1887

Abstract

Complementary DNAs encoding ORFs 2 to 7 of equine arteritis virus (EAV) have been cloned into the expression vector pGEX to produce glutathione--transferase fusion proteins. Recombinant proteins were affinity purified and screened in ELISA with equine sera to identify immunoreactive polypeptides. The large envelope glycoprotein (G) was identified as the most reactive to EAV-positive equine sera and an immuno-dominant epitope was mapped between amino acids 55 and 98 by subcloning and expression. A fusion protein covering this region and a G-specific synthetic peptide (residues 75 through 97) induced EAV-neutralizing antibody in vaccinated horses. The defined antigenic region of G is likely to be exposed on the surface of the native EAV virion and consequently may be useful in the development of diagnostic tests and vaccines.

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1995-08-01
2024-11-06
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References

  1. Balasuriya U. B. R., Rossitto P. V., DeMaula C. D., MacLachlan N. J. 1993; A 29K envelope glycoprotein of equine arteritis virus expresses neutralization determinants recognized by murine monoclonal antibodies. Journal of General Virology 74:2525–2529
    [Google Scholar]
  2. Cavanagh D., Brian D. A., Brinton M., Enjuanes L., Holmes K. V., Horzinek M. C., Lai M. M. C., Laude H., Plagemann P. G. W., Siddell S., Spaan W. J. M., Taguchi F., Talbot P. J. 1994; Revision of the taxonomy of the Coronavirus, Torovirus and Arterivirus genera. Archives of Virology 135:227–237
    [Google Scholar]
  3. Chirnside E. D., Wearing C. M., Binns M. M., Mumford J. A. 1994; Comparison of M and N gene sequences distinguishes variation amongst equine arteritis virus isolates. Journal of General Virology 75:1491–1497
    [Google Scholar]
  4. Chirnside E. D., Francis P. M., de Vries A. A. F., Sinclair R., Mumford J. A. 1995; Development and evaluation of an ELISA using recombinant fusion protein to detect the presence of host antibody to equine arteritis virus (EAV). Journal of Virological Methods (in press)
    [Google Scholar]
  5. Conzelmann K.-K., Visser N., van Woensel P., Thiel H.-J. 1993; Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the arterivirus group. Virology 193:329–339
    [Google Scholar]
  6. Cook R. F., Gann S. J., Mumford J. A. 1989; The effects of vaccination with tissue culture-derived viral vaccines on detection of antibodies to equine arteritis virus by enzyme-linked immunosorbent assay (ELISA). Veterinary Microbiology 20:181–189
    [Google Scholar]
  7. Den Boon J. A., Snijder E. J., Chirnside E. D., de Vries A. A. F., Horzinek M. C., Spaan W. J. M. 1991; Equine arteritis virus is not a togavirus but belongs to the coronavirus-like superfamily. Journal of Virology 65:2910–2920
    [Google Scholar]
  8. Deregt D., de Vries A. A. F., Raamsman M. J. B., Elmgren L. D., Rottier P. J. M. 1994; Monoclonal antibodies to equine arteritis virus proteins identify the GL protein as a target for virus neutralization. Journal of General Virology 75:2439–2444
    [Google Scholar]
  9. De Vries A. A. F., Chirnside E. D., Bredenbeek P. J., Gravestein L. A., Horzinek M. C., Spaan W. J. M. 1990; All subgenomic RNAs of equine arteritis virus contain a common leader sequence. Nucleic Acids Research 18:3241–3247
    [Google Scholar]
  10. De Vries A. A. F., Chirnside E. D., Horzinek M. C., Rottier P. J. M. 1992; The structural proteins of equine arteritis virus. Journal of Virology 66:6294–6303
    [Google Scholar]
  11. Doll E. R., Knappenberger R. E., Bryans J. T. 1957; An outbreak of abortion caused by the equine arteritis virus. Cornell Veterinarian 47:69–75
    [Google Scholar]
  12. Doll E. R., Bryans J. T., Wilson J. C., McCollum W. H. 1968; Immunization against equine viral arteritis using modified live virus propagated in cell cultures of rabbit kidney. Cornell Veterinarian 58:497–524
    [Google Scholar]
  13. Frey M. L., Earnisse K. A., Landgraf J. G., Pearson J. E. 1992; Diagnostic testing for swine infertility and respiratory syndrome virus at the National Veterinary Services Laboratories. In Proceedings of International Symposium on SIRS/PRRS/PEARS Minnesota, 1992, p 25:
    [Google Scholar]
  14. Fukunaga Y., McCollum W. H. 1977; Complement fixation reactions in equine viral arteritis. American Journal of Veterinary Research 38:2043–2046
    [Google Scholar]
  15. Fukunaga Y., Wada R., Matsumura T., Sugiura T., Imagawa H. 1990; Induction of immune response and protection from equine viral arteritis (EVA) by formalin-inactivated virus vaccine for EVA in horses. Journal of Veterinary Medicine 37:135–141
    [Google Scholar]
  16. Fukunaga Y., Wada R., Matsumura T., Anzai T., Imagawa H., Sugiura T., Kumanomido T., Kanemaru T., Kamada M. 1992; An attempt to protect against persistent infection of equine viral arteritis in the reproductive tract of stallions using formalin inactivated-virus vaccine. In Proceedings of the Sixth International Conference on Equine Infectious Diseases, Cambridge 1991 pp 239–244 Edited by Plowright W., Rossdale P. D., Wade J. F. Newmarket: R & W Publication;
    [Google Scholar]
  17. Fukunaga Y., Matsumura T., Sugiura T., Wada R., Imagawa H., Kanemura T., Kamada M. 1994; Use of the serum neutralization test for equine viral arteritis with different virus strains. Veterinary Record 134:574–576
    [Google Scholar]
  18. Gerber H., Steck F., Hofer B., Walther L., Friedli U. 1978; Serological investigations on equine viral arteritis. In Proceedings of the Fourth International Conference on Equine Infectious Diseases, Lyon 1976 pp 461–465 Edited by Bryans J. T., Gerber H. Princeton: Veterinary Publications;
    [Google Scholar]
  19. Gibson T. J. 1984; Studies on the Epstein–Barr virus genome. PhD thesis, University of Cambridge
    [Google Scholar]
  20. Golnik W., Michalska Z., Michalak T. 1981; Natural equine viral arteritis in foals. Schweizer Archiv für Tierheilkunde 123:523–533
    [Google Scholar]
  21. Gullick W. J., Downward J., Waterfield M. D. 1985; Antibodies to the autophosphorylation sites of the epidermal growth factor receptor protein-tyrosine kinase as probes of structure and function. EMBO Journal 4:2869–2877
    [Google Scholar]
  22. Harry T. O., McCollum W. H. 1981; Stability of viability and immunizing potency of lyophilized, modified equine arteritis live-virus vaccine. American Journal of Veterinary Research 42:1501–1505
    [Google Scholar]
  23. Harty J. T., Plagemann G. W. 1988; Formalin inactivation of the lactate dehydrogenase-elevating virus reveals a major neutralizing epitope not recognized during natural infection. Journal of Virology 62:3210–3216
    [Google Scholar]
  24. Horzinek M. C. 1981; Equine arteritis virus. In Non-Arthropod Borne Togaviruses pp 30–31 London: Academic Press;
    [Google Scholar]
  25. Hyllseth B. 1973; Structural proteins of equine arteritis virus. Archiv für die gesamte Virusforschung 40:177–188
    [Google Scholar]
  26. Jameson B. A., Wolf H. 1988; The antigenic index: a novel algorithm for predicting antigenic determinants. Computer Applications in the Biosciences 4:181–186
    [Google Scholar]
  27. Johnson K. S., Harrison G. B. L., Lightowlers M. W., O’Hoy K. L., Cougle W. G., Dempster R. P., Lawrence S. B., Vinton J. G., Heath D. D., Rickard M. D. 1989; Vaccination against ovine cysticercosis using a defined recombinant antigen. Nature 338:585–587
    [Google Scholar]
  28. Karber G. 1931; Beitrag zur kollektiven Behandlung pharma-kologischer. Reihenversuche. Naunyn-Schmiedebergs Archiv für experimented Pathologie und Pharmakologie 162:480–483
    [Google Scholar]
  29. Laemmli U. K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 277:680–685
    [Google Scholar]
  30. Lopez de Turiso J. A., Cortes E., Ranz A., Garcia J., Sanz A., Vela C., Casal J. I. 1991; Fine mapping of canine parvovirus B cell epitopes. Journal of General Virology 72:2445–2456
    [Google Scholar]
  31. McCollum W. H. 1976; Studies of passive immunity in foals to equine viral arteritis. Veterinary Microbiology 1:45–54
    [Google Scholar]
  32. McCollum W. H. 1986; Responses of horses vaccinated with an avirulent modified-live equine arteritis virus propagated in E. derm (NBL-6) cell line to nasal inoculations with virulent virus. American Journal of Veterinary Research 47:1931–1934
    [Google Scholar]
  33. McCollum W. H., Doll E. R., Wilson J. C. 1961; The recovery of virus from horses with experimental cases of equine arteritis using monolayer cell cultures of equine kidney. American Journal of Veterinary Research 23:465–169
    [Google Scholar]
  34. McCollum W. H., Timoney P. J., Roberts A. W., Willard J. E., Carswell G. D. 1988; Responses of vaccinated and non-vaccinated mares to artificial insemination with semen from stallions persistently infected with equine arteritis virus. In Proceedings of the Fifth International Conference on Equine Infectious Diseases, Lexington 1991 pp 13–18 Edited by Powell D. G. Lexington: University Press of Kentucky;
    [Google Scholar]
  35. Murphy F. A. 1980; Togavirus morphology and morphogenesis. In The Togaviruses: Biology, Structure and Replication pp 241–316 Edited by Schlesinger R. W. London: Academic Press;
    [Google Scholar]
  36. Murphy T. W., Timoney P. J., McCollum W. H. 1988; Analysis of genetic variation among strains of equine arteritis virus. In Proceedings of the Fifth International Conference on Equine Infectious Diseases, Lexington 1987 pp 3–12 Edited by Powell D. G. Lexington: University Press of Kentucky;
    [Google Scholar]
  37. Murphy T. W., McCollum W. H., Timoney P. J., Klingeborn B. W., Hyllseth B., Golnik W., Erasmus B. 1992; Genomic variability among globally distributed isolates of equine arteritis virus. Veterinary Microbiology 32:101–115
    [Google Scholar]
  38. Neu S. M., Timoney P. J., McCollum W. H. 1988; Persistent infection of the reproductive tract of stallions persistently infected with equine arteritis virus. In Proceedings of the Fifth International Conference on Equine Infectious Diseases, Lexington 1987 pp 149–154 Edited by Powell D. G. Lexington: University Press of Kentucky;
    [Google Scholar]
  39. Paweska J. T., Barnard B. J. H. 1993; Serological evidence of equine arteritis virus in donkeys in South Africa. Onderstepoort Journal of Veterinary Research 60:155–158
    [Google Scholar]
  40. Plagemann P. G. W., Moennig V. 1992; Lactate dehydrogenase-elevating virus, equine arteritis virus and simian hemorrhagic fever virus: a new group of positive-strand RNA viruses. Advances in Virus Research 41:99–192
    [Google Scholar]
  41. Pognonec P., Kato H., Sumimoto H., Kretzschmar M., Roeder R. G. 1991; A quick procedure for the purification of functional recombinant proteins over-expressed in E. coli . Nucleic Acids Research 19:6650
    [Google Scholar]
  42. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbour Laboratory;
    [Google Scholar]
  43. Senne D. A., Pearson J. E., Carbrey E. A. 1985; Equine viral arteritis: a standard procedure for the virus neutralization test and comparison of results of a proficiency test performed at five laboratories. In Proceedings of the 89th Annual Meeting of the United States Animal Health Association, Milwaukee, Wisconsin, 1985 pp 29–34
    [Google Scholar]
  44. Smith D. B., Johnson K. S. 1988; Single-step purification of polypeptides expressed in Escherichia coli as fusion proteins with glutathione-S-transferase. Gene 67:31–40
    [Google Scholar]
  45. Snijder E. J., Horzinek M. C., Spaan W. J. M. 1994; The coronaviruslike superfamily. In Coronaviruses: Molecular Biology and Virus-Host Interactions pp 235–244 Edited by Laude H., Vautherot J.-F. New York: Plenum Press;
    [Google Scholar]
  46. Stewart J. M., Young J. D. 1984 Solid Phase Peptide Synthesis 2nd edn Rockford: Pierce Chemical;
    [Google Scholar]
  47. Thomas L. M., Huntington P. J., Mead L. J., Wingate D. L., Rogerson B. A., Lew A. M. 1992; A soluble recombinant fusion protein of the transmembrane envelope protein of equine infectious anemia virus for ELISA. Veterinary Microbiology 31:127–137
    [Google Scholar]
  48. Timoney P. J., McCollum W. H. 1991; Equine viral arteritis: current clinical and economical significance. Proceedings of the American Association of Equine Practitioners 36:403–409
    [Google Scholar]
  49. Timoney P. J., McCollum W. H., Roberts A. W., Murphy T. W. 1986; Demonstration of the carrier state in naturally acquired equine arteritis virus infection in the stallion. Research in Veterinary Science 41:279–280
    [Google Scholar]
  50. Timoney P. J., Umphenour N. W., McCollum W. H. 1988; Safety evaluation of a commercial modified live equine arteritis virus vaccine for use in stallions. In Proceedings of the Fifth International Conference on Equine Infectious Diseases, Lexington, 1987 pp 19–27 Edited by Powell D. G. Lexington: University Press of Kentucky;
    [Google Scholar]
  51. Vaala W. E., Hamir A. N., Dubovi E. J., Timoney P. J., Ruiz B. 1992; Fatal, congenitally acquired infection with equine arteritis virus in a neonatal thoroughbred. Equine Veterinary Journal 24:155–158
    [Google Scholar]
  52. Van Berlo M. F., Zeegers J. J. W., Horzinek M. C., van der Zeijst B. A. M. 1983; Antigenic comparison of equine arteritis virus (EAV) and lactic dehydrogenase virus (LDV); binding of staphylococcal protein A to the nucleocapsid protein of EAV. Zentralblatt für Veterinaermedizin Beiheft 30:297–304
    [Google Scholar]
  53. Van Berlo M. F., Rottier P. J. M., Spaan W. J. M., Horzinek M. C. 1986; Equine arteritis virus-induced polypeptide synthesis. Journal of General Virology 67:1543–1549
    [Google Scholar]
  54. Wensvoort G., Kluyver E. P., Pol J. M. A., Wagenaar F., Moorman R. J. M., Hulst M. M., Bloemraad R., den Besten A., Zetstra T., Terpstra C. 1992; Lelystad virus, the cause of porcine epidemic abortion and respiratory syndrome: a review of mystery swine disease research at Lelystad. Veterinary Microbiology 33:185–193
    [Google Scholar]
  55. Zeegers J. J. W., Van Der Zeijst B. A. M., Horzinek M. C. 1976; The structural proteins of equine arteritis virus. Virology 73:200–205
    [Google Scholar]
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