", doi = "https://doi.org/10.1099/0022-1317-76-9-2235", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-76-9-2235", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The pathogenicity of neutralization-resistant mutants of the enteric coronavirus transmissible gastroenteritis virus (TGEV) was examined in the newborn piglet. The parental virus (Purdue-115 strain), as well as several mutants selected using monoclonal antibodies (MAbs) directed to antigenic sites A and B, caused an acute enteritis with 100% mortality. By contrast, most of the site D (MAb 40.1) mutants exhibited a strongly reduced enteropathogenicity, leading to the survival of animals inoculated with up to 1000-fold the 100% lethal dose of parental virus. Such a phenotypical change was correlated with point mutations or a small deletion, all located within the S gene sequence coding for the Pro-145 to Cys-155 segment of the mature polypeptide. These observations suggest that an N-terminal subregion of the S molecule is an essential determinant for pathogenesis in TGEV infection.", }