@article{mbs:/content/journal/jgv/10.1099/0022-1317-76-6-1533, author = "Leroux, Caroline and Vuillermoz, Sylvie and Mornex, Jean-François and Greenland, Timothy", title = "Genomic heterogeneity in the pol region of ovine lentiviruses obtained from bronchoalveolar cells of infected sheep from France", journal= "Journal of General Virology", year = "1995", volume = "76", number = "6", pages = "1533-1537", doi = "https://doi.org/10.1099/0022-1317-76-6-1533", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-76-6-1533", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "In order to determine the genomic heterogeneity of ovine lentiviruses, we analysed eight isolates from naturally infected sheep from one geographical region of France. A 475 nt fragment in the region of the pol gene coding for reverse transcriptase was amplified by RT-PCR from RNA directly extracted from uncultured bronchoalveolar lavage cells. The resulting PCR fragments were analysed by restriction enzyme digestion, cloned in a TA vector and sequenced. Restriction enzyme analysis showed distinct patterns from the eight isolates, and sequencing showed them to be closely related in both nucleotide (2.3–8.1% variation) and deduced amino acid (0–6.2% variation) sequences. Their amino acid sequences differed from that of visna-maedi virus complete viral genome sequence K1514 by 12.5–15.3%, but from that of caprine arthritis encephalitis virus (CAEV) viral genome sequence Co by only 4.2–6.9%. Phylogenetic analysis showed that the French isolates form a group related to CAEV Co and distant from previously reported ovine lentivirus sequences from different origins.", }