1887

Abstract

Aujeszky′s disease virus (ADV) is a well known neurotropic virus in pigs. In the present study the mechanism of spread of ADV along the maxillary nerve and the role of the viral envelope glycoproteins gC, gE and gI in this process was examined in pigs. The Ka parental strain of ADV and its gC-, gE- and gI-deleted mutants were inoculated intranasally in pigs, after which virus dissemination in the maxillary nerve and the trigeminal ganglion was monitored at time intervals by means of virus isolation. The parental strain was isolated from both the nasal mucosa and the trigeminal ganglion at 21 h post-inoculation (p.i.), whereas the middle part of the connecting maxillary nerve was positive only after 48 h p.i. It appears, therefore, that ADV travels from the nasal mucosa via the nerve towards the ganglion in a non-infectious form, and then replicates in the neuronal somas, after which infectious virus is transported towards the nasal mucosa. Although all mutants were present at 48 h p.i. in the nasal mucosa and the trigeminal ganglion, the appearance of infectious virus in the maxillary nerve was clearly delayed with the gE and gI mutants. It is suggested that glycoproteins gE and gI are involved in the axonal transport of infectious ADV away from neuronal cell bodies, also called anterograde transport.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-8-2063
1995-08-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/8/JV0760082063.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-8-2063&mimeType=html&fmt=ahah

References

  1. Cook M. L., Stevens J. G. 1973; Pathogenesis of herpetic neuritis and ganglionitis in mice: evidence for intra-axonal transport of infection. Infection and Immunity 7:272–288
    [Google Scholar]
  2. Field H. J., Hill T. J. 1974; The pathogenesis of pseudorabies in mice following peripheral inoculation. Journal of General Virology 23:145–157
    [Google Scholar]
  3. Hill T. J., Field H. J., Roome A. P. C. 1972; Intra-axonal location of herpes simplex virus particles. Journal of General Virology 15:253–255
    [Google Scholar]
  4. Kaplan A. S., Vatter A. E. 1959; A comparison of herpes simplex and pseudorabies viruses. Virology 7:394–A07
    [Google Scholar]
  5. Klein R. J., DeStefano E. 1983; Dissemination of herpes simplex virus in ganglia after footpad inoculation in neurectomized and non-neurectomized mice. Archives of Virology 77:231–238
    [Google Scholar]
  6. Kristensson K., Lycke E., Sjostrand J. 1971; Spread of herpes simplex virus in peripheral nerves. Acta Neuropathologica 17:44–53
    [Google Scholar]
  7. Kritas S. K., Pensaert M. B., Mettenleiter T. C. 1994a; Invasion and spread of single glycoprotein deleted mutants of Aujeszky’s disease virus (ADV) in the trigeminal nervous pathway of pigs after intranasal inoculation. Veterinary Microbiology 40:323–334
    [Google Scholar]
  8. Kritas S. K., Pensaert M. B., Mettenleiter T. C. 1994b; Role of envelope glycoproteins gI, gp63 and gIII in the invasion and spread of Aujeszky’s disease virus in the olfactory nervous pathway of the pig. Journal of General Virology 75:2319–2327
    [Google Scholar]
  9. Lycke E., Kristensson K., Svennerholm B., Vahlne A., Ziegler R. J. 1984; Uptake and transport of herpes simplex virus in neurites of rat dorsal root ganglia cells in culture. Journal of General Virology 65:55–64
    [Google Scholar]
  10. Lycke E., Hamark B., Johansson M., Krotochwil A., Lycke J., Svennerholm B. 1988; Herpes simplex virus infection of the human sensory neuron (an electron microscopy study). Archives of Virology 101:87–104
    [Google Scholar]
  11. McCracken R. M., McFerran J. B., Dow C. 1973; The neural spread of pseudorabies virus in calves. Journal of General Virology 20:17–28
    [Google Scholar]
  12. McFerran J. B., Dow C. 1965; The distribution of the virus of Aujeszky’s disease (pseudorabies virus) in experimentally infected swine. American Journal of Veterinary Research 26:631–635
    [Google Scholar]
  13. Mettenleiter T. C., Schreurs C., Zuckermann F., Ben-Porat T. 1987; Role of pseudorabies virus glycoprotein gI in virus release from infected cells. Journal of Virology 61:2764–2769
    [Google Scholar]
  14. Mettenleiter T. C., Schreurs C., Zuckermann F., Ben-Porat T., Kaplan A. S. 1988; Role of glycoprotein gIII of pseudorabies virus in virulence. Journal of Virology 62:2712–2717
    [Google Scholar]
  15. Nauwynck H. J., Pensaert M. B. 1995; Cell-free and cell-associated viraemia in pigs after oronasal infection with Aujeszky’s disease virus. Veterinary Microbiology 43:307–314
    [Google Scholar]
  16. Sabo A., Rajcani J., Blaskovic D. 1969; Studies on the pathogenesis of Aujeszky’s disease. III. The distribution of the virulent virus in piglets after intranasal infection. Acta Virologica 13:407–414
    [Google Scholar]
  17. Wildy P. 1967; The progression of herpes simplex virus to the central nervous system of the mouse. Journal of Hygiene 65:173–192
    [Google Scholar]
  18. Wittmann G., Jakubik J., Ahl R. 1980; Multiplication and distribution of Aujeszky’s disease (pseudorabies) virus in vaccinated and non-vaccinated pigs after intranasal infection. Archives of Virology 66227–240
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-8-2063
Loading
/content/journal/jgv/10.1099/0022-1317-76-8-2063
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error