1887

Abstract

This study was undertaken to determine the prevalence of core-gene-defective hepatitis B virus (HBV) in patients with chronic HBV infection, and the nature and significance of the deletions. PCR was performed on sera from 263 patients with chronic HBV infection. Seventeen (6.5%) patients had smaller band(s) in addition to a band of the expected size. Additional bands were also detected in the follow-up samples from 80% and 3% patients who had respectively, multiple and single bands initially. Six patients were further studied by direct sequencing. Four patients had in-frame deletions leading to loss of codons 79–122 of the core gene. Two patients had identical frameshift deletions of nucleotides 2204–2333 resulting in the loss of the first nine codons of the overlapping P gene. Follow-up samples from three of four patients studied showed deletions identical to those in the initial samples. The persistence of these deletions suggests that they were stable and that they may contribute to the chronicity of infection.

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/content/journal/jgv/10.1099/0022-1317-76-7-1821
1995-07-01
2021-10-25
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References

  1. Birnbaum F., Nassal M. 1990; Flepatitis B virus nucleocapsid assembly: primary structure requirements in the core protein. Journal of Virology 64:3319–3330
    [Google Scholar]
  2. Blum H. E., Galun E., Liang T. J., von Weizsacker F., Wands J. R. 1991; Naturally occurring missense mutation in the polymerase gene terminating hepatitis B virus replication. Journal of Virology 65:1836–1842
    [Google Scholar]
  3. Gerken G., Kremsdorf D., Capel F., Petit M. A., Dauguet C., Manns M. P., Zum Buschenfelde K. H. M., Brechot C. 1991; Hepatitis B defective virus with rearrangements in the preS gene during chronic HBV infection. Virology 183:555–565
    [Google Scholar]
  4. Holland J. J. 1990; Defective viral genomes. In Virology 2nd edn, pp 151–165 Edited by Knipe D. M. New York: Raven Press;
    [Google Scholar]
  5. Horwich A. L., Furtak K., Pugh J., Summers J. 1990; Synthesis of hepadnavirus particles that contain replication-defective duck hepatitis B virus genomes in cultured HuH7 cells. Journal of Virology 64:642–650
    [Google Scholar]
  6. Huang A. S., Baltimore D. 1970; Defective viral particles and viral disease processes. Nature 226:325–327
    [Google Scholar]
  7. Kwok S., Higuchi R. 1989; Avoiding false positives with PCR. Nature 339:237–238 (erratum, Nature 339, 490)
    [Google Scholar]
  8. Lok A. S. F., Akarca U., Greene S. 1994; Mutations in the precore region of hepatitis B virus serve to enhance the stability of the secondary structure of the pre-genome encapsidation signal. Proceedings of the National Academy of Sciences, USA 91:4077–4081
    [Google Scholar]
  9. Melegari M., Bruno S., Wands J. R. 1994; Properties of hepatitis B virus pre-Sl deletion mutants. Virology 199:292–300
    [Google Scholar]
  10. Minami M., Okanoue T., Nakajima E., Yasui K., Kagawa K., Kashima K. 1993; Significance of pre-S region-defective hepatitis B virus that emerged during exacerbation of chronic type B hepatitis. Hepatology 17:558–563
    [Google Scholar]
  11. Nakajima E., Minami M., Ochiya T., Kagawa K., Okanoue T. 1994; PreSl deleted variants of hepatitis B virus in patients with chronic hepatitis. Journal of Hepatology 20:329–335
    [Google Scholar]
  12. Nassal M. 1992; The arginine-rich domain of the hepatitis B virus core protein is required for pregenome encapsidation and productive viral positive-strand DNA synthesis but not for virus assembly. Journal of Virology 66:4107–4116
    [Google Scholar]
  13. Okamoto H., Tsuda F., Mayumi M. 1987; Defective mutants of hepatitis B virus in the circulation of symptom-free carriers. Japanese Journal of Experimental Medicine 57:217–221
    [Google Scholar]
  14. Okamoto H., Wang Y., Tanaka T., Machida A., Miyakawa Y., Mayumi M. 1993; Trans-complementation among naturally occurring deletion mutants of hepatitis B virus and integrated viral DNA for the production of viral particles with mutant genomes in hepatoma cell lines. Journal of General Virology 74:407–414
    [Google Scholar]
  15. Santantonio T., Jung M. C., Schneider R., Fernholz D., Milella M., Monno L., Pastore G., Pape G. R., Will H. 1992; Hepatitis B virus genomes that cannot synthesize pre-S2 proteins occur frequently and as dominant virus populations in chronic carriers in Italy. Virology 188:948–952
    [Google Scholar]
  16. Wakita T., Kakumu S., Shibata M., Yoshioka K., Ito Y., Shinagawa T., Ishikawa T., Takayanagi M., Morishima T. 1991; Detection of pre-C and core region mutants of hepatitis B virus in chronic hepatitis B virus carriers. Journal of Clinical Investigation 88:1793–1801
    [Google Scholar]
  17. Yu M., Summers J. 1991; A domain of the hepadnavirus capsid protein is specifically required for DNA maturation and virus assembly. Journal of Virology 652511–2517
    [Google Scholar]
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