1887

Abstract

A variety of recombinant proteins derived from protein pVI of human adenovirus type 2 (Ad2) were analysed for their ability to bind Ad2 hexon . As pVI is also required for activation of the adenovirus-coded protease, the same pVI derivatives were assessed for their ability to activate recombinant adenovirus-coded 23K protease. Two regions, between amino acid residues 48–74 and 233–239 of pVI, were required for the interaction with hexon. These regions are highly conserved amongst mastadenovirus pVI proteins. Both these regions are capable on their own of binding hexon weakly but must be provided in for strong hexon binding. In addition, we found evidence to indicate that conformation as well as sequence was important for good hexon binding in our assays. Authentic processing of the appropriate recombinant pVI derivatives, by the recombinant protease, was obtained without the addition of other cofactors. These findings are discussed in relation to the role of pVI in triggering the adenovirus maturation pathway.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-8-1959
1995-08-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/8/JV0760081959.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-8-1959&mimeType=html&fmt=ahah

References

  1. Akusjarvi G., Persson H. 1981; Gene and mRNA for precursor polypeptide VI from adenovirus type 2. Journal of Virology 38:469–182
    [Google Scholar]
  2. Akusjarvi G., Alestrom P., Pettersson M., Lager M., Jornvall H., Pettersson U. 1984; The gene for the adenovirus 2 hexon polypeptide. Journal of Biological Chemistry 259:13976–13979
    [Google Scholar]
  3. Anderson C. W., Baum P. R., Gesteland P. F. 1973; Processing of adenovirus 2 induced proteins. Journal of Virology 12:241–252
    [Google Scholar]
  4. Greber U. F., Willetts M., Webster P., Helenius A. 1993; Stepwise dismantling of adenovirus 2 during entry into cells. Cell 75:477–186
    [Google Scholar]
  5. Greirson A. W., Nicholson R., Talbot P., Webster A., Kemp G. 1994; The protease of adenovirus serotype 2 requires cysteine residues for both activation and catalysis. Journal of General Virology 75:2761–2764
    [Google Scholar]
  6. Ishibashi M., Maizel J. V. Jr 1974; The polypeptides of adenovirus. V. Young virions, structural intermediates between top components and aged virions. Virology 57:409–124
    [Google Scholar]
  7. Liddington R. C., Yan Y., Moulai J., Sahli R., Benjamin T. L., FIarrison S. C. 1991; Structure of simian virus 40 at 3.8 Å resolution. Nature 354:278–284
    [Google Scholar]
  8. Mangel W. F., McGrath W. J., Toledo D., Anderson C. W. 1993; Viral DNA and a viral peptide are cofactors of adenovirus virion proteinase activity. Nature 361:274–275
    [Google Scholar]
  9. Matthews D. A., Russell W. C. 1994; Adenovirus protein- protein interactions: hexon and protein VI. Journal of General Virology 75:3365–3374
    [Google Scholar]
  10. Mizra M. A., Weber J. 1982; Structure of adenovirus chromatin. Biochimica et Biophysica Acta 696:76–86
    [Google Scholar]
  11. Russell W. C., Kemp G. D. 1995; The role of adenovirus structural proteins in the regulation of adenovirus infection. In The Molecular Repertoire of Adenoviruses Edited by Doerfler W., Bohm P. Heidelberg: Springer-Verlag;
    [Google Scholar]
  12. Stewart P. L., Fuller S. D., Burnett R. M. 1993; Difference imaging of adenovirus: bridging the resolution gap between X-ray crystallography and electron microscopy. EMBO Journal 12:2589–2599
    [Google Scholar]
  13. Weber J. 1976; Genetic analysis of adenovirus type 2. III. Temperature sensitivity of processing of viral proteins. Journal of Virology 17:462–471
    [Google Scholar]
  14. Webster A., Russell S., Talbot P., Russell W. C., Kemp G. D. 1989; Characterization of the adenovirus proteinase: substrate specificity. Journal of General Virology 70:3225–3234
    [Google Scholar]
  15. Webster A., Hay R. T., Kemp G. D. 1993; The adenovirus protease is activated by a virus-coded disulphide-linked peptide. Cell 72:97–104
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-8-1959
Loading
/content/journal/jgv/10.1099/0022-1317-76-8-1959
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error