1887

Abstract

A virus isolate, designated as 11-Cal, originating from southern France has been identified as an isolate of the mite-transmitted brome streak mosaic rymovirus (BrSMV) by serological and morphological properties. BrSMV is a member of the genus of the family . The complete nucleotide sequence of the RNA genome of BrSMV has been determined. The assembled RNA is 9672 nucleotides in length, excluding a 3′-terminal poly(A)sequence. The RNA contains one open reading frame (ORF) of 9282 nucleotides coding for a polyprotein of 3093 amino acids. A comparison with typical potyviruses showed that BrSMV has a similar genome organization. The predicted cleavage sites of the polyprotein of BrSMV are similar to those of potyviruses. Nevertheless, unusual dipeptides are proposed in two cases. Based on the proposed location of the cleavage sites nine mature proteins are predicted. Specific motifs, described for potyviral polyproteins, are almost all present in the polyprotein of BrSMV, too. However, only an incomplete zinc-finger motif is present in the potential helper component and the motif for aphid transmission in the coat protein is not found. Several alignments of amino acid sequences showed less similarity between BrSMV and potyviruses than between different potyviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-8-2035
1995-08-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/8/JV0760082035.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-8-2035&mimeType=html&fmt=ahah

References

  1. Allison R., Johnston R. E., Dougherty W. G. 1986; The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154:9–20
    [Google Scholar]
  2. Balint R., Plooy I., Steele C. 1990; The nucleotide sequence of zucchini yellow mosaic virus. Abstracts of the VIIIth International Congress of Virology Berlin, p 472
    [Google Scholar]
  3. Berg J. M. 1986; Potential metal-binding domains in nucleic acid binding protein. Science 232:485–487
    [Google Scholar]
  4. Berger P. H., Pirone T. P. 1986; The effect of helper component on the uptake and localization of potyviruses in Myzus persicae . Virology 153:256–261
    [Google Scholar]
  5. Carrington J. C., Dougherty W. C. 1987a; Small nuclear inclusion protein encoded by a plant potyvirus genome is a protease. Journal of Virology 61:2540–2548
    [Google Scholar]
  6. Carrington J. C., Dougherty W. C. 1987b; Processing of the tobacco etch virus 49K protease requires autoproteolysis. Virology 160:355–362
    [Google Scholar]
  7. Carrington J. C., Cary S. M., Parks T. D., Dougherty W. G. 1989; A second proteinase encoded by a plant potyvirus genome. EMBO Journal 8:365–370
    [Google Scholar]
  8. Carrington J. C., Herndon K. L. 1992; Characterization of the potyviral HC-Pro autoproteolytic cleavage site. Virology 187:308–315
    [Google Scholar]
  9. Chang C.-A., Hiebert E., Purcifull D. E. 1988; Analysis of in vitro translation of bean yellow mosaic virus RNA: inhibition of proteolytic processing by an antiserum to the 49K nuclear inclusion protein. Journal of General Virology 69:1117–1122
    [Google Scholar]
  10. Davidson A. D., Prols M., Schell J., Steinbiss H.-H. 1991; The nucleotide sequence of RNA 2 of barley yellow mosaic virus. Journal of General Virology 72:989–993
    [Google Scholar]
  11. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  12. Dinant S., Lot H., Albouy J., Kusiak C., Meyer M., Astier-Manifacier S. 1991; Nucleotide sequence of the 3′-terminal region of lettuce mosaic potyvirus RNA shows a Gln/Val dipeptide at the cleavage site between the polymerase and the coat protein. Archives of Virology 116:235–252
    [Google Scholar]
  13. Domier L. L., Franklin K. M., Shahabuddin M., Hellmann G. M., Overmeyer J. H., Hiremath S. T., Siaw M. F. E., Lomonossoff G. P., Shaw J. G., Rhoads R. E. 1986; The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Research 14:5417–5429
    [Google Scholar]
  14. Dougherty W. G., Carrington J. C. 1988; Expression and function of potyviral gene products. Annual Review of Phytopathology 26:123–143
    [Google Scholar]
  15. Dougherty W. G., Parks T. D. 1991; Post-translational processing of the tobacco etch virus 49-kDa small nuclear inclusion polyprotein: identification of an internal cleavage site and delimination of VPg and proteinase domains. Virology 183:449–456
    [Google Scholar]
  16. Dougherty W. G., Carrington J. C., Cary S. M., Parks T. D. 1988; Biochemical and mutational analysis of a plant virus polyprotein cleavage site. EMBO Journal 7:1281–1287
    [Google Scholar]
  17. Dougherty W. G., Cary S. M., Parks T. D. 1989a; Molecular genetic analysis of a plant virus polyprotein cleavage site: a model. Virology 171:356–364
    [Google Scholar]
  18. Dougherty W. G., Parks T. D., Cary S. M., Bazan J. F., Fletterick R. J. 1989b; Characterization of the catalytic residues of the tobacco etch virus 49-kDa proteinase. Virology 172:302–310
    [Google Scholar]
  19. Gallie D. R., Sleat D. E., Watts J. W., Turner P. C., Wilson T. M. A. 1987; The 5′-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo . Nucleic Acids Research 15:3257–3273
    [Google Scholar]
  20. Garcia J. A., Riechmann J. L., Lain S. 1989; Proteolytic activity of the plum pox potyvirus NIa-like protein in Escherichia coli . Virology 170:362–369
    [Google Scholar]
  21. Ghabrial S. A., Smith H. A., Parks T. D., Dougherty W. G. 1990; Molecular genetic analyses of the soybean mosaic virus NIa proteinase. Journal of General Virology 71:1921–1927
    [Google Scholar]
  22. Gorbalenya A. E., Donchenko A. P., Blinov V. M., Koonin E. V. 1989; Cysteine proteases of positive strand RNA viruses and chymotrypsin-like serine proteases. FEBS Letters 243:103–114
    [Google Scholar]
  23. Gunasinghe U. B., Flasinski S., Nelson R. S., Cassidy B. G. 1994; Nucleotide sequence and genome organization of peanut stripe potyvirus. Journal of General Virology 75:2519–2526
    [Google Scholar]
  24. Hellmann G. M., Shaw J. G., Rhoads R. E. 1988; In vitro analysis of tobacco vein mottling virus NIa cistron: evidence for a virus-encoded protease. Virology 163:554–562
    [Google Scholar]
  25. Jagadish M. N., Ward C. W., Gough K. H., Tulloch P. A., Whittaker L. A., Shukla D. D. 1991; Expression of potyvirus coat protein in Escherichia coli and yeast and its assembly into viruslike particles. Journal of General Virology 72:1543–1550
    [Google Scholar]
  26. Jayaram C., Hill J. H., Miller W. A. 1992; Complete nucleotide sequence of two soybean mosaic virus strains differentiated by response of soybean containing the Rsv resistance gene. Journal of General Virology 73:2067–2077
    [Google Scholar]
  27. Johansen E., Rasmussen O. F., Heide M., Borkhard B. 1991; The complete nucleotide sequence of pea seed-borne mosaic virus RNA. Journal of General Virology 72:2625–2632
    [Google Scholar]
  28. Kamer G., Agros P. 1984; Primary structural comparison of RNA-dependent RNA polymerases from plant, animal and bacterial viruses. Nucleic Acids Research 12:7269–7282
    [Google Scholar]
  29. Kashiwazaki S., Minobe Y., Omura T., Hibino H. 1990; Nucleotide sequence of barley yellow mosaic virus RNA 1: a close evolutionary relationship with potyviruses. Journal of General Virology 71:2781–2790
    [Google Scholar]
  30. Kashiwazaki S., Minobe Y., Hibino H. 1991; Nucleotide sequence of barley yellow mosaic RNA 2. Journal of General Virology 72:995–999
    [Google Scholar]
  31. Lain S., Riechmann J. L., Garcia J. A. 1989; The complete nucleotide sequence of plum pox potyvirus RNA. Virus Research 13:157–172
    [Google Scholar]
  32. Lütcke H. A., Chow K. C., Mickel F. S., Moss K. A., Kern H. F., Scheele G. A. 1987; Selection of AUG initiation codons differs in plants and animals. EMBO Journal 6:43–18
    [Google Scholar]
  33. Maiss E., Breyel E., Brisske A., Casper R. 1988; Molecular cloning of DNA complementary to the RNA-genome of plum pox virus (PPV). Journal of Phytopathology 122:222–231
    [Google Scholar]
  34. Maiss E., Timpe U., Brisske A., Jelkmann W., Casper R., Himmler G., Mattanovich D., Katinger H. W. D. 1989; The complete nucleotide sequence of plum pox virus RNA. Journal of General Virology 70:513–524
    [Google Scholar]
  35. Mavankal G., Rhoads R. E. 1991; In vitro cleavage at or near the N-terminus of the helper component protein in the tobacco vein mottling virus polyprotein. Virology 185:721–731
    [Google Scholar]
  36. Milicic D., Kujundzic M., Wrischer M., Plavsic B. 1980; A potyvirus isolated from Bromus mollis . Acta Botanica Croatica 39:27–32
    [Google Scholar]
  37. Milicic D., Mamula D., Plazibat M. 1982; Some properties of brome streak mosaic virus. Acta Botanica Croatica 41:7–12
    [Google Scholar]
  38. Murphy J. F., Rychlik W., Rhoads R. E., Hunt A. G., Shaw J. G. 1991; A tyrosine residue in the small nuclear inclusion protein of tobacco vein mottling virus links the VPg to the viral RNA. Journal of Virology 65:511–513
    [Google Scholar]
  39. Niblett C. L., Zagula K. R., Calvert L. A., Kendall T. L., Stark D. M., Smith C. E., Beachy R. N., Lommbl S. A. 1991; cDNA cloning and nucleotide sequence of the wheat streak mosaic capsid protein gene. Journal of General Virology 72:499–504
    [Google Scholar]
  40. Nicolas O., Laliberté J. F. 1992; The complete nucleotide sequence of turnip mosaic potyvirus RNA. Journal of General Virology 73:2785–2793
    [Google Scholar]
  41. Oh C. S., Carrington J. C. 1989; Identification of essential residues in potyvirus proteinase HC-pro by site directed mutagenesis. Virology 173:692–699
    [Google Scholar]
  42. Peerenboom E., Pröls M., Schell J., Steinbiss H.-H., Davidson A. D. 1992; The complete nucleotide sequence of RNA 1 of a German isolate of barley yellow mosaic virus and its comparison with a Japanese isolate. Journal of General Virology 73:1303–1308
    [Google Scholar]
  43. Puurand Ü, Mäkinen K., Paulin L., Saarma M. 1994; The nucleotide sequence of potato virus A genomic RNA and its sequence similarities with other potyviruses. Journal of General Virology 75:457–461
    [Google Scholar]
  44. Riechmann J. L., Lain S., Garcia J. A. 1989; The genome-linked protein and 5′ end RNA sequence of plum pox potyvirus. Journal of General Virology 70:2785–2789
    [Google Scholar]
  45. Riechmann J. L., Lain S., Garcia J. A. 1992; Highlights and prospects of potyvirus molecular biology. Journal of Virology 73:1–16
    [Google Scholar]
  46. Robaglia C., Durand-Tardif M., Tronchet M., Boudazin G., Astier-Manifacier S., Casse-Delbart F. 1989; Nucleotide sequence of potato virus Y (N strain) genomic RNA. Journal of General Virology 70:935–947
    [Google Scholar]
  47. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  48. Shukla D. D., Ward C. W. 1988; Amino acid sequence homology of coat proteins as a basis for identification and classification of the potyvirus group. Journal of General Virology 69:2703–2710
    [Google Scholar]
  49. Shukla D. D., Ward C. W. 1989; Identification and classification of potyviruses on the basis of coat protein sequence data and serology. Archives of Virology 106:171–200
    [Google Scholar]
  50. Vance V. B., Moore D., Turpen T. H., Bracker A., Hollowell V. C. 1992; The complete nucleotide sequence of pepper mottle virus genomic RNA: comparison of the encoded polyprotein with those of other sequenced potyviruses. Virology 191:19–30
    [Google Scholar]
  51. Verchot J., Koonin E. V., Carrington J. C. 1991; The 35-kDa protein from the N-terminus of the potyviral polyprotein functions as a third virus-encoded proteinase. Virology 185:527–535
    [Google Scholar]
  52. Yeh S.-D., Jan F.-J., Chiang C.-H., Doong T.-J., Chen M.-C., Chung P.-H., Bau H.-J. 1992; Complete nucleotide sequence and genetic organization of papaya ringspot virus RNA. Journal of General Virology 73:2531–2541
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-76-8-2035
Loading
/content/journal/jgv/10.1099/0022-1317-76-8-2035
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error