Complete nucleotide sequence of the herpesvirus simiae glycoprotein G gene and its expression as an immunogenic fusion protein in bacteria Free

Abstract

The nucleotide sequence of a 2384 bp portion within the unique short (U) region of the herpesvirus simiae (simian herpes B virus; SHBV) genome is presented. A partial and a complete open reading frame (ORF) were found within this nucleotide sequence. The partial ORF encodes the C terminus (147 amino acids) of a protein kinase which is highly conserved in the herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) and simian agent 8 (SA8) U regions. The complete ORF is located 3′ to the partial ORF within the 2384 bp sequence and encodes a 593 amino acid glycoprotein which appears to be closely related to the SA8 glycoprotein G (gG), but shares little amino acid similarity with gG of HSV-1 and -2. However, the complete ORF shares certain features conserved among most alphaherpesvirus gGs, notably three highly conserved cysteine residues and an adjacent -glycosylation site. Therefore, it was concluded that this complete ORF encodes the SHBV gG. The 358 amino acid C-terminal portion of SHBV gG was expressed in as a fusion protein and this was detected by immunoblotting with sera from cynomolgus monkeys which were either experimentally or naturally infected with SHBV. The purified fusion protein was inoculated into rabbits to raise an antiserum which recognized a number of apparently SHBV gG-specific protein bands in extracts from SHBV-infected simian cells.

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1995-09-01
2024-03-28
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References

  1. Ashley R. L. 1993; Laboratory techniques in the diagnosis of herpes simplex infection. Genitourinary Medicine 69:174–183
    [Google Scholar]
  2. Balachandran N., Hutt-Fletcher L. M. 1985; Synthesis and processing of glycoprotein gG of herpes simplex virus type 2. Journal of Virology 54:825–832
    [Google Scholar]
  3. Bennett A. M., Harrington L., Kelly D. C. 1992; Nucleotide sequence analysis of genes encoding glycoproteins D and J in simian herpes B virus. Journal of General Virology 73:2963–2967
    [Google Scholar]
  4. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  5. Colle C. F. III, Flowers C. C., O’Callaghan D. J. 1992; Open reading frames encoding protein kinase, homolog of glycoprotein gX of pseudorabies virus, and a novel glycoprotein map within the unique short segment of equine herpesvirus type 1. Virology 188:545–557
    [Google Scholar]
  6. Crabb B. S., Nagesha H. S., Studdert M. J. 1992; Identification of equine herpesvirus 4 glycoprotein G: a type-specific, secreted glycoprotein. Virology 190:143–154
    [Google Scholar]
  7. Cropper L. M., Lees D. N., Patt R., Sharp I. R., Brown D. 1992; Monoclonal antibodies for the identification of herpesvirus simiae (B virus). Archives of Virology 123:267–277
    [Google Scholar]
  8. Dall’Olio F., Malagolini N., Campadelli-Fiume G., Serafini-Cessi F. 1987; Glycosylation pattern of herpes simplex type 2 glycoprotein G from precursor species to the mature form. Archives of Virology 97:237–249
    [Google Scholar]
  9. Eberle R., Black D., Hilliard J. K. 1989; Relatedness of glycoproteins expressed on the surface of simian herpesvirus virions and infected cells to specific HSV glycoproteins. Archives of Virology 109:233–252
    [Google Scholar]
  10. Eberle R., Zhang M., Black D. H. 1993; Gene mapping and sequence analysis of the unique short region of the simian herpesvirus SA8 genome. Archives of Virology 130:391–411
    [Google Scholar]
  11. Fierer J., Bazeley P., Braud A. I. 1973; Herpes B virus encephalomyelitis presenting as ophthalic zoster. Annals of Internal Medicine 79:225–228
    [Google Scholar]
  12. Harrington L., Wall L. V. M., Kelly D. C. 1992; Molecular cloning and physical mapping of the genome of simian herpes B virus and comparison of genome organization with that of herpes simplex virus type 1. Journal of General Virology 73:1217–1226
    [Google Scholar]
  13. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted break-points for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  14. Higgins D. G., Sharp P. M. 1988; CLUSTAL: a package for performing multiple sequence alignments on a microcomputer. Gene 73:237–244
    [Google Scholar]
  15. Hilliard J. K., Black D., Eberle R. 1989; Simian alphaherpes-viruses and their relation to the human herpes simplex viruses. Archives of Virology 109:83–102
    [Google Scholar]
  16. Kongsuwan K., Johnson M. A., Prideaux C. T., Sheppard M. 1993; Identification of an infectious laryngotracheitis virus gene encoding an immunogenic protein with a predicted Mr of 32 kilodaltons. Virus Research 29:125–140
    [Google Scholar]
  17. Lees D. N., Baskerville A., Cropper L. M., Brown D. W. G. 1991; Herpesvirus simiae (B virus) antibody response and virus shedding in experimental primary infection of cynomolgus monkeys. Laboratory Animal Science 41:360–364
    [Google Scholar]
  18. Lipman D. J., Pearson W. R. 1985; Rapid and sensitive protein similarity searches. Science 227:1435–1441
    [Google Scholar]
  19. Ludwig H., Pauli G., Gelderblom H., Darai G., Koch H.-G., Flugel R. M., Norrild B., Daniel M. D. 1983; B virus (Herpesvirus simiae). In The Herpesviruses vol 2 pp 385–428 Edited by Roizman B. New York and London: Plenum Press;
    [Google Scholar]
  20. McGeoch D. J. 1990; Evolutionary relationships of virion glycoprotein genes in the S regions of alphaherpesvirus genomes. Journal of General Virology 71:2361–2367
    [Google Scholar]
  21. McGeoch D. J., Dolan A., Donald S., Rixon F. J. 1985; Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. Journal of Molecular Biology 181:1–13
    [Google Scholar]
  22. McGeoch D. J., Moss H. W. M., McNab D., Frame M. C. 1987; DNA sequence and genetic content of the HindIII l region in the short unique component of the herpes simplex virus type 2 genome: identification of the gene encoding glycoprotein G, and evolutionary comparisons. Journal of General Virology 68:19–38
    [Google Scholar]
  23. Palmer A. E. 1987; B virus, herpesvirus simiae: historical perspective. Journal of Medical Primatology 16:99–130
    [Google Scholar]
  24. Rea T. J., Timmins J. G., Long G. W., Post L. E. 1985; Mapping and sequence of the gene for the pseudorabies virus glycoprotein which accumulates in the medium of infected cells. Journal of Virology 54:21–29
    [Google Scholar]
  25. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Scinicariello F., English W. J., Hilliard J. 1993; Identification by PCR of meningitis caused by herpes B virus. Lancet 341:1660–1661
    [Google Scholar]
  27. Serafini-Cessi F., Malagolini N., Dall’Olio F., Pereira L., Campadelli-Fiume G. 1985; Oligosaccharide chains of herpes simplex virus type 2 glycoprotein gG. 2. Archives of Biochemistry and Biophysics 240:866–876
    [Google Scholar]
  28. Slomka M. J., Brown D. W. G., Clewley J. P., Bennett A. M., Harrington L., Kelly D. C. 1993; Polymerase chain reaction for detection of herpesvirus simiae (B virus) in clinical specimens. Archives of Virology 131:89–99
    [Google Scholar]
  29. Spatz S. J., Rota P. A., Maes R. K. 1994; Identification of the feline herpesvirus type 1 (FHV-1) genes encoding glycoproteins G, D, I and E: expression of FHV-1 glycoprotein D in vaccinia and raccoon poxviruses. Journal of General Virology 75:1235–1244
    [Google Scholar]
  30. Su H. K., Eberle R., Courtney R. J. 1987; Processing of the herpes simplex virus type 2 glycoprotein gG-2 results in secretion of a 34, 000-Mr cleavage product. Journal of Virology 61:1735–1737
    [Google Scholar]
  31. Su H. K., Fetherston J. D., Smith M. E., Courtney R. J. 1993; Orientation of the cleavage site of the herpes simplex virus glycoprotein G-2. Journal of Virology 67:2954–2959
    [Google Scholar]
  32. Telford E. A. R., Watson M. S., McBride K., Davison A. J. 1992; The DNA sequence of equine herpesvirus-1. Virology 189:304–316
    [Google Scholar]
  33. Vizoso A. D. 1975a; Recovery of herpes simiae (B virus) from both primary and latent infections in rhesus monkeys. British Journal of Experimental Pathology 56:485–488
    [Google Scholar]
  34. Vizoso A. D. 1975b; Latency of herpes simiae (B virus) in rabbits. British Journal of Experimental Pathology 56:489–494
    [Google Scholar]
  35. Wall L. V. M., Zwartouw H. T., Kelly D. C. 1989; Discrimination between twenty isolates of herpesvirus simiae (B virus) by restriction enzyme analysis of the viral genome. Virus Research 12:283–296
    [Google Scholar]
  36. Weigler B. J. 1992; Biology of B virus in macaque and human hosts: a review. Clinical Infectious Diseases 14:555–567
    [Google Scholar]
  37. Weldon S. K., Su H. K., Fetherston J. D., Courtney R. J. 1990; In vitro synthesis and processing of herpes simplex virus type 2 gG-2, using cell-free transcription and translation systems. Journal of Virology 64:1357–1359
    [Google Scholar]
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