Role of GTPase activity of murine Mx1 protein in nuclear localization and anti-influenza virus activity Free

Abstract

Murine Mx1 protein is an interferon-inducible GTPase which localizes in nuclei and inhibits influenza virus infection. Wild-type Mx1 and two mutant Mx1 proteins, each carrying a single mutation either in the GTP-binding motif (S50I) or in the self-assembly motif (C71S), were expressed in MDCK cells. Wild-type Mx1 localized in nuclei, forming small granules with minute dots, and inhibited influenza virus growth. Mutant S50I, which had no GTP-binding or GTPase activities, formed linear structures in nuclei and lacked anti-viral activity, while C71S appeared diffuse in nuclei as minute dots without granules, but retained the inhibitory activity against influenza virus growth. A correlation existed between GTPase activity, intranuclear distribution and antiviral activity. We concluded that GTPase activity is essential for expression of the biological activity of Mx1 protein.

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1995-07-01
2024-03-28
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References

  1. Garber E. A., Hreniuk D. Y., Scheidel L. M., Van Der Ploeg L. H. T. 1993; Mutation in murine Mx1: effects on localization and antiviral activity. Virology 194:715–723
    [Google Scholar]
  2. Kolb E., Laine E., Strehler D., Staeheli P. 1992; Resistance to influenza virus infection of Mx transgenic mice expressing Mx protein under the control of two constitutive promoters. Journal of Virology 66:1709–1716
    [Google Scholar]
  3. Melèn K., Ronni T., Krug R. M., von Bonsdorff C.-H., Julkunen I. 1992; Interferon-induced Mx proteins form oligomers and contain a putative leucine zipper. Journal of Biological Chemistry 267:25898–25907
    [Google Scholar]
  4. Melèn K., Ronni T., Lotta T., Julkunen I. 1994; Enzymatic characterization of interferon-induced antiviral GTPases. Journal of Biological Chemistry 269:1–7
    [Google Scholar]
  5. Nakayama M., Nagata K., Kato A., Ishihama A. 1991; Interferon-inducible mouse Mx1 protein that confers resistance to influenza virus is GTPase. Journal of Biological Chemistry 266:21404–21408
    [Google Scholar]
  6. Nakayama M., Nagata K., Ishihama A. 1992; Enzymatic properties of the mouse Mx1 protein-associated GTPase. Virus Research 22:227–234
    [Google Scholar]
  7. Nakayama M., Yazaki K., Kusano A., Nagata K., Hanai N., Ishihama A. 1993; Structure of mouse Mx1 protein. Journal of Biological Chemistry 268:15033–15038
    [Google Scholar]
  8. Noteborn M., Arnheiter H., Richter-Mann L., Browning H., Weissmann C. 1987; Transport of the murine Mx protein into the nucleus is dependent on a basic carboxy-terminal sequence. Journal of Interferon Research 7:657–669
    [Google Scholar]
  9. Pitossi F., Blank A., Schroder A., Schwarz A., Hussi P., Schwemmle M., Pavlovic J., Staeheli P. 1993; A functional GTP-binding motif is necessary for antiviral activity of Mx1 proteins. Journal of Virology 67:6726–6732
    [Google Scholar]
  10. Staeheli P., Haller O., Boll W., Lindenmann J., Weissmann C. 1986; Mx protein: constitutive expression in 3T3 cells transfected with cloned Mx cDNA confers selective resistance to influenza virus. Cell 44:147–157
    [Google Scholar]
  11. Zürcher T., Pavlovic J., Staeheli P. 1992; Nuclear localization of mouse Mx1 protein is necessary for inhibition of influenza virus. Journal of Virology 66:5059–5066
    [Google Scholar]
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